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You searched for: EV220366 (EV-TRACK ID)
Showing 1 - 11 of 11
Showing 1 - 11 of 11
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220366 | 1/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 67% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD81/ CD86/ CD80/ MHCII/ CD40
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD81/ CD86/ CD80/ MHCII
Not detected EV-associated proteins
CD40
Detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
75-300
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5.50E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV220366 | 3/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 67% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Ionomycin
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD81/ CD86/ CD80/ MHCII/ CD40
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD81/ CD86/ CD80/ MHCII
Not detected EV-associated proteins
CD40
Detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
75-300
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 7.50E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV220366 | 4/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 67% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 634
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD81/ CD86/ CD80/ MHCII/ CD40
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD81/ CD86/ CD80/ MHCII
Not detected EV-associated proteins
CD40
Detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
75-300
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 8.00E+11
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV220366 | 2/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 44% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
44% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
MPLA
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD86/ CD80/ MHCII/ CD40
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ CD86/ CD80/ MHCII/ CD40
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 5/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2E241
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 6/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2F186
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 7/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2H013
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 8/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2G176
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 9/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2G179a
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220366 | 10/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2G179c
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
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EV220366 | 11/11 | Mus musculus | bone marrow-derived cells | (d)(U)C | Sako Y | 2023 | 14% | |
Study summaryFull title
All authors
Sako Y, Sato-Kaneko F, Shukla NM, Yao S, Belsuzarri MM, Chan M, Saito T, Lao FS, Kong H, Puffer M, Messer K, Pu M, Cottam HB, Carson DA, Hayashi T
Journal
ACS Chem Biol
Abstract
Extracellular vesicles (EVs) transfer antigens and immunomodulatory molecules in immunologic synapse (show more...)
EV-METRIC
14% (43rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
compound 2G179g
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow-derived cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Cell count
3.00E+07
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
100,000
Wash: volume per pellet (ml)
30
Wash: time (min)
180
Wash: Rotor Type
SW 28
Wash: speed (g)
100,000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
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1 - 11 of 11 |
EV-TRACK ID | EV220366 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
species | Mus musculus | ||||||||||
sample type | Cell culture | ||||||||||
cell type | bone marrow-derived cells | ||||||||||
condition | Control condition | Ionomycin | compound 634 | MPLA | compound 2E241 | compound 2F186 | compound 2H013 | compound 2G176 | compound 2G179a | compound 2G179c | compound 2G179g |
separation protocol | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC | dUC |
Exp. nr. | 1 | 3 | 4 | 2 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
EV-METRIC % | 67 | 67 | 67 | 44 | 14 | 14 | 14 | 14 | 14 | 14 | 14 |