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You searched for: EV210179 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210179 | 2/6 | Mus musculus | Renca |
(d)(U)C DG |
Samoylenko, Anatoliy | 2021 | 88% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ Alix/ CD9/ CD81
non-EV: Argonaute2/ GM130 Proteomics
no
EV density (g/ml)
1.07-1.12
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Renca
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
2
Orientation
Top-down
Rotor type
TH-641
Speed (g)
100000
Duration (min)
900
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: duration (min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per cell 0.17
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ Alix/ CD81
Not detected contaminants
GM130/ Argonaute2
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
108
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 9.96E+06
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Wide-field
Report size (nm)
30-200
|
||||||||
EV210179 | 4/6 | Mus musculus | Renca |
(d)(U)C DG |
Samoylenko, Anatoliy | 2021 | 88% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ TSG101/ CD9/ CD81
non-EV: Argonaute2/ GM130 Proteomics
no
EV density (g/ml)
1.07-1.12
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Renca
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
12
Sample volume (mL)
2
Orientation
Top-down
Rotor type
TH-641
Speed (g)
100000
Duration (min)
900
Fraction volume (mL)
1
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: duration (min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per cell 0.185
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ CD81
Not detected contaminants
GM130/ Argonaute2
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
128
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 2.10E+07
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Wide-field
Report size (nm)
30-200
|
||||||||
EV210179 | 1/6 | Mus musculus | Renca |
(d)(U)C Exo-Spin |
Samoylenko, Anatoliy | 2021 | 67% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD81/ TSG101/ CD9/ Alix
non-EV: Argonaute2/ GM130 Proteomics
yes
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Renca
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Commercial kit
Exo-Spin
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per million cells 1.15
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ Alix/ CD81
Not detected contaminants
GM130/ Argonaute2
Proteomics database
Yes:
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
131
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 9.07E+07
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Wide-field
Report size (nm)
30-200
|
||||||||
EV210179 | 3/6 | Mus musculus | Renca |
(d)(U)C Exo-Spin |
Samoylenko, Anatoliy | 2021 | 67% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD81/ TSG101/ CD9/ Alix
non-EV: Argonaute2/ GM130 Proteomics
yes
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Renca
EV-harvesting Medium
Serum free medium
Cell viability (%)
97
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Commercial kit
Exo-Spin
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per cell 1.53
Western Blot
Detected EV-associated proteins
Alix/ CD9/ TSG101/ CD81
Not detected contaminants
GM130/ Argonaute2
Proteomics database
Yes:
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
168
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 1.73E+08
EM
EM-type
Immuno-EM/ Transmission-EM
EM protein
CD63
Image type
Wide-field
Report size (nm)
30-200
|
||||||||
EV210179 | 5/6 | Homo sapiens | 786-O |
(d)(U)C Exo-Spin |
Samoylenko, Anatoliy | 2021 | 56% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
56% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
786-O
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Commercial kit
Exo-Spin
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per cell 1.18
Western Blot
Detected EV-associated proteins
CD81
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
177.5
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 3.74E+07
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-200
|
||||||||
EV210179 | 6/6 | Homo sapiens | 786-O |
(d)(U)C Exo-Spin |
Samoylenko, Anatoliy | 2021 | 56% | |
Study summaryFull title
All authors
Anatoliy Samoylenko, Martin Kögler, Artem Zhyvolozhnyi, Olha Makieieva, Geneviève Bart, Sampson S. Andoh, Matthieu Roussey, Seppo J. Vainio, and Jussi Hiltunen
Journal
Sci Rep
Abstract
Extracellular vesicles (EVs) represent a diverse group of small membrane-encapsulated particles invo (show more...)
EV-METRIC
56% (89th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
hypoxia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Commercial method Protein markers
EV: CD81
non-EV: GM130 Proteomics
no
Show all info
Study aim
New methodological development/Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
786-O
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Cell count
Not reported
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
900
Pelleting: rotor type
TH-641
Pelleting: speed (g)
100000
Commercial kit
Exo-Spin
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
Yes, per cell 1.43
Western Blot
Detected EV-associated proteins
CD81
Not detected contaminants
GM130
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
153.5
EV concentration
Yes
Particle yield
Yes, as number of particles per million cells 7.27E+07
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-200
|
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1 - 6 of 6 |
EV-TRACK ID | EV210179 | |||||
---|---|---|---|---|---|---|
species | Mus musculus | Mus musculus | Mus musculus | Mus musculus | Homo sapiens | Homo sapiens |
sample type | Cell culture | Cell culture | Cell culture | Cell culture | Cell culture | Cell culture |
cell type | Renca | Renca | Renca | Renca | 786-O | 786-O |
condition | Control condition | hypoxia | Control condition | hypoxia | Control condition | hypoxia |
separation protocol | (d)(U)C DG | (d)(U)C DG | (d)(U)C Exo-Spin | (d)(U)C Exo-Spin | (d)(U)C Exo-Spin | (d)(U)C Exo-Spin |
Exp. nr. | 2 | 4 | 1 | 3 | 5 | 6 |
EV-METRIC % | 88 | 88 | 67 | 67 | 56 | 56 |