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You searched for: EV230972 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230972 | 1/5 | Homo sapiens | DKs-8 |
(d)(U)C DC DG |
Jimenez L | 2023 | 67% | |
Study summaryFull title
All authors
Jimenez L, Barman B, Jung YJ, Cocozza L, Krystofiak E, Saffold C, Vickers KC, Wilson JT, Dawson TR, Weaver AM
Journal
J Extracell Vesicles
Abstract
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Adj. k-factor
3.13 (washing)
Protein markers
EV: CD63/ Flotillin-1
non-EV: Argonaute-2 Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DKs-8
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Cell count
224000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
3
Wash: time (min)
30
Wash: Rotor Type
TLA-110
Wash: speed (g)
10000
Wash: adjusted k-factor
3.13E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
3.130
Density cushion
Density medium
Iodixanol
Sample volume
30
Cushion volume
2
Density of the cushion
60%
Centrifugation time
240
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotillin-1
Detected contaminants
Argonaute-2
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase cocktail
RNAse concentration
5/ 200U/µL
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
145
EV concentration
Yes
Particle yield
as number of particles per cell per hour: 2
|
||||||||
EV230972 | 2/5 | Homo sapiens | DKs-8 |
(d)(U)C DC DG |
Jimenez L | 2023 | 67% | |
Study summaryFull title
All authors
Jimenez L, Barman B, Jung YJ, Cocozza L, Krystofiak E, Saffold C, Vickers KC, Wilson JT, Dawson TR, Weaver AM
Journal
J Extracell Vesicles
Abstract
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Opti-MEM
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Adj. k-factor
3.13 (washing)
Protein markers
EV: CD63/ Flotillin-1
non-EV: Argonaute-2 Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DKs-8
EV-harvesting Medium
Serum free medium
Cell viability (%)
98
Cell count
234000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
3
Wash: time (min)
30
Wash: Rotor Type
TLA-110
Wash: speed (g)
10000
Wash: adjusted k-factor
3.13E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
3.130
Density cushion
Density medium
Iodixanol
Sample volume
30
Cushion volume
2
Density of the cushion
60%
Centrifugation time
240
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotillin-1
Detected contaminants
Argonaute-2
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase cocktail
RNAse concentration
5/ 200U/µL
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
145
EV concentration
Yes
Particle yield
as number of particles per cell per hour: 1.7
|
||||||||
EV230972 | 3/5 | Homo sapiens | DKs-8 |
(d)(U)C DC DG |
Jimenez L | 2023 | 67% | |
Study summaryFull title
All authors
Jimenez L, Barman B, Jung YJ, Cocozza L, Krystofiak E, Saffold C, Vickers KC, Wilson JT, Dawson TR, Weaver AM
Journal
J Extracell Vesicles
Abstract
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Adj. k-factor
3.13 (washing)
Protein markers
EV: CD63/ Flotillin-1
non-EV: Albumin/ Argonaute-2 Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DKs-8
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
98
Cell count
250800000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
3
Wash: time (min)
30
Wash: Rotor Type
TLA-110
Wash: speed (g)
10000
Wash: adjusted k-factor
3.13E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
3.130
Density cushion
Density medium
Iodixanol
Sample volume
30
Cushion volume
2
Density of the cushion
60%
Centrifugation time
240
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotillin-1
Detected contaminants
Albumin/ Argonaute-2
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase cocktail
RNAse concentration
5/ 200U/µL
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
145
EV concentration
Yes
Particle yield
as number of particles per cell per hour: 1.9
|
||||||||
EV230972 | 4/5 | Homo sapiens | DLD-1 |
(d)(U)C DC DG |
Jimenez L | 2023 | 67% | |
Study summaryFull title
All authors
Jimenez L, Barman B, Jung YJ, Cocozza L, Krystofiak E, Saffold C, Vickers KC, Wilson JT, Dawson TR, Weaver AM
Journal
J Extracell Vesicles
Abstract
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Adj. k-factor
3.13 (washing)
Protein markers
EV: CD63/ Flotillin-1
non-EV: Argonaute-2 Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DLD-1
EV-harvesting Medium
Serum free medium
Cell viability (%)
98
Cell count
254000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
3
Wash: time (min)
30
Wash: Rotor Type
TLA-110
Wash: speed (g)
10000
Wash: adjusted k-factor
3.13E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
3.130
Density cushion
Density medium
Iodixanol
Sample volume
30
Cushion volume
2
Density of the cushion
60%
Centrifugation time
240
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotillin-1
Detected contaminants
Argonaute-2
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase cocktail
RNAse concentration
5/ 200U/µL
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
145
EV concentration
Yes
Particle yield
as number of particles per cell per hour: 2
|
||||||||
EV230972 | 5/5 | Homo sapiens | DLD-1 |
(d)(U)C DC DG |
Jimenez L | 2023 | 67% | |
Study summaryFull title
All authors
Jimenez L, Barman B, Jung YJ, Cocozza L, Krystofiak E, Saffold C, Vickers KC, Wilson JT, Dawson TR, Weaver AM
Journal
J Extracell Vesicles
Abstract
Extracellular vesicle (EV)-carried miRNAs can influence gene expression and functional phenotypes in (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density cushion Density gradient Adj. k-factor
3.13 (washing)
Protein markers
EV: CD63/ Flotillin-1
non-EV: Albumin/ Argonaute-2 Proteomics
no
EV density (g/ml)
1.14
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DLD-1
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell viability (%)
98
Cell count
274000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
10000
Wash: volume per pellet (ml)
3
Wash: time (min)
30
Wash: Rotor Type
TLA-110
Wash: speed (g)
10000
Wash: adjusted k-factor
3.13E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
1
Orientation
Bottom-up
Speed (g)
100000
Duration (min)
1200
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
3
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
3.130
Density cushion
Density medium
Iodixanol
Sample volume
30
Cushion volume
2
Density of the cushion
60%
Centrifugation time
240
Centrifugation speed
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotillin-1
Detected contaminants
Albumin/ Argonaute-2
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
Yes
RNAse type
RNase cocktail
RNAse concentration
5/ 200U/µL
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
145
EV concentration
Yes
Particle yield
as number of particles per cell per hour: 2
|
||||||||
1 - 5 of 5 |
EV-TRACK ID | EV230972 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | ||||
cell type | DKs-8 | DKs-8 | DKs-8 | DLD-1 | DLD-1 |
medium | Serum free medium | Serum free medium | EV-depleted medium | Serum free medium | EV-depleted medium |
condition | Control condition | Opti-MEM | Control condition | Control condition | Control condition |
separation protocol | dUC/ DC/ Density gradient | dUC/ DC/ Density gradient | dUC/ DC/ Density gradient | dUC/ DC/ Density gradient | dUC/ DC/ Density gradient |
Exp. nr. | 1 | 2 | 3 | 4 | 5 |
EV-METRIC % | 67 | 67 | 67 | 67 | 67 |