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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210402	1/1	Homo sapiens	Follicular fluid	(d)(U)C	Franz C	2016	29%
Study summary Full title Extracellular vesicles in human follicular fluid do not promote coagulation. All authors Franz C, Böing AN, Montag M, Strowitzki T, Markert UR, Mastenbroek S, Nieuwland R, Toth B Journal Reprod Biomed Online Abstract Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an (show more...)Body fluids contain extracellular vesicles expressing tissue factor on their surface and serve as an additional trigger for coagulation. During the menstrual cycle ovarian tissue restoration is mandatory and it is unknown whether follicular fluid might provide procoagulant substances. Within an observational study, follicular fluid from women undergoing IVF/intracytoplasmic sperm injection (ICSI) was analysed by fluorescence-activated cell sorting (FACS), electron microscopy, resistive pulse sensing (RPS), nanoparticle-tracking analysis (NTA) and fibrin generation tests (FGT). The presence of extracellular vesicles, especially CD9-positive extracellular vesicles in follicular fluid, was proven. However, clotting tests revealed no procoagulant properties of the detected extracellular vesicles. (hide) EV-METRIC 29% (39th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Follicular fluid Sample origin Gonadotrophin-releasing hormone agonist Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: HSP70/ CD14/ EGFR/ CD24/ CD45/ L1CAM/ CD9 non-EV: None Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Follicular fluid Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: speed (g) 18890 Wash: volume per pellet (ml) 0.225 Wash: time (min) 30 Wash: speed (g) 18890 Characterization: Protein analysis Protein Concentration Method Not determined Flow cytometry Type of Flow cytometry FACS Calibur (Becton Dickinson) Detected EV-associated proteins HSP70/ CD9/ CD14/ EGFR/ CD24/ CD45/ L1CAM Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Size range/distribution Reported size (nm) 70-1075 EV concentration Yes Particle yield as number of particles per milliliter of starting sample: 2.30e+10 TRPS Report type Size range/distribution Reported size (nm) 165-200 EV concentration Yes Particle yield as number of particles per milliliter of starting sample: 1.20e+8 EM EM-type Transmission-EM Image type Close-up

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EV-TRACK ID	EV210402
species	Homo sapiens
sample type	Follicular fluid
condition	Gonadotrophin-releasing hormone agonist
separation protocol	dUC
Exp. nr.	1
EV-METRIC %	29