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You searched for: EV210096 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210096 | 2/6 | Homo sapiens | Urine |
(d)(U)C Filtration |
Royo, Felix | 2016 | 38% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: TSG101/ CD63/ Flotillin1/ CD9/ CD26/ CD10/ AQP2/ AIP
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Type 70 Ti
Pelleting: speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD26/ CD10/ AQP2/ AIP/ TSG101
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV210096 | 5/6 | Homo sapiens | Urine |
(d)(U)C Filtration Total Exosome Isolation |
Royo, Felix | 2016 | 38% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: TSG101/ CD63/ Flotillin1/ CD9/ CD26/ CD10/ AQP2/ AIP
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
Total Exosome Isolation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD26/ CD10/ AQP2/ AIP/ CD9/ CD63/ TSG101
Not detected EV-associated proteins
Flotillin1
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV210096 | 6/6 | Homo sapiens | Urine |
(d)(U)C Filtration Extraction with biotinylated Solanum tuberosum (potato) lectin (STL) and streptavidin coated Dynabeads |
Royo, Felix | 2016 | 38% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Extraction with biotinylated Solanum tuberosum (potato) lectin (STL) and streptavidin coated Dynabeads Protein markers
EV: TSG101/ CD63/ AIP/ CD26/ CD10/ AQP2/ Flotillin1/ CD9
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Other
Name other separation method
Extraction with biotinylated Solanum tuberosum (potato) lectin (STL) and streptavidin coated Dynabeads
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD26/ CD10/ AQP2/ TSG101/ CD9/ CD63
Not detected EV-associated proteins
AIP
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV210096 | 1/6 | Homo sapiens | Urine |
(d)(U)C Filtration Urine Exosome RNA Isolation Kit (Norgen Biotek Corp) |
Royo, Felix | 2016 | 25% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Prostate cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: TSG101/ CD63/ Flotillin1/ CD9/ CD26/ CD10/ AQP2/ AIP
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
Urine Exosome RNA Isolation Kit (Norgen Biotek Corp)
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD9/ CD63/ CD26/ CD10/ AQP2/ AIP/ TSG101
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
|
||||||||
EV210096 | 3/6 | Homo sapiens | Urine |
(d)(U)C Filtration ExoQuick |
Royo, Felix | 2016 | 25% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: TSG101/ CD63/ CD10/ AQP2/ AIP/ CD26/ Flotillin1/ CD9
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD26
Not detected EV-associated proteins
Flotillin1/ CD10/ AQP2/ AIP/ TSG101
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
EV210096 | 4/6 | Homo sapiens | Urine |
(d)(U)C Filtration Urine Exosome RNA Isolation Kit (Norgen Biotek Corp) |
Royo, Felix | 2016 | 25% | |
Study summaryFull title
All authors
Felix Royo, Patricia Zuñiga-Garcia, Pilar Sanchez-Mosquera, Ainara Egia, Amparo Perez, Ana Loizaga, Raquel Arceo, Isabel Lacasa, Ainara Rabade, Edurne Arrieta, Roberto Bilbao, Miguel Unda, Arkaitz Carracedo, Juan M Falcon-Perez
Journal
J Extracell Vesicles
Abstract
Urine sample analysis is irreplaceable as a non-invasive method for disease diagnosis and follow-up. (show more...)
EV-METRIC
25% (56th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Commercial method Protein markers
EV: TSG101/ CD63/ Flotillin1/ CD9/ CD26/ CD10/ AQP2/ AIP
non-EV: Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Commercial kit
Urine Exosome RNA Isolation Kit (Norgen Biotek Corp)
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin1/ CD26/ CD10/ AQP2/ AIP/ CD9/ CD63/ TSG101
Detected contaminants
Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;Capillary electrophoresis (e.g. Bioanalyzer)
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Cryo-EM
Image type
Wide-field
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV210096 | |||||
---|---|---|---|---|---|---|
species | Homo sapiens | |||||
sample type | Urine | |||||
condition | Control condition | Control condition | Control condition | Prostate cancer | Control condition | Control condition |
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration Total Exosome Isolation | (d)(U)C Filtration Extraction with biotinylated Solanum tuberosum (potato) lectin (STL) and streptavidin coated Dynabeads | (d)(U)C Filtration Urine Exosome RNA Isolation Kit (Norgen Biotek Corp) | (d)(U)C Filtration ExoQuick | (d)(U)C Filtration Urine Exosome RNA Isolation Kit (Norgen Biotek Corp) |
Exp. nr. | 2 | 5 | 6 | 1 | 3 | 4 |
EV-METRIC % | 38 | 38 | 38 | 25 | 25 | 25 |