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You searched for: EV230656 (EV-TRACK ID)

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Results list Study Tree
Experiment number
  • If needed, multiple experiments were identified in a single publication based on differing sample types, separation protocols and/or vesicle types of interest.
Species
  • Species of origin of the EVs.
Separation protocol
  • Gives a short, non-chronological overview of the different steps of the separation protocol.
    • (d)(U)C = (differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
Details EV-TRACK ID Experiment nr. Species Sample type Separation protocol First author Year EV-METRIC
EV230656 3/21 Vibrio cholerae C6706 NA Rompikuntal PK 2015 38%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
NA
Protein markers
EV: PrtV/ OmpU/ none
non-EV: Crp/ none/ nonen
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
Other
Name other separation method
NA
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
PrtV/ OmpU
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
Crp
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
nonen
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
105
EV concentration
Yes
EM
EM-type
Immuno-­EM
Image type
Wide-field
EV230656 5/21 Vibrio cholerae C6706 NA Rompikuntal PK 2015 38%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
delprtV
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
NA
Protein markers
EV: OmpU/ PrtV/ none
non-EV: Crp/ none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
Other
Name other separation method
NA
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Detected EV-associated proteins
OmpU
Not detected EV-associated proteins
PrtV
Detected contaminants
none
Not detected contaminants
Crp
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
105
EV concentration
Yes
EM
EM-type
Immuno-­EM
Image type
Wide-field
EV230656 2/21 Vibrio cholerae C6706 (d)(U)C
Filtration 		Rompikuntal PK 2015 33%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: OmpU/ PrtV/ none
non-EV: Crp/ none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
OmpU/ PrtV
Not detected contaminants
Crp
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 4/21 Vibrio cholerae C6706 (d)(U)C
Filtration 		Rompikuntal PK 2015 33%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
delprtV
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: OmpU/ PrtV/ none
non-EV: Crp/ none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
OmpU
Not detected EV-associated proteins
PrtV
Detected contaminants
none
Not detected contaminants
Crp
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 8/21 Vibrio cholerae C6706 (d)(U)C
Filtration 		Rompikuntal PK 2015 33%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
33% (74th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
delpkd
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: OmpU/ PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
OmpU
Not detected EV-associated proteins
PrtV
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 1/21 Vibrio cholerae A1552 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
A1552
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 6/21 Vibrio cholerae C6706 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
deltolC
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 7/21 Vibrio cholerae C6706 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
delhlyD
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 9/21 Vibrio cholerae P27459 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
P27459
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 10/21 Vibrio cholerae 569B (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
569B
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 1
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 11/21 Vibrio cholerae 3083 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
3083
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 12/21 Vibrio cholerae 3083 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
delepsC
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: none/ PrtV
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
3083
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
none
Not detected EV-associated proteins
PrtV
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 13/21 Vibrio cholerae V52 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: none/ PrtV
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
V52
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
none
Not detected EV-associated proteins
PrtV
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 14/21 Vibrio cholerae V:5/04 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrTV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
V:5/04
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrTV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 15/21 Vibrio cholerae V:6/04 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
V:6/04
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 16/21 Vibrio cholerae 93Ag19 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
93Ag19
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 17/21 Vibrio cholerae NAGV6 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
NAGV6
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 18/21 Vibrio cholerae KI17036 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
KI17036
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 19/21 Vibrio cholerae AJ-2 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PtrV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
AJ-2
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PtrV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 20/21 Vibrio cholerae AJ-3 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
AJ-3
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Other 2
SDS-PAGE
Detected EV-associated proteins
none
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EV230656 21/21 Vibrio cholerae AJ-4 (d)(U)C
Filtration 		Rompikuntal PK 2015 22%

Study summary

Full title
Outer Membrane Vesicle-Mediated Export of Processed PrtV Protease from Vibrio cholerae.
All authors
Rompikuntal PK, Vdovikova S, Duperthuy M, Johnson TL, Åhlund M, Lundmark R, Oscarsson J, Sandkvist M, Uhlin BE, Wai SN
Journal
PLoS One
Abstract
Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during no (show more...)Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that OMVs may therefore be able to transport bacterial proteases into the target host cells. We present here an analysis of the Vibrio cholerae OMV-associated protease PrtV. (hide)
EV-METRIC
22% (58th percentile of all experiments on the same sample type)
 Reported
 Not reported
 Not applicable
EV-enriched proteins
Protein analysis: analysis of three or more EV-enriched proteins
non EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody specifics
Protein analysis: antibody clone/reference number and dilution
lysate preparation
Protein analysis: lysis buffer composition
Study data
Sample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vesicle (OMV)
Separation protocol
Separation protocol
  • Gives a short, non-chronological overview of the
    different steps of the separation protocol.
    • dUC = (Differential) (ultra)centrifugation
    • DG = density gradient
    • UF = ultrafiltration
    • SEC = size-exclusion chromatography
    • IAF = immuno-affinity capture
(Differential) (ultra)centrifugation
Filtration
Protein markers
EV: PrtV/ none
non-EV: none
Proteomics
no
Show all info
Study aim
Function/Biogenesis/cargo sorting
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
AJ-4
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
PrtV
Not detected EV-associated proteins
none
Detected contaminants
none
Not detected contaminants
none
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
1 - 21 of 21
  • CM = Commercial method
  • dUC = differential ultracentrifugation
  • DG = density gradient
  • UF = ultrafiltration
  • SEC = size-exclusion chromatography
EV-TRACK ID
EV230656
species
Vibrio
cholerae
sample type
Cell
culture
cell type
C6706
C6706
C6706
C6706
C6706
A1552
C6706
C6706
P27459
569B
3083
3083
V52
V:5/04
V:6/04
93Ag19
NAGV6
KI17036
AJ-2
AJ-3
AJ-4
condition
Control
condition
delprtV
Control
condition
delprtV
delpkd
Control
condition
deltolC
delhlyD
Control
condition
Control
condition
Control
condition
delepsC
Control
condition
Control
condition
Control
condition
Control
condition
Control
condition
Control
condition
Control
condition
Control
condition
Control
condition
separation protocol
NA
NA
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
dUC/
Filtration
Exp. nr.
3
5
2
4
8
1
6
7
9
10
11
12
13
14
15
16
17
18
19
20
21
EV-METRIC %
38
38
33
33
33
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22
22