EV-TRACK

Search > Results

You searched for: EV210382 (EV-TRACK ID)

Showing 1 - 4 of 4

Results list Study Tree

Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210382	1/4	Homo sapiens	maternal placenta perfusate	(d)(U)C	Awoyemi T	2023	78%
Study summary Full title A cross-sectional analysis of syncytiotrophoblast membrane extracellular vesicles-derived transcriptomic biomarkers in early-onset preeclampsia. All authors Awoyemi T, Zhang W, Rahbar M, Cribbs A, Logenthiran P, Jiang S, Collett G, Cerdeira AS, Vatish M Journal Front Cardiovasc Med Abstract Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies world (show more...)Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies worldwide. Biomarker(s) for the disorder exists, but while these have excellent negative predictive value, their positive predictive value is poor. Extracellular vesicles released by the placenta into the maternal circulation, syncytiotrophoblast membrane extracellular vesicles (STB-EVs), have been identified as being involved in PE with the potential to act as liquid biopsies. (hide) EV-METRIC 78% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type maternal placenta perfusate Sample origin Control condition Focus vesicles medium/large extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: Alix/ PLAP/ CD9 non-EV: Cytochrome C Proteomics yes Show all info Study aim Biomarker/Identification of content (omics approaches) Sample Species Homo sapiens Sample Type maternal placenta perfusate Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: time(min) 30 Pelleting: rotor type Sorvall TST28.39 Pelleting: speed (g) 10,000 Wash: volume per pellet (ml) 45 Wash: time (min) 30 Wash: Rotor Type Sorvall TST28.39 Wash: speed (g) 10,000 Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) 8680-10240 Western Blot Detected EV-associated proteins Alix/ PLAP Not detected EV-associated proteins CD9 Not detected contaminants Cytochrome C Proteomics database PRIDE Characterization: RNA analysis RNA analysis Type (RT)-(q)PCR/ RNA-sequencing/ Capillary electrophoresis (e.g. Bioanalyzer) Database NCBI geo Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Modus Reported size (nm) 205.8 EV concentration Yes Particle yield 6.98E11-1.12E12 EM EM-type Transmission-EM Image type Close-up, Wide-field

	EV210382	2/4	Homo sapiens	maternal placenta perfusate	(d)(U)C Filtration	Awoyemi T	2023	78%
Study summary Full title A cross-sectional analysis of syncytiotrophoblast membrane extracellular vesicles-derived transcriptomic biomarkers in early-onset preeclampsia. All authors Awoyemi T, Zhang W, Rahbar M, Cribbs A, Logenthiran P, Jiang S, Collett G, Cerdeira AS, Vatish M Journal Front Cardiovasc Med Abstract Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies world (show more...)Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies worldwide. Biomarker(s) for the disorder exists, but while these have excellent negative predictive value, their positive predictive value is poor. Extracellular vesicles released by the placenta into the maternal circulation, syncytiotrophoblast membrane extracellular vesicles (STB-EVs), have been identified as being involved in PE with the potential to act as liquid biopsies. (hide) EV-METRIC 78% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type maternal placenta perfusate Sample origin Control condition Focus vesicles small extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Filtration Protein markers EV: Alix/ CD63/ PLAP/ CD9 non-EV: Cytochrome C Proteomics yes Show all info Study aim Biomarker/Identification of content (omics approaches) Sample Species Homo sapiens Sample Type maternal placenta perfusate Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed Yes Pelleting: time(min) 120 Pelleting: rotor type Sorvall TST28.39 Pelleting: speed (g) 150,000 Wash: volume per pellet (ml) 45 Wash: time (min) 120 Wash: Rotor Type Sorvall TST28.39 Wash: speed (g) 150,000 Filtration steps 0.2 or 0.22 _m Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) 2250-3040 Western Blot Detected EV-associated proteins Alix/ CD63/ PLAP Not detected EV-associated proteins CD9 Not detected contaminants Cytochrome C Flow cytometry Type of Flow cytometry A BD LSRII flow cytometer (BD Biosciences) with a blue, violet, and red laser Calibration bead size 0.11/ 0.18/ 0.24/ 0.30/ 0.50/ 0.59/ 0.88/ 1.30 Proteomics database PRIDE Characterization: RNA analysis RNA analysis Type (RT)-(q)PCR/ RNA-sequencing/ Capillary electrophoresis (e.g. Bioanalyzer) Database NCBI geo Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Modus Reported size (nm) 479 EV concentration Yes Particle yield 1.59E11-1.78E11 EM EM-type Transmission-EM Image type Close-up, Wide-field

	EV210382	3/4	Homo sapiens	maternal placenta perfusate	(d)(U)C	Awoyemi T	2023	78%
Study summary Full title A cross-sectional analysis of syncytiotrophoblast membrane extracellular vesicles-derived transcriptomic biomarkers in early-onset preeclampsia. All authors Awoyemi T, Zhang W, Rahbar M, Cribbs A, Logenthiran P, Jiang S, Collett G, Cerdeira AS, Vatish M Journal Front Cardiovasc Med Abstract Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies world (show more...)Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies worldwide. Biomarker(s) for the disorder exists, but while these have excellent negative predictive value, their positive predictive value is poor. Extracellular vesicles released by the placenta into the maternal circulation, syncytiotrophoblast membrane extracellular vesicles (STB-EVs), have been identified as being involved in PE with the potential to act as liquid biopsies. (hide) EV-METRIC 78% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type maternal placenta perfusate Sample origin Preeclampsia Focus vesicles medium/large extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: CD63/ PLAP/ CD9 non-EV: Cytochrome C Proteomics yes Show all info Study aim Biomarker/Identification of content (omics approaches) Sample Species Homo sapiens Sample Type maternal placenta perfusate Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed Yes Pelleting: time(min) 30 Pelleting: rotor type Sorvall TST28.39 Pelleting: speed (g) 10,000 Wash: volume per pellet (ml) 45 Wash: time (min) 30 Wash: Rotor Type Sorvall TST28.39 Wash: speed (g) 10,000 Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) 10460-21650 Western Blot Detected EV-associated proteins CD63/ PLAP Not detected EV-associated proteins CD9 Not detected contaminants Cytochrome C Proteomics database PRIDE Characterization: RNA analysis RNA analysis Type (RT)-(q)PCR/ RNA-sequencing/ Capillary electrophoresis (e.g. Bioanalyzer) Database NCBI geo Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Modus Reported size (nm) 205.8 EV concentration Yes Particle yield 3.75E11-1E12 EM EM-type Transmission-EM Image type Close-up, Wide-field

	EV210382	4/4	Homo sapiens	maternal placenta perfusate	(d)(U)C Filtration	Awoyemi T	2023	78%
Study summary Full title A cross-sectional analysis of syncytiotrophoblast membrane extracellular vesicles-derived transcriptomic biomarkers in early-onset preeclampsia. All authors Awoyemi T, Zhang W, Rahbar M, Cribbs A, Logenthiran P, Jiang S, Collett G, Cerdeira AS, Vatish M Journal Front Cardiovasc Med Abstract Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies world (show more...)Preeclampsia (PE) is a pregnancy-specific hypertensive disorder affecting 2%-8% of pregnancies worldwide. Biomarker(s) for the disorder exists, but while these have excellent negative predictive value, their positive predictive value is poor. Extracellular vesicles released by the placenta into the maternal circulation, syncytiotrophoblast membrane extracellular vesicles (STB-EVs), have been identified as being involved in PE with the potential to act as liquid biopsies. (hide) EV-METRIC 78% (50th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type maternal placenta perfusate Sample origin Preeclampsia Focus vesicles small extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Filtration Protein markers EV: Alix/ CD63/ PLAP/ CD9 non-EV: Cytochrome C Proteomics yes Show all info Study aim Biomarker/Identification of content (omics approaches) Sample Species Homo sapiens Sample Type maternal placenta perfusate Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed Yes Pelleting: time(min) 120 Pelleting: rotor type Sorvall TST28.39 Pelleting: speed (g) 150,000 Wash: volume per pellet (ml) 45 Wash: time (min) 120 Wash: Rotor Type Sorvall TST28.39 Wash: speed (g) 150,000 Filtration steps 0.2 or 0.22 _m Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) 5780-10648 Western Blot Detected EV-associated proteins Alix/ CD63/ PLAP Not detected EV-associated proteins CD9 Not detected contaminants Cytochrome C Flow cytometry Type of Flow cytometry A BD LSRII flow cytometer (BD Biosciences) with a blue, violet, and red laser Calibration bead size 0.11/ 0.18/ 0.24/ 0.30/ 0.50/ 0.59/ 0.88/ 1.30 Proteomics database PRIDE Characterization: RNA analysis RNA analysis Type (RT)-(q)PCR/ RNA-sequencing/ Capillary electrophoresis (e.g. Bioanalyzer) Database NCBI geo Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Modus Reported size (nm) 479 EV concentration Yes Particle yield 2.2E11-7.2E11 EM EM-type Transmission-EM Image type Close-up, Wide-field

				1 - 4 of 4

EV-TRACK ID	EV210382
species	Homo sapiens
sample type	maternal placenta perfusate
condition	Control condition	Control condition	Preeclampsia	Preeclampsia
separation protocol	dUC	dUC/ Filtration	dUC	dUC/ Filtration
vesicle related term	medium/large EV	small EV	medium/large EV	small EV
Exp. nr.	1	2	3	4
EV-METRIC %	78	78	78	78