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Showing 151 - 200 of 1321
Showing 151 - 200 of 1321
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210001 | 1/6 | Homo sapiens | MKN45 |
(d)(U)C Total Exosome Isolation UF |
Li, Bowen | 2021 | 67% | |
Study summaryFull title
All authors
Bowen Li, Yiwen Xia, Jialun Lv, Weizhi Wang, Zhe Xuan, Cen Chen, Tianlu Jiang, Lang Fang, Linjun Wang, Zheng Li, Zhongyuan He, Qingya Li, Li Xie, Shengkui Qiu, Lu Zhang, Diancai Zhang, Hao Xu, Zekuan Xu
Journal
Oncogene
Abstract
Liver metastasis (LM) severely affects gastric cancer (GC) patients' prognosis. Small extracellular (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected with miR-151a-3p reconstitution lentivirus
Focus vesicles
Other / small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Protein markers
EV: Calnexin/ TSG101/ TSPAN8/ CD63
non-EV: Albumin Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MKN45
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell viability (%)
98
Cell count
2 500 000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 100 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
8
Wash: time (min)
70
Wash: Rotor Type
Type 100 Ti
Wash: speed (g)
110000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ TSPAN8/ TSG101
Not detected EV-associated proteins
Calnexin
Not detected contaminants
Albumin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNA sequencing
Database
No
Proteinase treatment
Yes
Moment of Proteinase treatment
Before
Proteinase type
Proteinase K
Proteinase concentration
20
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
124,3
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 1,60E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210001 | 2/6 | Homo sapiens | MKN45 |
(d)(U)C Total Exosome Isolation UF |
Li, Bowen | 2021 | 67% | |
Study summaryFull title
All authors
Bowen Li, Yiwen Xia, Jialun Lv, Weizhi Wang, Zhe Xuan, Cen Chen, Tianlu Jiang, Lang Fang, Linjun Wang, Zheng Li, Zhongyuan He, Qingya Li, Li Xie, Shengkui Qiu, Lu Zhang, Diancai Zhang, Hao Xu, Zekuan Xu
Journal
Oncogene
Abstract
Liver metastasis (LM) severely affects gastric cancer (GC) patients' prognosis. Small extracellular (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
transfected with miR-151a-3p reconstitution lentivirus
Focus vesicles
Other / small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Protein markers
EV: Calnexin/ TSG101/ TSPAN8/ CD63
non-EV: Albumin Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MKN45
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell viability (%)
98
Cell count
2 500 000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 100 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
8
Wash: time (min)
70
Wash: Rotor Type
Type 100 Ti
Wash: speed (g)
110000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Total Exosome Isolation
EV-subtype
Distinction between multiple subtypes
Size
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ TSPAN8/ TSG101
Not detected EV-associated proteins
Calnexin
Not detected contaminants
Albumin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNA sequencing
Database
No
Proteinase treatment
Yes
Moment of Proteinase treatment
Before
Proteinase type
Proteinase K
Proteinase concentration
20
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
124,3
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 1,60E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210001 | 5/6 | Homo sapiens | Blood plasma |
(d)(U)C Total Exosome Isolation UF |
Li, Bowen | 2021 | 67% | |
Study summaryFull title
All authors
Bowen Li, Yiwen Xia, Jialun Lv, Weizhi Wang, Zhe Xuan, Cen Chen, Tianlu Jiang, Lang Fang, Linjun Wang, Zheng Li, Zhongyuan He, Qingya Li, Li Xie, Shengkui Qiu, Lu Zhang, Diancai Zhang, Hao Xu, Zekuan Xu
Journal
Oncogene
Abstract
Liver metastasis (LM) severely affects gastric cancer (GC) patients' prognosis. Small extracellular (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
gastric cancer patients with liver metastasis
Focus vesicles
Other / small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Protein markers
EV: Calnexin/ TSG101/ TSPAN8/ CD63
non-EV: Albumin Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 100 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
8
Wash: time (min)
70
Wash: Rotor Type
Type 100 Ti
Wash: speed (g)
110000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSPAN8/ CD63/ TSG101
Not detected EV-associated proteins
Calnexin
Not detected contaminants
Albumin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
No
Proteinase treatment
Yes
Moment of Proteinase treatment
Before
Proteinase type
Proteinase K
Proteinase concentration
20
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
125,5
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2,00E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210001 | 6/6 | Homo sapiens | Blood plasma |
(d)(U)C Total Exosome Isolation UF |
Li, Bowen | 2021 | 67% | |
Study summaryFull title
All authors
Bowen Li, Yiwen Xia, Jialun Lv, Weizhi Wang, Zhe Xuan, Cen Chen, Tianlu Jiang, Lang Fang, Linjun Wang, Zheng Li, Zhongyuan He, Qingya Li, Li Xie, Shengkui Qiu, Lu Zhang, Diancai Zhang, Hao Xu, Zekuan Xu
Journal
Oncogene
Abstract
Liver metastasis (LM) severely affects gastric cancer (GC) patients' prognosis. Small extracellular (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
gastric cancer patients with liver metastasis
Focus vesicles
Other / small extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Protein markers
EV: Calnexin/ TSG101/ TSPAN8/ CD63
non-EV: Albumin Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 100 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
8
Wash: time (min)
70
Wash: Rotor Type
Type 100 Ti
Wash: speed (g)
110000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSPAN8/ CD63/ TSG101
Not detected EV-associated proteins
Calnexin
Not detected contaminants
Albumin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
No
Proteinase treatment
Yes
Moment of Proteinase treatment
Before
Proteinase type
Proteinase K
Proteinase concentration
20
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
125,5
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2,00E+10
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV200186 | 1/1 | Homo sapiens | primary amnion epithelial cells |
(d)(U)C Filtration UF |
Radnaa, Enkhtuya | 2021 | 67% | |
Study summaryFull title
All authors
Enkhtuya Radnaa, Lauren S Richardson, Samantha Sheller-Miller, Tuvshintugs Baljinnyam, Mariana de Castro Silva, Ananth Kumar Kammala, Rheanna Urrabaz-Garza, Talar Kechichian, Sungjin Kim, Arum Han, Ramkumar Menon
Journal
Lab Chip
Abstract
Preterm birth (PTB; <37 weeks of gestation) impacts ∼11% of all pregnancies and contributes to 1 m (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Protein markers
EV: CD63/ CD81/ HMGB1/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary amnion epithelial cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
960
Pelleting: rotor type
Type 70.1Ti
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
5
Wash: time (min)
60
Wash: Rotor Type
Type 70.1Ti
Wash: speed (g)
100,000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100,000
Membrane type
Polypropylene;Other
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HMGB1
Detected EV-associated proteins
CD9/ CD63/ HMGB1/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
mode;Other
Reported size (nm)
127
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size
70
EV-concentration
No
|
||||||||
EV200181 | 3/3 | Homo sapiens | Bronchoalveolar lavage fluid | (d)(U)C | Dlugolecka, Magdalena | 2021 | 67% | |
Study summaryFull title
All authors
Magdalena Dlugolecka, Jacek Szymanski, Lukasz Zareba, Zuzanna Homoncik, Joanna Domagala-Kulawik, Malgorzata Polubiec-Kownacka, Malgorzata Czystowska-Kuzmicz
Journal
Cells
Abstract
The current lack of reliable methods for quantifying extracellular vesicles (EVs) isolated from comp (show more...)
EV-METRIC
67% (78th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bronchoalveolar lavage fluid
Sample origin
lung cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD81/ PD-L1/ Syntenin/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Bronchoalveolar lavage fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
2h
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
110000
Wash: volume per pellet (ml)
8
Wash: time (min)
60
Wash: Rotor Type
Type 70.1Ti
Wash: speed (g)
110000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ TSG101/ Syntenin/ PD-L1/ CD81
Not detected contaminants
Calnexin
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Fluorescent NTA
Relevant measurements variables specified?
NA
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9/ CD63
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
172
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 8.85E+08
Particle analysis: flow cytometry
Flow cytometer type
BD FACSVerse 8 color Flow Cytometer (BD)
Hardware adjustment
Calibration bead size
4.5
Report type
Not Reported
EM
EM-type
Cryo-EM
Image type
Close-up
|
||||||||
EV200159 | 1/4 | Homo sapiens | Expi293F |
DG (d)(U)C |
Lázaro-Ibáñez, Elisa | 2021 | 67% | |
Study summaryFull title
All authors
Elisa Lázaro-Ibáñez, Farid N Faruqu, Amer F Saleh, Andreia M Silva, Julie Tzu-Wen Wang, Janusz Rak, Khuloud T Al-Jamal, Niek Dekker
Journal
ACS Nano
Abstract
The ability to track extracellular vesicles (EVs) in vivo without influencing their biodistribution (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Density gradient
(Differential) (ultra)centrifugation Protein markers
EV: Alix/ CD63/ Flotillin1/ CD9/ CD81
non-EV: Lamin B1 Proteomics
no
EV density (g/ml)
1.10 - 1.13
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Expi293F
EV-harvesting Medium
Serum free medium
Cell viability (%)
85
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
9
Lowest density fraction
10%
Highest density fraction
50%
Total gradient volume, incl. sample (mL)
17
Sample volume (mL)
1
Orientation
Bottom-up
Rotor type
SW 32.1 Ti
Speed (g)
120000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
94
Pelleting: duration (min)
180
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
120000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ Alix/ CD81
Not detected contaminants
Lamin B1
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
126-154
EV concentration
Yes
Particle yield
No NA
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
56
|
||||||||
EV200157 | 7/10 | Homo sapiens | MDA-MB-468 |
(d)(U)C SEC (non-commercial) Polymer-based precipitation |
Martínez-Greene, Juan A | 2021 | 67% | |
Study summaryFull title
All authors
Juan A Martínez-Greene, Karina Hernández-Ortega, Ricardo Quiroz-Baez, Osbaldo Resendis-Antonio, Israel Pichardo-Casas, David A Sinclair, Bogdan Budnik, Alfredo Hidalgo-Miranda, Eileen Uribe-Querol, María Del Pilar Ramos-Godínez, Eduardo Martínez-Martínez
Journal
J Extracell Vesicles
Abstract
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Polymer-based precipitation Protein markers
EV: CD9/ CD63/ CD81/ Alix/ TSG101/ ANXA2/ ANXA5
non-EV: Albumin Proteomics
no
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MDA-MB-468
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
1.50E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
39
Pelleting: rotor type
TLA-100.3
Pelleting: speed (g)
118000
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
Polymer-based precipitation
EV-subtype
Distinction between multiple subtypes
SEC fraction
Used subtypes
F5-10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81/ Alix/ TSG101/ ANXA2/ ANXA5
Detected contaminants
Albumin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
138
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 4.91E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200157 | 8/10 | Homo sapiens | MDA-MB-468 |
(d)(U)C SEC (non-commercial) Polymer-based precipitation DG |
Martínez-Greene, Juan A | 2021 | 67% | |
Study summaryFull title
All authors
Juan A Martínez-Greene, Karina Hernández-Ortega, Ricardo Quiroz-Baez, Osbaldo Resendis-Antonio, Israel Pichardo-Casas, David A Sinclair, Bogdan Budnik, Alfredo Hidalgo-Miranda, Eileen Uribe-Querol, María Del Pilar Ramos-Godínez, Eduardo Martínez-Martínez
Journal
J Extracell Vesicles
Abstract
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Polymer-based precipitation Density gradient Protein markers
EV: CD9/ CD63/ CD81/ Alix/ TSG101/ ANXA2/ ANXA5
non-EV: Albumin Proteomics
no
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MDA-MB-468
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
1.50E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
39
Pelleting: rotor type
TLA-100.3
Pelleting: speed (g)
118000
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
Polymer-based precipitation
EV-subtype
Distinction between multiple subtypes
SEC fraction
Used subtypes
F11-16
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected EV-associated proteins
Alix/ TSG101/ ANXA2/ ANXA5
Detected contaminants
Albumin
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
129
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 5.19E+10
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200157 | 9/10 | Homo sapiens | gingival primary fibroblasts |
(d)(U)C SEC (non-commercial) Polymer-based precipitation |
Martínez-Greene, Juan A | 2021 | 67% | |
Study summaryFull title
All authors
Juan A Martínez-Greene, Karina Hernández-Ortega, Ricardo Quiroz-Baez, Osbaldo Resendis-Antonio, Israel Pichardo-Casas, David A Sinclair, Bogdan Budnik, Alfredo Hidalgo-Miranda, Eileen Uribe-Querol, María Del Pilar Ramos-Godínez, Eduardo Martínez-Martínez
Journal
J Extracell Vesicles
Abstract
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Polymer-based precipitation Protein markers
EV: CD9/ CD63/ CD81/ Alix/ TSG101/ ANXA2/ ANXA5
non-EV: Albumin Proteomics
no
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
gingival primary fibroblasts
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
1.50E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
39
Pelleting: rotor type
TLA-100.3
Pelleting: speed (g)
118000
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
Polymer-based precipitation
EV-subtype
Distinction between multiple subtypes
SEC fraction
Used subtypes
F5-10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81/ ANXA2/ ANXA5
Not detected EV-associated proteins
Alix/ TSG101
Detected contaminants
Albumin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
149
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.89E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200157 | 10/10 | Homo sapiens | gingival primary fibroblasts |
(d)(U)C SEC (non-commercial) Polymer-based precipitation DG |
Martínez-Greene, Juan A | 2021 | 67% | |
Study summaryFull title
All authors
Juan A Martínez-Greene, Karina Hernández-Ortega, Ricardo Quiroz-Baez, Osbaldo Resendis-Antonio, Israel Pichardo-Casas, David A Sinclair, Bogdan Budnik, Alfredo Hidalgo-Miranda, Eileen Uribe-Querol, María Del Pilar Ramos-Godínez, Eduardo Martínez-Martínez
Journal
J Extracell Vesicles
Abstract
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Polymer-based precipitation Density gradient Protein markers
EV: CD9/ CD63/ CD81/ Alix/ TSG101/ ANXA2/ ANXA5
non-EV: Albumin Proteomics
no
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
gingival primary fibroblasts
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
1.50E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
39
Pelleting: rotor type
TLA-100.3
Pelleting: speed (g)
118000
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
Polymer-based precipitation
EV-subtype
Distinction between multiple subtypes
SEC fraction
Used subtypes
F11-16
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected EV-associated proteins
Alix/ TSG101/ ANXA2/ ANXA5
Detected contaminants
Albumin
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
158.5
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 4.68E+10
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200121 | 1/6 | Homo sapiens | HT29 colon carcinoma |
(d)(U)C DG |
Keulers, Tom | 2021 | 67% | |
Study summaryFull title
All authors
Tom G Keulers, Sten F Libregts, Joel E J Beaumont, Kim G Savelkouls, Johan Bussink, Hans Duimel, Ludwig Dubois, Marijke I Zonneveld, Carmen López-Iglesias, Karel Bezstarosti, Jeroen A Demmers, Marc Vooijs, Marca Wauben, Kasper M A Rouschop
Journal
J Extracell Vesicles
Abstract
Tumour hypoxia is a hallmark of solid tumours and contributes to tumour progression, metastasis deve (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT29 colon carcinoma
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell count
1.00E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.0M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.15
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
12.4
Pelleting: duration (min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected EV-associated proteins
CD81/ CD63/ CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
100
Particle analysis: flow cytometry
Flow cytometer type
influx
Hardware adjustment
customized influx
Calibration bead size
100
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200121 | 2/6 | Homo sapiens | HT29 colon carcinoma |
(d)(U)C DG |
Keulers, Tom | 2021 | 67% | |
Study summaryFull title
All authors
Tom G Keulers, Sten F Libregts, Joel E J Beaumont, Kim G Savelkouls, Johan Bussink, Hans Duimel, Ludwig Dubois, Marijke I Zonneveld, Carmen López-Iglesias, Karel Bezstarosti, Jeroen A Demmers, Marc Vooijs, Marca Wauben, Kasper M A Rouschop
Journal
J Extracell Vesicles
Abstract
Tumour hypoxia is a hallmark of solid tumours and contributes to tumour progression, metastasis deve (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
gabarapl1 KD hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT29 colon carcinoma
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell count
1.00E+06
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.0M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.15
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
12.4
Pelleting: duration (min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected EV-associated proteins
CD81/ CD63/ CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
200
Particle analysis: flow cytometry
Flow cytometer type
influx
Hardware adjustment
customized influx
Calibration bead size
100
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200121 | 4/6 | Homo sapiens | U87 glioblastoma |
(d)(U)C DG |
Keulers, Tom | 2021 | 67% | |
Study summaryFull title
All authors
Tom G Keulers, Sten F Libregts, Joel E J Beaumont, Kim G Savelkouls, Johan Bussink, Hans Duimel, Ludwig Dubois, Marijke I Zonneveld, Carmen López-Iglesias, Karel Bezstarosti, Jeroen A Demmers, Marc Vooijs, Marca Wauben, Kasper M A Rouschop
Journal
J Extracell Vesicles
Abstract
Tumour hypoxia is a hallmark of solid tumours and contributes to tumour progression, metastasis deve (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
gabarapl1 KD hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
U87 glioblastoma
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell count
1.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.4M
Highest density fraction
2.0M
Total gradient volume, incl. sample (mL)
12.4
Sample volume (mL)
0.15
Orientation
Bottom-up
Rotor type
SW 41 Ti
Speed (g)
200000
Duration (min)
960
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
12.4
Pelleting: duration (min)
60
Pelleting: rotor type
SW 41 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected EV-associated proteins
CD81/ CD63/ CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
influx
Hardware adjustment
customized influx
Calibration bead size
100
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200103 | 1/2 | Sus scrofa | seminal plasma |
(d)(U)C Filtration |
Ding, Yaqun | 2021 | 67% | |
Study summaryFull title
All authors
Yaqun Ding, Ning Ding, Yu Zhang, Shenmin Xie, Mengna Huang, Xiangdong Ding, Wuzi Dong, Qin Zhang, Li Jiang
Journal
Front Cell Dev Biol
Abstract
Seminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these (show more...)
EV-METRIC
67% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
seminal plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ Alix/ CD63/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Biomarker/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Sus scrofa
Sample Type
seminal plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
270
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
35
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
108.7
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 660000000000
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200103 | 2/2 | Sus scrofa | seminal plasma |
(d)(U)C Filtration |
Ding, Yaqun | 2021 | 67% | |
Study summaryFull title
All authors
Yaqun Ding, Ning Ding, Yu Zhang, Shenmin Xie, Mengna Huang, Xiangdong Ding, Wuzi Dong, Qin Zhang, Li Jiang
Journal
Front Cell Dev Biol
Abstract
Seminal plasma contains a large number of extracellular vesicles (EVs). However, the roles of these (show more...)
EV-METRIC
67% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
seminal plasma
Sample origin
low sperm motility
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: TSG101/ Alix/ CD63/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Biomarker/Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Sus scrofa
Sample Type
seminal plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
270
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
35
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
120000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
108.7
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 660000000000
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200076 | 1/5 | microalgae | Dinoflagellate | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Dinoflagellate
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-actin/ enolase/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
92
NTA
Report type
Size range/distribution
Reported size (nm)
125
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 6.00E+09
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 2/5 | microalgae | Diatom | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Diatom
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
enolase/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
135
NTA
Report type
Size range/distribution
Reported size (nm)
90
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.40E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 3/5 | microalgae | Glaucophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Glaucophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ HSP70/ beta-actin/ enolase
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
125
NTA
Report type
Size range/distribution
Reported size (nm)
122
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.00E+09
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 4/5 | microalgae | Haptophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ HSP70/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Haptophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ enolase
Not detected EV-associated proteins
HSP70
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
87
NTA
Report type
Size range/distribution
Reported size (nm)
165
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 1.30E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200076 | 5/5 | microalgae | Chlorophyte | (d)(U)C | Picciotto, Sabrina | 2021 | 67% | |
Study summaryFull title
All authors
Sabrina Picciotto, Maria E. Barone, David Fierli, Anita Aranyos, Giorgia Adamo, Darja Božič, Daniele P. Romancino, Christopher Stanly, Rachel Parkes, Svenja Morsbach, Samuele Raccosta, Carolina Paganini, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Umberto Capasso Palmiero, Pamela Santonicola, Ales Iglič, Meiyu Gai, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Katharina Landfester, Giovanna L. Liguori, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Mauro Manno, Nicolas Touzet, Antonella Bongiovanni
Journal
Biomaterials science
Abstract
Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeuti (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ beta-actin/ enolase
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
microalgae
Sample Type
Cell culture supernatant
EV-producing cells
Chlorophyte
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1 mg dry weight biomass/ml
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
beta-actin/ enolase/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
75
NTA
Report type
Size range/distribution
Reported size (nm)
137
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.60E+08
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
||||||||
EV200075 | 1/2 | Tetraselmis chuii | Tetraselmis chuii | (d)(U)C | Adamo, Giorgia | 2021 | 67% | |
Study summaryFull title
All authors
Giorgia Adamo, David Fierli, Daniele P Romancino, Sabrina Picciotto, Maria E Barone, Anita Aranyos, Darja Božič, Svenja Morsbach, Samuele Raccosta, Christopher Stanly, Carolina Paganini, Meiyu Gai, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Loredana Randazzo, Rachel Parkes, Umberto Capasso Palmiero, Estella Rao, Angela Paterna, Pamela Santonicola, Ales Iglič, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Giovanna L Liguori 10 , Katharina Landfester, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni
Journal
J Extracell Vesicles
Abstract
Cellular, inter-organismal and cross kingdom communication via extracellular vesicles (EVs) is inten (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small Extracellular Vesicles - Algosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ H/ ATPase/ enolase/ beta-actin/ HSP70
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
Tetraselmis chuii
Sample Type
Cell culture supernatant
EV-producing cells
Tetraselmis chuii
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
mg dry weight microalgal biomass
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 28
Pelleting: speed (g)
118000
Wash: volume per pellet (ml)
32
Wash: time (min)
70
Wash: Rotor Type
SW 28
Wash: speed (g)
118000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
H/ ATPase/ enolase/ beta-actin/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
90
NTA
Report type
Size range/distribution
Reported size (nm)
130+/-20
EV concentration
Yes
Particle yield
number of particles per mg dry weight microalgal mass 2.00E+09
EM
EM-type
Atomic force-EM/ Transmission-EM/ Scanning-EM/ Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV200039 | 1/4 | Homo sapiens | primary B cells |
(d)(U)C Filtration DG |
Albanese, Manuel | 2021 | 67% | |
Study summaryFull title
MicroRNAs are minor constituents of extracellular vesicles that are rarely delivered to target cells
All authors
Manuel Albanese, Yen-Fu Adam Chen, Corinna Hu, Kathrin Gärtner, Takanobu Tagawa, Ernesto Mejias-Perez, Oliver T. Keppler, Christine Göbel, Reinhard Zeidler, Mikhail Shein, Anne K. Schütz, Wolfgang Hammerschmidt
Journal
PLoS Genetics
Abstract
Mammalian cells release different types of vesicles, collectively termed extracellular vesicles (EVs (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
EBV-infected LCLs
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration DG Protein markers
EV: TSG101/ LMP1
non-EV: Calnexin Proteomics
no
EV density (g/ml)
1.05
Show all info
Study aim
Identification of content (omics approaches)/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary B cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
0%
Highest density fraction
42%
Total gradient volume, incl. sample (mL)
4
Sample volume (mL)
0.38
Orientation
Bottom-up
Rotor type
SW 60 Ti
Speed (g)
160000
Duration (min)
1080
Fraction volume (mL)
0.4
Fraction processing
None
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
LMP1/ TSG101
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV200047 | 2/2 | Homo sapiens | MCF7 |
UF DG |
Yildizhan, Yagmur | 2021 | 67% | |
Study summaryFull title
All authors
Yagmur Yildizhan, Venkata Suresh Vajrala, Edward Geeurickx, Charles Declerck, Nevena Duskunovic, Delphine De Sutter, Sam Noppen, Filip Delport, Dominique Schols, Johannes V. Swinnen, Sven Eyckerman, An Hendrix, Jeroen Lammertyn, Dragana Spasic
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) have drawn huge attention for diagnosing myriad of diseases, including (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Rab27B-GFP
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
Density gradient Protein markers
EV: None
non-EV: None Proteomics
no
EV density (g/ml)
1.086-1.119
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF7
EV-harvesting Medium
Serum free medium
Separation Method
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
11
Lowest density fraction
6
Highest density fraction
18
Total gradient volume, incl. sample (mL)
15
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 32.1 Ti
Speed (g)
186700
Duration (min)
116
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
16
Pelleting: duration (min)
180
Pelleting: rotor type
SW 32.1 Ti
Pelleting: speed (g)
100000
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Characterization: Protein analysis
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV200004 | 2/10 | Homo sapiens | MEC 1 |
DC (d)(U)C |
Elgamal, Sara | 2021 | 67% | |
Study summaryFull title
All authors
Sara Elgamal, Emanuele Cocucci, Ellen J Sass, Xiaokui M Mo, Angela R Blissett, Edward P Calomeni, Kerry A Rogers, Jennifer A Woyach, Seema A Bhat, Natarajan Muthusamy, Amy J Johnson, Karilyn T Larkin, John C Byrd
Journal
JCI insight
Abstract
In chronic lymphocytic leukemia (CLL) and very likely all cancer types, extracellular vesicles (EVs) (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
RPMI
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV: TSG101/ CD81/ CD63/ GAPDH
non-EV: Calnexin/ Albumin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MEC 1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
88
Cell count
2.60E+08 +/- 7.59E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
22
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Density cushion
Density medium
Iodixanol
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ GAPDH/ TSG101/ CD81
Not detected contaminants
Calnexin/ Albumin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
Particles/ug;Yes, other: 1.11E+09 +/- 6.52E+08
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200004 | 3/10 | Homo sapiens | MEC 1 | (d)(U)C | Elgamal, Sara | 2021 | 67% | |
Study summaryFull title
All authors
Sara Elgamal, Emanuele Cocucci, Ellen J Sass, Xiaokui M Mo, Angela R Blissett, Edward P Calomeni, Kerry A Rogers, Jennifer A Woyach, Seema A Bhat, Natarajan Muthusamy, Amy J Johnson, Karilyn T Larkin, John C Byrd
Journal
JCI insight
Abstract
In chronic lymphocytic leukemia (CLL) and very likely all cancer types, extracellular vesicles (EVs) (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
AIMV
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD81/ CD63/ GAPDH
non-EV: Calnexin/ Albumin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MEC 1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
88
Cell count
2.60E+08 +/- 7.59E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
22
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ GAPDH/ CD81
Not detected EV-associated proteins
TSG101
Detected contaminants
Albumin
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
Particles/ug;Yes, other: 5.97E+08 +/- 4.36E+08
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200004 | 4/10 | Homo sapiens | MEC 1 | (d)(U)C | Elgamal, Sara | 2021 | 67% | |
Study summaryFull title
All authors
Sara Elgamal, Emanuele Cocucci, Ellen J Sass, Xiaokui M Mo, Angela R Blissett, Edward P Calomeni, Kerry A Rogers, Jennifer A Woyach, Seema A Bhat, Natarajan Muthusamy, Amy J Johnson, Karilyn T Larkin, John C Byrd
Journal
JCI insight
Abstract
In chronic lymphocytic leukemia (CLL) and very likely all cancer types, extracellular vesicles (EVs) (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Bioreactor
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD52/ TSG101/ CD63/ CD45/ GAPDH/ CD81/ MHC2/ CD19
non-EV: Calnexin/ Albumin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MEC 1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
88
Cell count
2.60E+08 +/- 7.59E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
22
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
GAPDH/ CD63/ TSG101/ CD81
Not detected contaminants
Albumin/ Calnexin
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ CD19/ CD45/ CD52/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
Particles/ug;Yes, other: 2.4E+09 +/- 3.64E+08
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200004 | 9/10 | Homo sapiens | Blood plasma | (d)(U)C | Elgamal, Sara | 2021 | 67% | |
Study summaryFull title
All authors
Sara Elgamal, Emanuele Cocucci, Ellen J Sass, Xiaokui M Mo, Angela R Blissett, Edward P Calomeni, Kerry A Rogers, Jennifer A Woyach, Seema A Bhat, Natarajan Muthusamy, Amy J Johnson, Karilyn T Larkin, John C Byrd
Journal
JCI insight
Abstract
In chronic lymphocytic leukemia (CLL) and very likely all cancer types, extracellular vesicles (EVs) (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
DUC
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD45/ GAPDH/ Alix/ CD81/ CD235A/ HSP70/ MHC2/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
22
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ GAPDH/ TSG101/ CD9/ CD63/ HSP70
Not detected contaminants
Calnexin
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD235A/ CD45/ MHC2/ CD9/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
Particles/ug;Yes, other: 3.40E+08 +/- 2.85E+08
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200004 | 10/10 | Homo sapiens | Blood plasma | (d)(U)C | Elgamal, Sara | 2021 | 67% | |
Study summaryFull title
All authors
Sara Elgamal, Emanuele Cocucci, Ellen J Sass, Xiaokui M Mo, Angela R Blissett, Edward P Calomeni, Kerry A Rogers, Jennifer A Woyach, Seema A Bhat, Natarajan Muthusamy, Amy J Johnson, Karilyn T Larkin, John C Byrd
Journal
JCI insight
Abstract
In chronic lymphocytic leukemia (CLL) and very likely all cancer types, extracellular vesicles (EVs) (show more...)
EV-METRIC
67% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Opti
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ CD63/ CD45/ GAPDH/ Alix/ CD81/ CD235A/ HSP70/ MHC2/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
22
Wash: time (min)
70
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ GAPDH/ HSP70/ TSG101/ CD63/ CD9
Not detected contaminants
Calnexin
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
MHC2/ CD235A/ CD45/ CD81/ CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
EV concentration
Yes
Particle yield
Particles/ug;Yes, other: 2.46E+08 +/- 7.57E+07
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV190107 | 2/3 | Homo sapiens | Jurkat |
DG (d)(U)C |
Martin-Jaular, Lorena | 2021 | 67% | |
Study summaryFull title
All authors
Lorena Martin-Jaular, Nathalie Nevo, Julia P Schessner, Mercedes Tkach, Mabel Jouve, Florent Dingli, Damarys Loew, Kenneth W Witwer, Matias Ostrowski, Georg H H Borner, Clotilde Théry
Journal
EMBO J
Abstract
Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surro (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Protein markers
EV: "cd45/ AChE/ CD63/ CD9/ syntenin-1"
non-EV: Proteomics
no
EV density (g/ml)
1.001-1.097
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Jurkat
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
87
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
106750
Wash: volume per pellet (ml)
37
Wash: time (min)
90
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
106750
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
6%
Highest density fraction
18%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 41 Ti
Speed (g)
200000
Duration (min)
60
Fraction volume (mL)
1 or 2
Fraction processing
Centrifugation
Pelleting: volume per fraction
37
Pelleting: duration (min)
90
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
106750
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
"Other;Gel stain free assay"
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
"CD9/ CD63/ syntenin-1/ cd45"
Not detected EV-associated proteins
AChE
Characterization: Lipid analysis
No
|
||||||||
EV190048 | 1/1 | Homo sapiens | NA |
DC (d)(U)C Filtration |
Shaihov-Teper, Olga | 2021 | 67% | |
Study summaryFull title
All authors
Olga Shaihov-Teper, Eilon Ram, Nimer Ballan, Rafael Y Brzezinski, Nili Naftali-Shani, Rula Masoud, Tamar Ziv, Nir Lewis, Yeshai Schary, La-Paz Levin-Kotler, David Volvovitch, Elchanan M Zuroff, Sergei Amunts, Neta Regev-Rudzki, Leonid Sternik, Ehud Raanani, Lior Gepstein, Jonathan Leor
Journal
Circulation
Abstract
Background: The role of epicardial fat (eFat)-derived extracellular vesicles (EVs) in the pathogenes (show more...)
EV-METRIC
67% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Epicardial fat tissue culture supernatant
Sample origin
cardiac disease
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Filtration Protein markers
EV: TSG101/ CD63/ CD81/ IL-10/ Alix/ TNF-A/ IL1A/ CD9
non-EV: Calreticulin Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Epicardial fat tissue culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
960
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
39
Wash: time (min)
70
Wash: Rotor Type
Type 50
Wash: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ TSG101/ Alix/ CD81
Detected contaminants
Calreticulin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TNF-A/ IL-10/ IL1A
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
1 tablet per 10 ml extraction solution
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-170
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
30-170
|
||||||||
EV220194 | 1/6 | Mus musculus | Liver tissue |
(d)(U)C Filtration ExoQuick |
Matejovič A | 2021 | 63% | |
Study summaryFull title
All authors
Matejovič A, Wakao S, Kitada M, Kushida Y, Dezawa M
Journal
FEBS Open Bio
Abstract
Extracellular vesicles (EVs), which are nanosized vesicles released by cells as intracellular messen (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Liver tissue
Sample origin
Intact liver - CCL4-induced hepatotoxicity
Focus vesicles
Extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration ExoQuick Protein markers
EV: Alix/ CD63/ HSP70/ TSG101
non-EV: Calnexin/ RPL5 Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Liver tissue
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
0.2 or 0.22 µm
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD63/ HSP70/ TSG101
Detected contaminants
Calnexin
Not detected contaminants
Calnexin/ RPL5
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
346 ± 152.9 nm
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
Extra information
Tissue processing: ex vivo culture of intact tissue (48 h)
|
||||||||
EV220162 | 1/1 | Homo sapiens | Serum |
Hydrophobic interaction chromatography UF |
Ji X | 2021 | 63% | |
Study summaryFull title
All authors
Ji X, Huang S, Zhang J, Bruce TF, Tan Z, Wang D, Zhu J, Marcus RK, Lubman DM
Journal
Electrophoresis
Abstract
We have developed a rapid, low-cost, and simple separation strategy to separate extracellular vesicl (show more...)
EV-METRIC
63% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Hydrophobic interaction chromatography
Ultrafiltration Protein markers
EV: CD9/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Ultra filtration
Cut-off size (kDa)
10/ 100
Membrane type
Regenerated cellulose
Other
Name other separation method
Hydrophobic interaction chromatography
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9/ CD63
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mode
Reported size (nm)
112.3
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: 1.00e+11
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
30-150
|
||||||||
EV210462 | 1/3 | Homo sapiens | PLX stromal cells |
(d)(U)C Tangential flow filtration |
Wolf, Martin | 2021 | 63% | |
Study summaryFull title
All authors
Martin Wolf, Rodolphe W Poupardin, Patricia Ebner-Peking, André Cronemberger Andrade, Constantin Blöchl, Astrid Obermayer, Fausto Gueths Gomes, Balazs Vari, Essi Eminger, Heide-Marie Binder, Anna M Raninger, Sarah Hochmann, Gabriele Brachtl, Andreas Spittler, Thomas Heuser, Racheli Ofir, Christian G Huber, Zami Aberman, Katharina Schallmoser, Hans-Dieter Volk, Dirk Strunk
Journal
bioRxiv
Abstract
Nanoparticles can acquire a protein corona defining their biological identity. Corona functions were (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Tangential flow filtration Protein markers
EV: CD9/ CD63/ CD81/ Flotillin-1/ CD49e/ CD44/ CD29/ uPA/ DPP IV/ IGBP-3/ Serpin E1/ PF4/ TSP-1/ Serpin F1/ TF/ PTX3/ CD105
non-EV: GRP94/ Calnexin/ Albumin/ ApoA1 Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-?related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
PLX stromal cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
tangential flow filtration
Cell count
60000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Filtration steps
Between 800 g and 10,000 g
Other
Name other separation method
Tangential flow filtration
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
1000-38000
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81/ Flotillin-1
Detected contaminants
Albumin/ ApoA1
Not detected contaminants
GRP94/ Calnexin
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81/ CD49e/ CD44/ CD29
Proteomics database
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected EV-associated proteins
uPA/ DPP IV/ IGBP-3/ Serpin E1/ PF4/ TSP-1/ Serpin F1/ TF/ PTX3/ CD105
Characterization: RNA analysis
RNA analysis
Type
Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Modus
Reported size (nm)
141
EV concentration
Yes
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV210292 | 1/1 | Homo sapiens | Urine |
Filtration qEV |
Sedej, Ivana | 2021 | 63% | |
Study summaryFull title
All authors
Ivana Sedej, Magda Tušek Žnidarič, Vita Dolžan, Metka Lenassi, Miha Arnol
Journal
Clinical Nephrology
Abstract
Aims: Long-term kidney allograft survival requires a personalized approach to allograft injury recog (show more...)
EV-METRIC
63% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Kidney transplantation
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
qEV Protein markers
EV: CD63/ Flotillin-1/ Tubulin/ HSC70/ GAPDH/ CD9/ CD81
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
Filtration steps
0.2 or 0.22 µm
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
7.5µg (from 60 mL urine)
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ Flotillin-1/ Tubulin/ HSC70/ GAPDH
Not detected EV-associated proteins
CD9/ CD81
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
171 nm
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 8.64e+8
EM
Image type
Close-up, Wide-field
|
||||||||
EV210162 | 1/2 | Homo sapiens | HCT116 |
Total Exosome Isolation UF Filtration |
Clerici, Stefano | 2021 | 63% | |
Study summaryFull title
All authors
Stefano Piatto Clerici 1 , Maikel Peppelenbosch 2 , Gwenny Fuhler 2 , Sílvio Roberto Consonni 1 , Carmen Veríssima Ferreira-Halder 1
Journal
Front Cell Dev Biol
Abstract
Colorectal cancer (CRC) is in the top 10 cancers most prevalent worldwide, affecting equally men and (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
Commercial method
UF Filtration Protein markers
EV: Alix/ TSG101/ B-actin/ CD63/ CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HCT116
EV-harvesting Medium
Serum free medium
Cell viability (%)
80
Cell count
1.50E+10
Separation Method
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
B-actin/ TSG101/ CD81
Not detected EV-associated proteins
CD63/ Alix
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
135
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 81
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
135
|
||||||||
EV210162 | 2/2 | Homo sapiens | HT29 |
Total Exosome Isolation UF Filtration |
Clerici, Stefano | 2021 | 63% | |
Study summaryFull title
All authors
Stefano Piatto Clerici 1 , Maikel Peppelenbosch 2 , Gwenny Fuhler 2 , Sílvio Roberto Consonni 1 , Carmen Veríssima Ferreira-Halder 1
Journal
Front Cell Dev Biol
Abstract
Colorectal cancer (CRC) is in the top 10 cancers most prevalent worldwide, affecting equally men and (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
Commercial method
UF Filtration Protein markers
EV: TSG101/ CD63/ CD81/ Lmwptp/ Alix/ B-actin
non-EV: GM130 Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT29
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
1.30E+10
Separation Method
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Regenerated cellulose
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Lmwptp/ B-actin/ TSG101/ CD81
Not detected EV-associated proteins
CD63/ Alix
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
140
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 214
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
140
|
||||||||
EV210144 | 1/9 | Homo sapiens | Blood plasma |
(d)(U)C DC |
Kumar, Awanit | 2021 | 63% | |
Study summaryFull title
All authors
Awanit Kumar, Surendar Reddy Dhadi, Ngoc‐Nu Mai, Catherine Taylor, Jeremy W. Roy, David A. Barnett, Stephen M. Lewis, Anirban Ghosh, and Rodney J. Ouellette
Journal
J Extracell Vesicles
Abstract
Several studies have demonstrated the potential uses of extracellular vesicles (EVs) for liquid biop (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
DC Protein markers
EV: CD63/ Flotillin1/ CD9/ HSC70
non-EV: FCN3/ SAA/ APOB/ APOA1/ TETN Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density cushion
Density medium
Sucrose
Sample volume
10
Cushion volume
0.7
Density of the cushion
30%
Centrifugation time
120
Centrifugation speed
138,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ HSC70
Detected contaminants
APOB/ FCN3
Not detected contaminants
APOA1/ TETN/ SAA
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
222.1 +/- 6.1
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.18E+08
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210144 | 2/9 | Homo sapiens | Blood plasma |
(d)(U)C Chitosan-based |
Kumar, Awanit | 2021 | 63% | |
Study summaryFull title
All authors
Awanit Kumar, Surendar Reddy Dhadi, Ngoc‐Nu Mai, Catherine Taylor, Jeremy W. Roy, David A. Barnett, Stephen M. Lewis, Anirban Ghosh, and Rodney J. Ouellette
Journal
J Extracell Vesicles
Abstract
Several studies have demonstrated the potential uses of extracellular vesicles (EVs) for liquid biop (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Chitosan-based Protein markers
EV: CD63/ Flotillin1/ CD9/ HSC70
non-EV: FCN3/ APOA1/ TETN Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Other
Name other separation method
Chitosan-based
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ HSC70
Not detected contaminants
APOA1/ FCN3/ TETN
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
89.8 +/- 2.5
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.94E+08
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV210144 | 8/9 | Homo sapiens | HEK293 |
(d)(U)C DG |
Kumar, Awanit | 2021 | 63% | |
Study summaryFull title
All authors
Awanit Kumar, Surendar Reddy Dhadi, Ngoc‐Nu Mai, Catherine Taylor, Jeremy W. Roy, David A. Barnett, Stephen M. Lewis, Anirban Ghosh, and Rodney J. Ouellette
Journal
J Extracell Vesicles
Abstract
Several studies have demonstrated the potential uses of extracellular vesicles (EVs) for liquid biop (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD63/ CD9
non-EV: None Proteomics
yes
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell count
7.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
12
Lowest density fraction
10%
Highest density fraction
90%
Total gradient volume, incl. sample (mL)
12.1
Sample volume (mL)
0.1
Orientation
Bottom-up
Rotor type
SW 40 Ti
Speed (g)
200,000
Duration (min)
960
Fraction volume (mL)
2
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: duration (min)
90
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
138,000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210144 | 9/9 | Homo sapiens | HEK293 |
(d)(U)C Chitosan-based |
Kumar, Awanit | 2021 | 63% | |
Study summaryFull title
All authors
Awanit Kumar, Surendar Reddy Dhadi, Ngoc‐Nu Mai, Catherine Taylor, Jeremy W. Roy, David A. Barnett, Stephen M. Lewis, Anirban Ghosh, and Rodney J. Ouellette
Journal
J Extracell Vesicles
Abstract
Several studies have demonstrated the potential uses of extracellular vesicles (EVs) for liquid biop (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Chitosan-based Protein markers
EV: CD63/ Flotillin1/ CD9/ HSC70
non-EV: CANX Proteomics
yes
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell count
7.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Other
Name other separation method
Chitosan-based
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ HSC70/ CD9/ CD63
Not detected contaminants
CANX
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
133.0 +/- 5.2
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.02E+08
EM
EM-type
Transmission-EM
Image type
Close-up
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EV210089 | 1/2 | Homo sapiens | Blood plasma |
(d)(U)C qEV |
Sabbagh, Quentin | 2021 | 63% | |
Study summaryFull title
All authors
Quentin Sabbagh, Gwennan André-Grégoire, Carolina Alves-Nicolau, Aurélien Dupont, Nicolas Bidère, Emmanuel Jouglar, Laëtitia Guével, Jean-Sébastien Frénel, Julie Gavard
Journal
Sci Rep
Abstract
Glioblastoma is a devastating tumor of the central nervous system characterized by a poor survival a (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD63/ CD9
non-EV: GM130 Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
MLA-130
Pelleting: speed (g)
100000
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Not detected contaminants
GM130
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD9/ CD63
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
Report size (nm)
60
Report type
Not Reported
EV-concentration
Yes
|
||||||||
EV200181 | 2/3 | Homo sapiens | Blood plasma | SEC (non-commercial) | Dlugolecka, Magdalena | 2021 | 63% | |
Study summaryFull title
All authors
Magdalena Dlugolecka, Jacek Szymanski, Lukasz Zareba, Zuzanna Homoncik, Joanna Domagala-Kulawik, Malgorzata Polubiec-Kownacka, Malgorzata Czystowska-Kuzmicz
Journal
Cells
Abstract
The current lack of reliable methods for quantifying extracellular vesicles (EVs) isolated from comp (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
lung cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Size-exclusion chromatography (non-commercial)
Protein markers
EV: TSG101/ CD63/ CD81/ PD-L1/ Syntenin/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ Syntenin/ PD-L1/ TSG101/ CD81
Not detected contaminants
Calnexin
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Fluorescent NTA
Relevant measurements variables specified?
NA
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD9
Not detected EV-associated proteins
CD63
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
98.4
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 2.44E+11
Particle analysis: flow cytometry
Flow cytometer type
BD FACSVerse 8 color Flow Cytometer (BD)
Hardware adjustment
Calibration bead size
4.5
Report type
Not Reported
EM
EM-type
Cryo-EM
Image type
Close-up
|
||||||||
EV200157 | 4/10 | Homo sapiens | MDA-MB-468 |
Polymer-based precipitation SEC (non-commercial) UF |
Martínez-Greene, Juan A | 2021 | 63% | |
Study summaryFull title
All authors
Juan A Martínez-Greene, Karina Hernández-Ortega, Ricardo Quiroz-Baez, Osbaldo Resendis-Antonio, Israel Pichardo-Casas, David A Sinclair, Bogdan Budnik, Alfredo Hidalgo-Miranda, Eileen Uribe-Querol, María Del Pilar Ramos-Godínez, Eduardo Martínez-Martínez
Journal
J Extracell Vesicles
Abstract
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Polymer-based precipitation
Size-exclusion chromatography (non-commercial) Ultrafiltration Protein markers
EV: CD9/ CD81/ ANXA2/ TSG101
non-EV: Calnexin/ Albumin Proteomics
no
Show all info
Study aim
New methodological development/Biomarker/Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MDA-MB-468
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
95
Cell count
1.50E+06
Separation Method
Ultra filtration
Cut-off size (kDa)
3
Membrane type
Regenerated cellulose
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
1
Resin type
Sepharose CL-2B
Other
Name other separation method
Polymer-based precipitation
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD81/ ANXA2/ TSG101
Detected contaminants
Albumin
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
204.17
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 4.68E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200100 | 1/1 | Homo sapiens | colostrum |
(d)(U)C ExoQuick |
Civra, Andrea; Francese, Rache | 2021 | 63% | |
Study summaryFull title
All authors
Andrea Civra, Rachele Francese, Manuela Donalisio, Paola Tonetto, Alessandra Coscia, Stefano Sottemano, Raffaella Balestrini, Antonella Faccio, Laura Cavallarin, Guido E Moro, Enrico Bertino, David Lembo
Journal
J hum Lact
Abstract
Background: It is known that breastfeeding protects the infant from enteric and respiratory infectio (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
colostrum
Sample origin
preterm mothers
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD81/ CD63/ CD9
non-EV: calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
colostrum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
258
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 7.40E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200093 | 10/10 | Homo sapiens | Blood plasma |
(d)(U)C Filtration DG |
Dong, Liang | 2021 | 63% | |
Study summaryFull title
All authors
Liang Dong, Richard C. Zieren, Kengo Horie, Chi‐Ju Kim, Emily Mallick, Yuezhou Jing, Mingxiao Feng, Morgan D. Kuczler, Jordan Green, Sarah R. Amend, Kenneth W. Witwer, Theo M. de Reijke, Yoon‐Kyoung Cho, Kenneth J. Pienta, Wei Xue
Journal
J Extracell Vesicles
Abstract
One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the l (show more...)
EV-METRIC
63% (91st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration DG Protein markers
EV: CD81/ Flotillin1
non-EV: ApoA1 Proteomics
no
EV density (g/ml)
1.10-1.15
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
38
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
230
Fraction volume (mL)
4.75
Fraction processing
Centrifugation
Pelleting: volume per fraction
28
Pelleting: duration (min)
60
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
120000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD81
Not detected contaminants
ApoA1
Flow cytometry
Type of Flow cytometry
NanoFCM
Hardware adaptation to ~100nm EV's
Please refer to the publication below: Zhu S, Ma L, Wang S, et al. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanopar
Calibration bead size
0.2
Antibody details provided?
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
Particle analysis: flow cytometry
Flow cytometer type
NanoFCM
Hardware adjustment
Please refer to the publication below: Zhu S, Ma L, Wang S, et al. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS nano. 2014 Oct 28;8(10):10998-1006.
Calibration bead size
0.2
Report type
Not Reported
|
||||||||
EV200075 | 2/2 | Tetraselmis chuii | Tetraselmis chuii | Tangential flow filtration | Adamo, Giorgia | 2021 | 63% | |
Study summaryFull title
All authors
Giorgia Adamo, David Fierli, Daniele P Romancino, Sabrina Picciotto, Maria E Barone, Anita Aranyos, Darja Božič, Svenja Morsbach, Samuele Raccosta, Christopher Stanly, Carolina Paganini, Meiyu Gai, Antonella Cusimano, Vincenzo Martorana, Rosina Noto, Rita Carrotta, Fabio Librizzi, Loredana Randazzo, Rachel Parkes, Umberto Capasso Palmiero, Estella Rao, Angela Paterna, Pamela Santonicola, Ales Iglič, Laura Corcuera, Annamaria Kisslinger, Elia Di Schiavi, Giovanna L Liguori 10 , Katharina Landfester, Veronika Kralj-Iglič, Paolo Arosio, Gabriella Pocsfalvi, Nicolas Touzet, Mauro Manno, Antonella Bongiovanni
Journal
J Extracell Vesicles
Abstract
Cellular, inter-organismal and cross kingdom communication via extracellular vesicles (EVs) is inten (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small Extracellular Vesicles - Algosomes
Separation protocol
Separation protocol
Tangential flow filtration
Protein markers
EV: Alix/ H/ ATPase/ enolase/ beta-actin/ HSP70
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/novel EV type
Sample
Species
Tetraselmis chuii
Sample Type
Cell culture supernatant
EV-producing cells
Tetraselmis chuii
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
mg dry weight microalgal biomass
Separation Method
Other
Name other separation method
Tangential flow filtration
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
H/ ATPase/ enolase/ beta-actin/ HSP70/ Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
75
NTA
Report type
Size range/distribution
Reported size (nm)
115+/-5
EV concentration
Yes
Particle yield
per mg dry weight microalgal mass 1.00E+09
EM
EM-type
Atomic force-EM/ Transmission-EM/ Scanning-EM/ Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV190087 | 1/6 | Homo sapiens | Saliva |
(d)(U)C Sonication |
Hiraga, Chiho | 2021 | 63% | |
Study summaryFull title
All authors
Chiho Hiraga, Satoshi Yamamoto, Sadamitsu Hashimoto, Masataka Kasahara, Tamiko Minamisawa, Sachiko Matsumura, Akira Katakura, Yasutomo Yajima, Takeshi Nomura, Kiyotaka Shiba
Journal
Sci Rep
Abstract
Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associa (show more...)
EV-METRIC
63% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
Control condition
Focus vesicles
Other / cell debris
Separation protocol
Separation protocol
(d)(U)C
Sonication Protein markers
EV: Alix/ HSP70/ CD63/ CD9/ CD81
non-EV: Argonaute2 Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
2000
Other
Name other separation method
Sonication
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ HSP70/ CD81
Not detected EV-associated proteins
Alix
Detected contaminants
Argonaute2
Characterization: RNA analysis
RNA analysis
Type
Capillary electrophoresis (e.g. Bioanalyzer);Other
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Atomic force-EM/ Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190087 | 2/6 | Homo sapiens | Saliva |
(d)(U)C Sonication |
Hiraga, Chiho | 2021 | 63% | |
Study summaryFull title
All authors
Chiho Hiraga, Satoshi Yamamoto, Sadamitsu Hashimoto, Masataka Kasahara, Tamiko Minamisawa, Sachiko Matsumura, Akira Katakura, Yasutomo Yajima, Takeshi Nomura, Kiyotaka Shiba
Journal
Sci Rep
Abstract
Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associa (show more...)
EV-METRIC
63% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
Control condition
Focus vesicles
(shedding) microvesicle
Separation protocol
Separation protocol
(d)(U)C
Sonication Protein markers
EV: CD81/ Alix/ CD63/ CD9/ HSP70
non-EV: Argonaute2 Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
10000
Other
Name other separation method
Sonication
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ HSP70
Not detected EV-associated proteins
CD81/ CD9/ Alix
Detected contaminants
Argonaute2
Characterization: RNA analysis
RNA analysis
Type
Capillary electrophoresis (e.g. Bioanalyzer);Other
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
291.3 ± 22.8
EV concentration
Yes
EM
EM-type
Atomic force-EM/ Transmission-EM
Image type
Wide-field
|
||||||||
EV190087 | 3/6 | Homo sapiens | Saliva |
(d)(U)C Sonication |
Hiraga, Chiho | 2021 | 63% | |
Study summaryFull title
All authors
Chiho Hiraga, Satoshi Yamamoto, Sadamitsu Hashimoto, Masataka Kasahara, Tamiko Minamisawa, Sachiko Matsumura, Akira Katakura, Yasutomo Yajima, Takeshi Nomura, Kiyotaka Shiba
Journal
Sci Rep
Abstract
Oral fluids (OFs) contain small extracellular vesicles (sEVs or exosomes) that carry disease-associa (show more...)
EV-METRIC
63% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Sonication Protein markers
EV: Alix/ HSP70/ CD63/ CD9/ CD81
non-EV: Argonaute2 Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Other
Name other separation method
Sonication
EV-subtype
Distinction between multiple subtypes
Used subtypes
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ CD63/ HSP70/ CD81
Detected contaminants
Argonaute2
Characterization: RNA analysis
RNA analysis
Type
Other;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Atomic force-EM/ Transmission-EM
Image type
Wide-field
Report size (nm)
52.7 ± 30.7
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