Search > Results
You searched for: EV230012 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV230012 | 1/4 | Mus musculus | cardiac mesenchymal stromal cells |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
cardiac mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
3000000
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
>250
EV concentration
Yes
Particle yield
number of particles per million cells: 90000000
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 2/4 | Mus musculus | cardiac mesenchymal stromal cells |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (93rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Heart failure
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
cardiac mesenchymal stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
90
Cell count
3000000
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per million cells: 40000000
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 3/4 | Mus musculus | Cardiac tissue |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cardiac tissue
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cardiac tissue
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per 100mg tissue: 90000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV230012 | 4/4 | Mus musculus | Cardiac tissue |
Filtration UF SEC (non-commercial) |
Caller T | 2024 | 63% | |
Study summaryFull title
All authors
Caller T, Rotem I, Shaihov-Teper O, Lendengolts D, Schary Y, Shai R, Glick-Saar E, Dominissini D, Motiei M, Katzir I, Popovtzer R, Nahmoud M, Boomgarden A, D'Souza-Schorey C, Naftali-Shani N, Leor J
Journal
Circulation
Abstract
Myocardial infarction (MI) and heart failure are associated with an increased incidence of cancer. H (show more...)
EV-METRIC
63% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cardiac tissue
Sample origin
Heart failure
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Filtration
Ultrafiltration Size-exclusion chromatography (non-commercial) Protein markers
EV: CD9/ CD81/ TSG101
non-EV: None Proteomics
no
Show all info
Study aim
Function/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cardiac tissue
Separation Method
Filtration steps
0.8
Ultra filtration
Cut-off size (kDa)
10
Membrane type
Cellulose acetate
Size-exclusion chromatography
Total column volume (mL)
70
Sample volume/column (mL)
10
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD81/ TSG101
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
<250
EV concentration
Yes
Particle yield
number of particles per 100 mg tissue: 190000000
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV230012 | |||
---|---|---|---|---|
species | Mus musculus | |||
sample type | Cell culture | Cell culture | Cardiac tissue | Cardiac tissue |
cell type | cardiac mesenchymal stromal cells | cardiac mesenchymal stromal cells | NA | NA |
medium | Serum free medium | Serum free medium | NA | NA |
condition | Control condition | Heart failure | Control condition | Heart failure |
separation protocol | Filtration/ Ultrafiltration/ Size-exclusion chromatography (non-commercial) | Filtration/ Ultrafiltration/ Size-exclusion chromatography (non-commercial) | Filtration/ Ultrafiltration/ Size-exclusion chromatography (non-commercial) | Filtration/ Ultrafiltration/ Size-exclusion chromatography (non-commercial) |
Exp. nr. | 1 | 2 | 3 | 4 |
EV-METRIC % | 63 | 63 | 63 | 63 |