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You searched for: EV200089 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200089 | 1/1 | Homo sapiens | red blood cells |
(d)(U)C DC Filtration |
Zhang, Gensheng; Huang, Xiaofa | 2020 | 67% | |
Study summaryFull title
All authors
Gensheng Zhang, Xiaofang Huang, Huiqing Xiu, Yan Sun, Jiming Chen, Guoping Cheng, Zhengbo Song, Yanmei Peng, Yingying Shen, Jianli Wang, Zhijian Cai
Journal
J Extracell Vesicles
Abstract
Extracellular vesicles (EVs) are excellent potential vectors for the delivery of therapeutic drugs. (show more...)
EV-METRIC
67% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
red blood cells
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Protein markers
EV: TSG101/ HSP70/ Alix
non-EV: ARF6 Proteomics
no
Show all info
Study aim
Function/Biomarker/Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
red blood cells
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
35
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
TSG101/ HSP70/ Alix
Not detected contaminants
ARF6
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Other
Reported size (nm)
154+/-51
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 1.84E+12
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
150-200
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1 - 1 of 1 |
EV-TRACK ID | EV200089 |
---|---|
species | Homo sapiens |
sample type | red blood cells |
condition | Control condition |
separation protocol | (d)(U)C DC Filtration |
Exp. nr. | 1 |
EV-METRIC % | 67 |