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You searched for: EV210237 (EV-TRACK ID)
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Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210237 | 1/2 | Homo sapiens | Blood plasma | qEV | Gelibter S | 2022 | 75% | |
Study summaryFull title
All authors
Gelibter S, Marostica G, Mandelli A, Siciliani S, Podini P, Finardi A, Furlan R
Journal
J Extracell Vesicles
Abstract
Mounting evidence suggests that storage has an impact on extracellular vesicles (EVs) properties. Wh (show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
qEV
Protein markers
EV: Alix/ Flotillin-1/ Lamp1/ ANXA1
non-EV: GM130/ ApoE/ H3 Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Commercial kit
qEV
Other
Name other separation method
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Protein Yield (µg)
particles per milliliter of starting sample
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ Flotillin-1/ Lamp1/ ANXA1
Not detected contaminants
GM130/ ApoE/ H3
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
50-350
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.00e+11
EM
EM-type
Immuno-EM
EM protein
CD63
Image type
Wide-field
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EV210237 | 2/2 | Mus musculus | BV2 | (d)(U)C | Gelibter S | 2022 | 56% | |
Study summaryFull title
All authors
Gelibter S, Marostica G, Mandelli A, Siciliani S, Podini P, Finardi A, Furlan R
Journal
J Extracell Vesicles
Abstract
Mounting evidence suggests that storage has an impact on extracellular vesicles (EVs) properties. Wh (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NA
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ Flotillin-1/ Lamp1/ ANXA1/ IB4
non-EV: GM130/ H3 Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
BV2
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Cell count
40000000
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
4h
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
12
Wash: time (min)
960
Wash: Rotor Type
SW 41 Ti
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ Flotillin-1/ Lamp1/ ANXA1
Detected contaminants
H3
Not detected contaminants
GM130
Flow cytometry
Type of Flow cytometry
CytoflexS
Calibration bead size
0.1, 0.3, 0.5, 0.7, 0.9
Antibody details provided?
Yes
Detected EV-associated proteins
IB4
Characterization: Lipid analysis
No
Characterization: Particle analysis
TRPS
Report type
Mean
Reported size (nm)
50-350
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.00e+11
Particle analysis: flow cytometry
Flow cytometer type
CytoflexS
Hardware adjustment
Calibration bead size
0.1, 0.3, 0.5, 0.7, 0.9
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.00e+7
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1 - 2 of 2 |
EV-TRACK ID | EV210237 | |
---|---|---|
species | Homo sapiens | Mus musculus |
sample type | Blood plasma | Cell culture |
cell type | NA | BV2 |
medium | NA | EV-depleted medium |
condition | Control condition | NA |
separation protocol | qEV | dUC |
Exp. nr. | 1 | 2 |
EV-METRIC % | 75 | 56 |