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You searched for: EV220279 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220279 | 1/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 2/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Thrombin
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 3/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LPS
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
Fibrillarin/ Cytochrome C/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 4/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
H2O2
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected contaminants
Cytochrome C
Not detected contaminants
Fibrillarin/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
||||||||
EV220279 | 5/5 | Homo sapiens | Umbilical cord blood-mesenchymal stem cells | (d)(U)C | Sung DK | 2019 | 44% | |
Study summaryFull title
All authors
Sung DK, Chang YS, Sung SI, Ahn SY, Park WS
Journal
J Clin Med
Abstract
The aim of this study was to determine the optimal preconditioning regimen for the wound healing the (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD81/ CD63/ CD9/ Angiogenin/ Angiopoietin-1/ HGF/ IGFBP-2/ IGFBP-3/ Pentraxin-3/ Serpin E1/ TIMP-1/ Thrombospondin-1/ uPA/ VEGF
non-EV: Cytochrome C/ Fibrillarin/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Umbilical cord blood-mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000rp
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Detected contaminants
Cytochrome C
Not detected contaminants
Fibrillarin/ GM130
ELISA
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
Angiogenin/ Angiopoietin-1/ HGF/ VEGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
30-100
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM/ Scanning-EM
Image type
Close-up
|
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1 - 5 of 5 |
EV-TRACK ID | EV220279 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | ||||
cell type | Umbilical cord blood-mesenchymal stem cells | ||||
condition | Control condition | Thrombin | LPS | H2O2 | Hypoxia |
separation protocol | dUC | dUC | dUC | dUC | dUC |
Exp. nr. | 1 | 2 | 3 | 4 | 5 |
EV-METRIC % | 44 | 44 | 44 | 44 | 44 |