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You searched for: EV230062 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230062 | 5/6 | Mus musculus | Blood plasma |
(d)(U)C Filtration |
Choi YY | 2023 | 63% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ CD63/ CD81/ CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ CD45/ CD41
non-EV: Calnexin/ GM130 Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
Not reported
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected contaminants
Calnexin/ GM130
Flow cytometry
Type of Flow cytometry
conventional flow cytometry
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ CD45/ CD41
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ RNA -sequencing
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
85
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~35000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230062 | 1/6 | Homo sapiens | HUVEC/THP1 (coculture) |
(d)(U)C Filtration UF |
Choi YY | 2023 | 50% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
50% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Protein markers
EV: CD31/ CD105/ CD144/ CD146
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HUVEC/THP1 (coculture)
EV-harvesting Medium
Serum-containing medium
Cell viability (%)
90
Cell count
4000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Flow cytometry
Type of Flow cytometry
conventional flow cytometer
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
119
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~2.5E06
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV230062 | 2/6 | Homo sapiens | HUVEC/THP1 (coculture) |
(d)(U)C Filtration UF |
Choi YY | 2023 | 50% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
50% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Irradiation of co-culture (1 Gy)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Ultrafiltration Protein markers
EV: CD31/ CD105/ CD144/ CD146
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HUVEC/THP1 (coculture)
EV-harvesting Medium
Serum-containing medium
Cell viability (%)
90
Cell count
4000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Flow cytometry
Type of Flow cytometry
conventional flow cytometry
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
112
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~5E06
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230062 | 3/6 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Choi YY | 2023 | 50% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
50% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ HLA-DR/ CD45/ CD41
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
Not reported
Flow cytometry
Type of Flow cytometry
conventional flow cytometry
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ HLA-DR/ CD45/ CD41
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
82
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~32000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230062 | 4/6 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Choi YY | 2023 | 50% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
50% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Irradiation of blood sample (1 Gy)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ HLA-DR/ CD45/ CD41
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
Not reported
Flow cytometry
Type of Flow cytometry
conventional flow cytometry
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ HLA-DR/ CD45/ CD41
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
88
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~34000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230062 | 6/6 | Mus musculus | Blood plasma |
(d)(U)C Filtration |
Choi YY | 2023 | 50% | |
Study summaryFull title
All authors
Choi YY, Kim A, Lee Y, Lee YH, Park M, Shin E, Park S, Youn B, Seong KM
Journal
J Extracell Vesicles
Abstract
People exposed to radiation in cancer therapy and nuclear accidents are at increased risk of cardiov (show more...)
EV-METRIC
50% (85th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Irradiation of whole body (1 Gy)
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ CD45/ CD41
non-EV: None Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 µm
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
Not reported
Flow cytometry
Type of Flow cytometry
conventional flow cytometry
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
Antibody details provided?
No
Not detected EV-associated proteins
CD31/ CD105/ CD144/ CD146/ CD14/ CD11b/ CD45/ CD41
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ RNA -sequencing
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
71
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
conventional flow cytometry
Hardware adjustment
Calibration bead size
0.22/ 0.45/ 0.88/ 1.35
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: ~39000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV230062 | |||||
---|---|---|---|---|---|---|
species | Mus musculus | Homo sapiens | Homo sapiens | Homo sapiens | Homo sapiens | Mus musculus |
sample type | Blood plasma | Cell culture | Cell culture | Blood plasma | Blood plasma | Blood plasma |
cell type | NA | HUVEC/THP1 (coculture) | HUVEC/THP1 (coculture) | NA | NA | NA |
medium | NA | Serum-containing medium | Serum-containing medium | NA | NA | NA |
condition | Control condition | Control condition | Irradiation of co-culture (1 Gy) | Control condition | Irradiation of blood sample (1 Gy) | Irradiation of whole body (1 Gy) |
separation protocol | dUC/ Filtration | dUC/ Filtration/ Ultrafiltration | dUC/ Filtration/ Ultrafiltration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration |
Exp. nr. | 5 | 1 | 2 | 3 | 4 | 6 |
EV-METRIC % | 63 | 50 | 50 | 50 | 50 | 50 |