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You searched for: EV230583 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230583 | 1/3 | Homo sapiens | Human neocortical neurons |
Filtration SEC (non-commercial) |
Anastasi F | 2021 | 17% | |
Study summaryFull title
All authors
Anastasi F, Masciandaro SM, Carratore RD, Dell'Anno MT, Signore G, Falleni A, McDonnell LA, Bongioanni P
Journal
Int J Mol Sci
Abstract
Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegener (show more...)
EV-METRIC
17% (55th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Neuroepithelial stem cells
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Size-exclusion chromatography (non-commercial) Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Human neocortical neurons
EV-harvesting Medium
Serum free medium
Separation Method
Filtration steps
0.2 or 0.22 µm
Size-exclusion chromatography
Total column volume (mL)
24
Sample volume/column (mL)
0.2
Resin type
Superose 6
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange Consortium
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
70.5
|
||||||||
EV230583 | 2/3 | Homo sapiens | Blood plasma | IAF | Anastasi F | 2021 | 17% | |
Study summaryFull title
All authors
Anastasi F, Masciandaro SM, Carratore RD, Dell'Anno MT, Signore G, Falleni A, McDonnell LA, Bongioanni P
Journal
Int J Mol Sci
Abstract
Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegener (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Size-exclusion chromatography
Resin type
Immunoaffinity capture
Selected surface protein(s)
L1CAM
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange Consortium
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
115
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230583 | 3/3 | Homo sapiens | Blood plasma | IAF | Anastasi F | 2021 | 17% | |
Study summaryFull title
All authors
Anastasi F, Masciandaro SM, Carratore RD, Dell'Anno MT, Signore G, Falleni A, McDonnell LA, Bongioanni P
Journal
Int J Mol Sci
Abstract
Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegener (show more...)
EV-METRIC
17% (46th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Parkinson's disease patients
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
L1CAM
Characterization: Protein analysis
Protein Concentration Method
Not determined
Proteomics database
ProteomeXchange Consortium
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
97
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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1 - 3 of 3 |
EV-TRACK ID | EV230583 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Blood plasma | Blood plasma |
cell type | Human neocortical neurons | NA | NA |
medium | Serum free medium | NA | NA |
condition | Neuroepithelial stem cells | Control condition | Parkinson's disease patients |
separation protocol | Filtration/ Size-exclusion chromatography (non-commercial) | IAF capture (non-commercial) | IAF capture (non-commercial) |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 17 | 17 | 17 |