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You searched for: EV220412 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220412 | 1/1 | Bos taurus | ovary-derived granulosa cells |
(d)(U)C Filtration |
Menjivar, Nico G | 2023 | 67% | |
Study summaryFull title
All authors
Nico G. Menjivar, Ahmed Gad, Samuel Gebremedhn, Soham Ghosh and Dawit Tesfaye
Journal
Front Cell Dev Biol
Abstract
Climate change-induced global warming results in rises in body temperatures above normal physiologic (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ CD81/ TSG101
non-EV: CYCS Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer
Sample
Species
Bos taurus
Sample Type
Cell culture supernatant
EV-producing cells
ovary-derived granulosa cells
EV-harvesting Medium
EV-depleted medium
Cell count
250000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
3.5
Wash: time (min)
70
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
120000
Filtration steps
0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63/ CD81/ TSG101
Not detected contaminants
CYCS
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
137.75
EV concentration
Yes
Particle yield
as numer of particles per mililiter: 1.02E+11
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
137.75
|
||||||||
EV220412 | 2/1 | Bos taurus | ovary-derived granulosa cells |
(d)(U)C Filtration |
Menjivar, Nico G | 2023 | 67% | |
Study summaryFull title
All authors
Nico G. Menjivar, Ahmed Gad, Samuel Gebremedhn, Soham Ghosh and Dawit Tesfaye
Journal
Front Cell Dev Biol
Abstract
Climate change-induced global warming results in rises in body temperatures above normal physiologic (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Heat stress
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ CD81/ TSG101
non-EV: CYCS Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer
Sample
Species
Bos taurus
Sample Type
Cell culture supernatant
EV-producing cells
ovary-derived granulosa cells
EV-harvesting Medium
EV-depleted medium
Cell count
250000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
3.5
Wash: time (min)
70
Wash: Rotor Type
SW 55 Ti
Wash: speed (g)
120000
Filtration steps
0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63/ CD81/ TSG101
Not detected contaminants
CYCS
Characterization: RNA analysis
RNA analysis
Type
(RT)-(q)PCR/ Capillary electrophoresis (e.g. Bioanalyzer)
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
147.95
EV concentration
Yes
Particle yield
as numer of particles per mililiter: 1.51E+11
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
147.95
|
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1 - 2 of 2 |
EV-TRACK ID | EV220412 | |
---|---|---|
species | Bos taurus | |
sample type | Cell culture | |
cell type | ovary-derived granulosa cells | |
condition | Control condition | Heat stress |
separation protocol | dUC/ Filtration | dUC/ Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 67 | 67 |