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You searched for: EV210254 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210254 | 1/3 | Homo sapiens | MC65 |
(d)(U)C Filtration |
Imanbekova, Meruyert | 2021 | 50% | |
Study summaryFull title
All authors
Meruyert Imanbekova, Sorina Suarasan, Tatu Rojalin, Rachel R. Mizenko, Silvia Hilt, Meghna Mathur, Paula Lepine, Michael Nicouleau, Nguyen-Vi Mohamed, Thomas M. Durcan, Randy P. Carney, John C. Voss and Sebastian Wachsmann-Hogiu
Journal
Nanoscale advances
Abstract
One of the hallmarks of Alzheimer's disease (AD) pathogenesis is believed to be the production and d (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ CD63/ CD81/ AB
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MC65
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
120000
Filtration steps
Between 0.1 and 0.2 ?m
Characterization: Protein analysis
Protein Concentration Method
Not determined
Detected EV-associated proteins
AB
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
157.3 nm
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
150
|
||||||||
EV210254 | 2/3 | Homo sapiens | MC65 |
(d)(U)C Filtration |
Imanbekova, Meruyert | 2021 | 50% | |
Study summaryFull title
All authors
Meruyert Imanbekova, Sorina Suarasan, Tatu Rojalin, Rachel R. Mizenko, Silvia Hilt, Meghna Mathur, Paula Lepine, Michael Nicouleau, Nguyen-Vi Mohamed, Thomas M. Durcan, Randy P. Carney, John C. Voss and Sebastian Wachsmann-Hogiu
Journal
Nanoscale advances
Abstract
One of the hallmarks of Alzheimer's disease (AD) pathogenesis is believed to be the production and d (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Tetracycline-treated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ CD63/ CD81/ AB
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MC65
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
120000
Filtration steps
Between 0.1 and 0.2 ?m
Characterization: Protein analysis
Protein Concentration Method
Not determined
Detected EV-associated proteins
AB
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
164.1 nm
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
150
|
||||||||
EV210254 | 3/3 | Homo sapiens | midbrain organoids |
(d)(U)C Filtration |
Imanbekova, Meruyert | 2021 | 50% | |
Study summaryFull title
All authors
Meruyert Imanbekova, Sorina Suarasan, Tatu Rojalin, Rachel R. Mizenko, Silvia Hilt, Meghna Mathur, Paula Lepine, Michael Nicouleau, Nguyen-Vi Mohamed, Thomas M. Durcan, Randy P. Carney, John C. Voss and Sebastian Wachsmann-Hogiu
Journal
Nanoscale advances
Abstract
One of the hallmarks of Alzheimer's disease (AD) pathogenesis is believed to be the production and d (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD9/ CD63/ CD81/ AB
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
midbrain organoids
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW 28
Pelleting: speed (g)
120000
Wash: volume per pellet (ml)
Not specified
Wash: time (min)
90
Wash: Rotor Type
SW 28
Wash: speed (g)
120000
Filtration steps
Between 0.1 and 0.2 ?m
Characterization: Protein analysis
Protein Concentration Method
Not determined
Detected EV-associated proteins
AB
Detected EV-associated proteins
CD9/ CD63/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
293.5 nm
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
150
|
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1 - 3 of 3 |
EV-TRACK ID | EV210254 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | MC65 | MC65 | midbrain organoids |
condition | Control condition | Tetracycline-treated | Control condition |
separation protocol | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration |
Exp. nr. | 1 | 2 | 3 |
EV-METRIC % | 50 | 50 | 50 |