Search > Results
You searched for: EV230666 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230666 | 1/5 | Aggregatibacter actinomycetemcomitans | Strain 173 |
(d)(U)C DG Filtration |
Kieselbach T | 2015 | 33% | |
Study summaryFull title
All authors
Kieselbach T, Zijnge V, Granström E, Oscarsson J
Journal
PLoS One
Abstract
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive fo (show more...)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
1 (pelleting) / 1 (washing)
Protein markers
EV: GroEL
non-EV: None Proteomics
yes
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
Strain 173
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85000
Pelleting: adjusted k-factor
1,52E
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
85000
Wash: adjusted k-factor
1,52E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4.170
Sample volume (mL)
0.150
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
9,55E
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Spectrophotometry
Western Blot
Detected EV-associated proteins
GroEL
Proteomics database
ProteomeXchange
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Atomic force microscopy
Image type
Wide-field
|
||||||||
EV230666 | 3/5 | Aggregatibacter actinomycetemcomitans | D7SS |
(d)(U)C DG Filtration |
Kieselbach T | 2015 | 29% | |
Study summaryFull title
All authors
Kieselbach T, Zijnge V, Granström E, Oscarsson J
Journal
PLoS One
Abstract
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive fo (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
delltxA delcdtABC
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
1 (pelleting) / 1 (washing)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
D7SS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85000
Pelleting: adjusted k-factor
1,52E
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
85000
Wash: adjusted k-factor
1,52E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4.170
Sample volume (mL)
0.150
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
9,55E
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Spectrophotometry
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230666 | 4/5 | Aggregatibacter actinomycetemcomitans | D7SS |
(d)(U)C DG Filtration |
Kieselbach T | 2015 | 29% | |
Study summaryFull title
All authors
Kieselbach T, Zijnge V, Granström E, Oscarsson J
Journal
PLoS One
Abstract
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive fo (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
delpal
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
1 (pelleting) / 1 (washing)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
D7SS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85000
Pelleting: adjusted k-factor
1,52E
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
85000
Wash: adjusted k-factor
1,52E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4.170
Sample volume (mL)
0.150
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
9,55E
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Spectrophotometry
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230666 | 5/5 | Aggregatibacter actinomycetemcomitans | JP2 |
(d)(U)C DG Filtration |
Kieselbach T | 2015 | 29% | |
Study summaryFull title
All authors
Kieselbach T, Zijnge V, Granström E, Oscarsson J
Journal
PLoS One
Abstract
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive fo (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
1 (pelleting) / 1 (washing)
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
JP2
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85000
Pelleting: adjusted k-factor
1,52E
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
85000
Wash: adjusted k-factor
1,52E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4.170
Sample volume (mL)
0.150
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
9,55E
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
None
Protein Concentration Method
Spectrophotometry
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230666 | 2/5 | Aggregatibacter actinomycetemcomitans | D7SS |
(d)(U)C DG Filtration |
Kieselbach T | 2015 | 22% | |
Study summaryFull title
All authors
Kieselbach T, Zijnge V, Granström E, Oscarsson J
Journal
PLoS One
Abstract
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive fo (show more...)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Density gradient Filtration Adj. k-factor
1 (pelleting) / 1 (washing)
Protein markers
EV: GroEL
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Aggregatibacter actinomycetemcomitans
Sample Type
Cell culture supernatant
EV-producing cells
D7SS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
85000
Pelleting: adjusted k-factor
1,52E
Wash: time (min)
120
Wash: Rotor Type
Type 70 Ti
Wash: speed (g)
85000
Wash: adjusted k-factor
1,52E
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
7
Lowest density fraction
10%
Highest density fraction
45%
Total gradient volume, incl. sample (mL)
4.170
Sample volume (mL)
0.150
Orientation
Bottom-up
Speed (g)
180000
Duration (min)
180
Fraction volume (mL)
0.2
Fraction processing
Centrifugation
Pelleting: speed (g)
16000
Pelleting: adjusted k-factor
9,55E
Pelleting-wash: speed (g)
JA-18.1
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Spectrophotometry
Western Blot
Detected EV-associated proteins
GroEL
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
1 - 5 of 5 |
EV-TRACK ID | EV230666 | ||||
---|---|---|---|---|---|
species | Aggregatibacter actinomycetemcomitans | ||||
sample type | Cell culture | ||||
cell type | Strain 173 | D7SS | D7SS | JP2 | D7SS |
condition | Control condition | delltxA delcdtABC | delpal | Control condition | Control condition |
separation protocol | dUC/ Density gradient/ Filtration | dUC/ Density gradient/ Filtration | dUC/ Density gradient/ Filtration | dUC/ Density gradient/ Filtration | dUC/ Density gradient/ Filtration |
Exp. nr. | 1 | 3 | 4 | 5 | 2 |
EV-METRIC % | 33 | 29 | 29 | 29 | 22 |