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You searched for: EV220045 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220045 | 4/4 | Homo sapiens | Blood plasma | IAF | Zhao Z | 2016 | 43% | |
Study summaryFull title
All authors
Zhao Z, Yang Y, Zeng Y, He M
Journal
Lab Chip
Abstract
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as (show more...)
EV-METRIC
43% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Ovarian cancer
Focus vesicles
exosome
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: CD24/ EpCAM/ CA-125
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD24/ CA-125/ EpCAM
Characterization: Protein analysis
Protein Concentration Method
Bradford
Fluorescent NTA
Antibody details provided?
Yes
Detected EV-associated proteins
CD24/ EpCAM/ CA-125
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Immuno-EM
EM protein
Other/ EpCAm/ CD9/ CD81/ CD63/ CA-125
Image type
Close-up
EV concentration
Yes
|
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EV220045 | 3/4 | Homo sapiens | Blood plasma | IAF | Zhao Z | 2016 | 29% | |
Study summaryFull title
All authors
Zhao Z, Yang Y, Zeng Y, He M
Journal
Lab Chip
Abstract
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as (show more...)
EV-METRIC
29% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Ovarian cancer
Focus vesicles
exosome
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: CD24/ EpCAM/ CA-125
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD24/ CA-125/ EpCAM
Characterization: Protein analysis
Protein Concentration Method
Bradford
Fluorescent NTA
Antibody details provided?
Yes
Detected EV-associated proteins
CD24/ EpCAM/ CA-125
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
|
||||||||
EV220045 | 2/4 | Homo sapiens | Blood plasma | IAF | Zhao Z | 2016 | 13% | |
Study summaryFull title
All authors
Zhao Z, Yang Y, Zeng Y, He M
Journal
Lab Chip
Abstract
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as (show more...)
EV-METRIC
13% (30th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
Protein markers
EV: EpCAM/ CA-125/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
CD24/ CA-125/ EpCAM
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD9/ EpCAM/ CA-125
Characterization: Lipid analysis
No
EM
EM-type
Immuno-EM
EM protein
Other/ EpCAm/ CD9/ CD81/ CD63/ CA-125
Image type
Close-up
EV concentration
Yes
|
||||||||
EV220045 | 1/4 | Homo sapiens | Blood plasma | (d)(U)C | Zhao Z | 2016 | 11% | |
Study summaryFull title
All authors
Zhao Z, Yang Y, Zeng Y, He M
Journal
Lab Chip
Abstract
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as (show more...)
EV-METRIC
11% (26th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: EpCAM/ CA-125/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
Yes
Detected EV-associated proteins
CD9/ EpCAM/ CA-125
Characterization: Lipid analysis
No
|
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1 - 4 of 4 |
EV-TRACK ID | EV220045 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Blood plasma | |||
condition | Ovarian cancer | Ovarian cancer | Control condition | Control condition |
separation protocol | IAF capture (non-commercial) | IAF capture (non-commercial) | IAF capture (non-commercial) | dUC |
Exp. nr. | 4 | 3 | 2 | 1 |
EV-METRIC % | 43 | 29 | 13 | 11 |