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You searched for: EV200094 (EV-TRACK ID)
Showing 1 - 7 of 7
Showing 1 - 7 of 7
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200094 | 1/4 | Vibrio cholerae | C6706 |
(d)(U)C UF Filtration |
Qing S | 2020 | 29% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Filtration No extra separation steps Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
C6706
EV-harvesting Medium
Serum free medium
Cell count
1,30E+13
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
110000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Other
Name other separation method
No extra separation steps
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
43,6
|
||||||||
EV200094 | 2/4 | Shigella flexneri | strain 301 |
(d)(U)C UF Filtration |
Qing S | 2020 | 29% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Filtration No extra separation steps Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Shigella flexneri
Sample Type
Cell culture supernatant
EV-producing cells
strain 301
EV-harvesting Medium
Serum free medium
Cell count
1,41E+13
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
110000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Other
Name other separation method
No extra separation steps
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
43,2
|
||||||||
EV200094 | 3/4 | Escherichia coli | BL21 |
(d)(U)C UF Filtration |
Qing S | 2020 | 29% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Filtration No extra separation steps Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
BL21
EV-harvesting Medium
Serum free medium
Cell count
8,36E+12
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
110000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Other
Name other separation method
No extra separation steps
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
42,5
|
||||||||
EV200094 | 4/4 | Escherichia coli | DH5 |
(d)(U)C UF Filtration |
Qing S | 2020 | 29% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
29% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ outer membrane vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Ultrafiltration Filtration No extra separation steps Protein markers
EV: None
non-EV: None Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
DH5
EV-harvesting Medium
Serum free medium
Cell count
4,15E+12
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 28
Pelleting: speed (g)
110000
Filtration steps
0.45µm > x > 0.22µm,
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Other
Name other separation method
No extra separation steps
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
45
|
||||||||
EV200094 | 1/3 | Vibrio cholerae | / | not specified | Qing S | 2020 | 0% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other
Separation protocol
Separation protocol
not specified
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Vibrio cholerae
Sample Type
Cell culture supernatant
EV-producing cells
/
Separation Method
Other
Name other separation method
not specified
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
40
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200094 | 2/3 | Shigella flexneri | / | NA | Qing S | 2020 | 0% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Separation protocol
Separation protocol
NA
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Shigella flexneri
Sample Type
Cell culture supernatant
EV-producing cells
/
Separation Method
Other
Name other separation method
NA
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV200094 | 3/3 | Escherichia coli | DH5 | NA | Qing S | 2020 | 0% | |
Study summaryFull title
All authors
Qing S, Lyu C, Zhu L, Pan C, Wang S, Li F, Wang J, Yue H, Gao X, Jia R, Wei W, Ma G
Journal
Adv Mater
Abstract
The highly immunosuppressive tumor microenvironment (TME) in solid tumors often dampens the efficacy (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Separation protocol
Separation protocol
NA
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
DH5
Separation Method
Other
Name other separation method
NA
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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