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You searched for: EV230700 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230700 | 1/2 | Vibrio mediterranei | AK1 |
(d)(U)C DG Filtration UF |
Li J | 2016 | 57% | |
Study summaryFull title
All authors
Li J, Azam F, Zhang S
Journal
Environ Microbiol
Abstract
Production and release of outer-membrane vesicles (OMVs) is known in many bacteria including human p (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Grown at 20°C
Focus vesicles
outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Function
Sample
Species
Vibrio mediterranei
Sample Type
Cell culture supernatant
EV-producing cells
AK1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
P27A
Pelleting: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
9
Lowest density fraction
0%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
not specified
Sample volume (mL)
1.3
Orientation
Bottom-up
Speed (g)
111132
Duration (min)
360
Fraction volume (mL)
not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
not spec
Pelleting: rotor type
P90AT
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per 4 l of starting sample
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
20-120
|
||||||||
EV230700 | 2/2 | Vibrio mediterranei | AK1 |
(d)(U)C DG Filtration UF |
Li J | 2016 | 57% | |
Study summaryFull title
All authors
Li J, Azam F, Zhang S
Journal
Environ Microbiol
Abstract
Production and release of outer-membrane vesicles (OMVs) is known in many bacteria including human p (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Grown at 30°C
Focus vesicles
outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Ultrafiltration Protein markers
EV: None
non-EV: None Proteomics
yes
EV density (g/ml)
not specified
Show all info
Study aim
Function
Sample
Species
Vibrio mediterranei
Sample Type
Cell culture supernatant
EV-producing cells
AK1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
P27A
Pelleting: speed (g)
100000
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
9
Lowest density fraction
0%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
not specified
Sample volume (mL)
1.3
Orientation
Bottom-up
Speed (g)
111132
Duration (min)
360
Fraction volume (mL)
not specified
Fraction processing
Centrifugation
Pelleting: volume per fraction
not spec
Pelleting: rotor type
P90AT
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
TDB
Filtration steps
0.2 or 0.22 µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per 4 l of starting sample
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
20-120
|
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1 - 2 of 2 |
EV-TRACK ID | EV230700 | |
---|---|---|
species | Vibrio mediterranei | |
sample type | Cell culture | |
cell type | AK1 |