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You searched for: EV210404 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210404 | 1/1 | Homo sapiens | Corneal mesenchymal stem cells |
(d)(U)C UF |
Samaeekia R | 2018 | 44% | |
Study summaryFull title
All authors
Samaeekia R, Rabiee B, Putra I, Shen X, Park YJ, Hematti P, Eslani M, Djalilian AR
Journal
Invest Ophthalmol Vis Sci
Abstract
Mesenchymal stromal cells (MSCs) have been used therapeutically to modulate inflammation and promote (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Protein markers
EV: CD81/ HSP70/ CD63/ CD9
non-EV: Calnexin/ Cytochrome c/ GM130 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Corneal mesenchymal stem cells
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: speed (g)
100000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
NS
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ HSP70/ CD81
Detected contaminants
Calnexin/ GM130
Not detected contaminants
Cytochrome c
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
40-280
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
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1 - 1 of 1 |
EV-TRACK ID | EV210404 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | Corneal mesenchymal stem cells |
condition | Control condition |
separation protocol | dUC/ Ultrafiltration |
Exp. nr. | 1 |
EV-METRIC % | 44 |