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You searched for: EV230962 (EV-TRACK ID)
Showing 1 - 7 of 7
Showing 1 - 7 of 7
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230962 | 1/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 67% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
67% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD63/ CD81/ Flotillin-1/ TSG101/ ANXA5/ EpCAM/ ICAM1
non-EV: GM130 Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Detected EV-associated proteins
Alix/ CD63/ CD81/ Flotillin-1/ TSG101/ ANXA5/ EpCAM/ ICAM1
Not detected EV-associated proteins
EpCAM
Not detected contaminants
GM130
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
127
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.01E+10
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
|
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EV230962 | 2/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Low-temperature treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
106
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 3.60E+09
|
||||||||
EV230962 | 3/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Homogenized
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
113
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.06E+08
|
||||||||
EV230962 | 4/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
High-temperature pasteurized
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
115
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.20E+09
|
||||||||
EV230962 | 5/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Ultra-high-temperature pasteurized
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
121
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 3.15E+09
|
||||||||
EV230962 | 6/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Homogenized + high-temperature pasteurized
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
118
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.65E+09
|
||||||||
EV230962 | 7/6 | Bos bovis | bovine milk | (d)(U)C | Colella, Anna P. | 2023 | 14% | |
Study summaryFull title
All authors
Anna P. Colella, Anuradha Prakash, John J. Miklavcic
Journal
Food Science and Nutrition
Abstract
Extracellular vesicles (EVs) in bovine milk confer beneficial physiologic effects to consumers. Indu (show more...)
EV-METRIC
14% (37th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
bovine milk
Sample origin
Homogenized + ultra-high-temperature pasteurized
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Bos bovis
Sample Type
bovine milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
130000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: RNA analysis
RNA analysis
Type
fluorometric for total miRNA
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
147
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.10E+09
|
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1 - 7 of 7 |
EV-TRACK ID | EV230962 | ||||||
---|---|---|---|---|---|---|---|
species | Bos bovis | ||||||
sample type | bovine milk | ||||||
condition | Control condition | Low-temperature treated | Homogenized | High-temperature pasteurized | Ultra-high-temperature pasteurized | Homogenized high-temperature pasteurized | Homogenized ultra-high-temperature pasteurized |
separation protocol | dUC | dUC | dUC | dUC | dUC | dUC | dUC |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
EV-METRIC % | 67 | 14 | 14 | 14 | 14 | 14 | 14 |