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You searched for: EV230974 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230974 | 1/1 | Citrus lemon | Juice |
(d)(U)C Filtration UF qEV |
Steć A | 2023 | 17% | |
Study summaryFull title
All authors
Steć A, Chodkowska M, Kasprzyk-Pochopień J, Mielczarek P, Piekoszewski W, Lewczuk B, Płoska A, Kalinowski L, Wielgomas B, Dziomba S
Journal
Food Chem
Abstract
A new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon juice s (show more...)
EV-METRIC
17% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Juice
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration qEV Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
New methodological development
Sample
Species
Citrus lemon
Sample Type
Juice
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
100,000
Membrane type
Polyethersulfone (PES)
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
70
EV concentration
Yes
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EV-TRACK ID | EV230974 |
---|---|
species | Citrus lemon |
sample type | Juice |
condition | Control condition |
separation protocol | dUC/ Filtration/ Ultrafiltration/ qEV |
Exp. nr. | 1 |
EV-METRIC % | 17 |