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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV210251	1/1	Sus scrofa	Follicular fluid	Exo-spin	Gad A	2022	75%
Study summary Full title Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality. All authors Gad A, Murin M, Bartkova A, Kinterova V, Marcollova K, Laurincik J, Prochazka R Journal J Anim Sci Biotechnol Abstract Ovarian follicular fluids (FFs) contain several kinds of regulatory factors that maintain a suitable (show more...)Ovarian follicular fluids (FFs) contain several kinds of regulatory factors that maintain a suitable microenvironment for oocyte development. Extracellular vesicles (EVs) are among the factors that play essential roles in regulating follicle and oocyte development through their cargo molecules that include microRNAs (miRNAs). This study aimed to investigate small-EV (s-EV) miRNAs in porcine FFs and their potential association with oocyte quality. (hide) EV-METRIC 75% (96th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Follicular fluid Sample origin Control condition Focus vesicles small extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture Commercial method Protein markers EV: Alix/ CD63/ TSG101 non-EV: ATP5A/ CytC Proteomics no Show all info Study aim Biomarker/Identification of content (omics approaches) Sample Species Sus scrofa Sample Type Follicular fluid Separation Method Commercial kit Exo-spin Characterization: Protein analysis Protein Concentration Method Fluorometric assay Protein Yield (µg) particles per milliliter of starting sample Western Blot Detected EV-associated proteins Alix/ CD63/ TSG101 Not detected contaminants ATP5A/ CytC Characterization: RNA analysis RNA analysis Type Capillary electrophoresis (e.g. Bioanalyzer) Proteinase treatment No RNAse treatment No Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Median Reported size (nm) 135 EV concentration Yes Particle yield particles per milliliter of starting sample: 9.00e+9 EM EM-type Transmission EM Image type Close-up, Wide-field

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EV-TRACK ID	EV210251
species	Sus scrofa
sample type	Follicular fluid
condition	Control condition
separation protocol	Exo-spin
Exp. nr.	1
EV-METRIC %	75