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You searched for: EV210253 (EV-TRACK ID)
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Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210253 | 1/1 | Homo sapiens | Serum | (d)(U)C | Małys MSS | 2021 | 67% | |
Study summaryFull title
All authors
Małys MSS, Aigner C, Schulz SMM, Schachner H, Rees AJJ, Kain R
Journal
Int J Mol Sci
Abstract
Robust, well-characterized methods for purifying small extracellular vesicles (sEV) from blood are n (show more...)
EV-METRIC
67% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CD9/ CD63/ CD81/ MHC1/ MHC2/ CD19/ CD3/ CD4
non-EV: Calnexin/ Albumin/ apoB Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods/ Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SX4750 in Allegra X-15R
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
32
Wash: time (min)
120
Wash: Rotor Type
SX4750 in Allegra X-15R
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
32.4 ?g
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63
Detected contaminants
Calnexin
Not detected contaminants
Albumin
ELISA
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry specific beads
Antibody details provided?
Yes
Antibody dilution provided?
No
Selected surface protein(s)
CD9/ CD63/ CD81/ MHC1/ MHC2/ CD19/ CD3/ CD4
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
150
EV concentration
Yes
EM
EM-type
Transmission EM/ Immuno EM
EM protein
CD63/ CD9/ ApoB100/48
Image type
Close-up
|
||||||||
EV210253 | 2/1 | Homo sapiens | Serum |
(d)(U)C Exo-spin |
Małys MSS | 2021 | 67% | |
Study summaryFull title
All authors
Małys MSS, Aigner C, Schulz SMM, Schachner H, Rees AJJ, Kain R
Journal
Int J Mol Sci
Abstract
Robust, well-characterized methods for purifying small extracellular vesicles (sEV) from blood are n (show more...)
EV-METRIC
67% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Protein markers
EV: CD9/ CD63/ CD81/ MHC1/ MHC2/ CD19/ CD3/ CD4
non-EV: Calnexin/ Albumin/ apoB Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods/ Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Commercial kit
Exo-spin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
40 ?g
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63
Detected contaminants
Calnexin
Not detected contaminants
Albumin
ELISA
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry aspecific beads
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81
Flow cytometry specific beads
Antibody details provided?
Yes
Antibody dilution provided?
No
Selected surface protein(s)
CD9/ CD63/ CD81/ MHC1/ MHC2/ CD19/ CD3/ CD4
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
132
EV concentration
Yes
EM
EM-type
Transmission EM/ Immuno EM
EM protein
CD63/ CD9/ ApoB100/48
Image type
Close-up
|
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1 - 2 of 2 |
EV-TRACK ID | EV210253 | |
---|---|---|
species | Homo sapiens | |
sample type | Serum | |
condition | Control condition | |
separation protocol | dUC | dUC/ Exo-spin |
Exp. nr. | 1 | 2 |
EV-METRIC % | 67 | 67 |