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You searched for: EV210297 (EV-TRACK ID)
Showing 1 - 7 of 7
Showing 1 - 7 of 7
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210297 | 1/7 | Mus musculus | M1 |
qEV IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 50% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
50% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
CD9truc-EGFP expressing
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
qEV
Immunoaffinity capture (non-commercial) PEG precipitation Protein markers
EV: Alix/ Flotillin-1/ EGFP/ TSG101
non-EV: Actin/ Lamin A/C Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
M1
EV-harvesting Medium
Serum free medium
Cell viability (%)
80
Separation Method
Commercial kit
qEV
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
qEV
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ Flotillin-1/ EGFP/ TSG101
Not detected contaminants
Actin/ Lamin A/C
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Size range/distribution
Reported size (nm)
30-150
|
||||||||
EV210297 | 2/7 | Mus musculus | Blood plasma |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
CD9truc-EGFPxCMV-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: Alix/ EGFP
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
EGFP
Not detected EV-associated proteins
Alix
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210297 | 3/7 | Mus musculus | Blood plasma |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
CD9truc-EGFPxPax8-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: Alix/ EGFP
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Not detected EV-associated proteins
Alix/ EGFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210297 | 4/7 | Mus musculus | Blood plasma |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
CD9truc-EGFPx?MHC-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: Alix/ EGFP
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
Blood plasma
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ EGFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210297 | 5/7 | Mus musculus | urine |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (55th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
urine
Sample origin
CD9truc-EGFPxCMV-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: CD81
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
urine
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210297 | 6/7 | Mus musculus | urine |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (55th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
urine
Sample origin
CD9truc-EGFPxPax8-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: CD81/ EGFP
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
urine
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD81/ EGFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210297 | 7/7 | Mus musculus | urine |
IAF PEG precipitation |
Nørgård, Mikkel | 2022 | 25% | |
Study summaryFull title
All authors
Mikkel Ø. Nørgård, Lasse B. Steffensen, Didde R. Hansen, Ernst-Martin Füchtbauer, Morten B. Engelund, Henrik Dimke, Ditte C. Andersen & Per Svenningsen
Journal
Sci Rep
Abstract
The in vivo function of cell-derived extracellular vesicles (EVs) is challenging to establish since (show more...)
EV-METRIC
25% (55th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
urine
Sample origin
CD9truc-EGFPx?MHC-Cre
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Immunoaffinity capture (non-commercial)
PEG precipitation Protein markers
EV: CD81/ EGFP
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Mus musculus
Sample Type
urine
Separation Method
Immunoaffinity capture
Selected surface protein(s)
EGFP
Other
Name other separation method
PEG precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Not detected EV-associated proteins
CD81/ EGFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 7 of 7 |
EV-TRACK ID | EV210297 | ||||||
---|---|---|---|---|---|---|---|
species | Mus musculus | ||||||
sample type | Cell culture | Blood plasma | Blood plasma | Blood plasma | urine | urine | urine |
cell type | M1 | NA | NA | NA | NA | NA | NA |
medium | Serum free medium | NA | NA | NA | NA | NA | NA |
condition | CD9truc-EGFP expressing | CD9truc-EGFPxCMV-Cre | CD9truc-EGFPxPax8-Cre | CD9truc-EGFPx?MHC-Cre | CD9truc-EGFPxCMV-Cre | CD9truc-EGFPxPax8-Cre | CD9truc-EGFPx?MHC-Cre |
separation protocol | qEV/ IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation | IAF capture (non-commercial)/ PEG precipitation |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
EV-METRIC % | 50 | 25 | 25 | 25 | 25 | 25 | 25 |