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You searched for: EV210472 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210472 | 1/3 | Homo sapiens | Blood plasma | (d)(U)C | Hu SS | 2015 | 33% | |
Study summaryFull title
All authors
Hu SS, Zhang HG, Zhang QJ, Xiu RJ
Journal
Endocrine
Abstract
NA (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD31/ CD144/ CD51
non-EV: CD42 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Accuri C6
Antibody details provided?
No
Detected EV-associated proteins
CD31/ CD51/ CD144
Not detected contaminants
CD42
Characterization: Lipid analysis
No
|
||||||||
EV210472 | 2/3 | Homo sapiens | Blood plasma | (d)(U)C | Hu SS | 2015 | 33% | |
Study summaryFull title
All authors
Hu SS, Zhang HG, Zhang QJ, Xiu RJ
Journal
Endocrine
Abstract
NA (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
non-obese with hypertension
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD31/ CD144/ CD51
non-EV: CD42 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Accuri C6
Antibody details provided?
No
Detected EV-associated proteins
CD31/ CD51/ CD144
Not detected contaminants
CD42
Characterization: Lipid analysis
No
|
||||||||
EV210472 | 3/3 | Homo sapiens | Blood plasma | (d)(U)C | Hu SS | 2015 | 33% | |
Study summaryFull title
All authors
Hu SS, Zhang HG, Zhang QJ, Xiu RJ
Journal
Endocrine
Abstract
NA (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
obese with hypertension
Focus vesicles
microparticle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD31/ CD144/ CD51
non-EV: CD42 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Characterization: Protein analysis
Protein Concentration Method
Not determined
Flow cytometry
Type of Flow cytometry
Accuri C6
Antibody details provided?
No
Detected EV-associated proteins
CD31/ CD51/ CD144
Not detected contaminants
CD42
Characterization: Lipid analysis
No
|
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1 - 3 of 3 |
EV-TRACK ID | EV210472 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Blood plasma | ||
condition | Control condition | non-obese with hypertension | obese with hypertension |
separation protocol | dUC | dUC | dUC |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 33 | 33 | 33 |