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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV200066	1/4	Mus musculus	Primary choroid plexus epithelial cells	qEV	Vandendriessche, Charysse	2021	75%
Study summary Full title Importance of extracellular vesicle secretion at the blood–cerebrospinal fluid interface in the pathogenesis of Alzheimer’s disease All authors Charysse Vandendriessche, Sriram Balusu, Caroline Van Cauwenberghe, Marjana Brkic, Marie Pauwels, Nele Plehiers, Arnout Bruggeman, Pieter Dujardin, Griet Van Imschoot, Elien Van Wonterghem, An Hendrix, Femke Baeke, Riet De Rycke, Kris Gevaert & Roosmarijn E. Vandenbroucke Journal Acta neuropathologica communications Abstract Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathog (show more...)Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathogenesis of Alzheimer’s disease (AD). We previously reported that the blood–cerebrospinal fluid (CSF) interface, formed by the choroid plexus epithelial (CPE) cells, releases an increased amount of EVs into the CSF in response to peripheral inflammation. Here, we studied the importance of CP-mediated EV release in AD pathogenesis. We observed increased EV levels in the CSF of young transgenic APP/PS1 mice which correlated with high amyloid beta (Aβ) CSF levels at this age. The intracerebroventricular (icv) injection of Aβ oligomers (AβO) in wild-type mice revealed a significant increase of EVs in the CSF, signifying that the presence of CSF-AβO is sufficient to induce increased EV secretion. Using in vivo, in vitro and ex vivo approaches, we identified the CP as a major source of the CSF-EVs. Interestingly, AβO-induced, CP-derived EVs induced pro-inflammatory effects in mixed cortical cultures. Proteome analysis of these EVs revealed the presence of several pro-inflammatory proteins, including the complement protein C3. Strikingly, inhibition of EV production using GW4869 resulted in protection against acute AβO-induced cognitive decline. Further research into the underlying mechanisms of this EV secretion might open up novel therapeutic strategies to impact the pathogenesis and progression of AD. (hide) EV-METRIC 75% (96th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture Commercial method No extra separation steps Protein markers EV: CD81/ CD9/ TSG101 non-EV: None Proteomics yes Show all info Study aim Function/Identification of content (omics approaches) Sample Species Mus musculus Sample Type Cell culture supernatant EV-producing cells Primary choroid plexus epithelial cells EV-harvesting Medium Serum free medium Separation Method Commercial kit qEV Other Name other separation method No extra separation steps Characterization: Protein analysis Protein Concentration Method microBCA Western Blot Detected EV-associated proteins CD81/ TSG101 Not detected contaminants Calnexin Proteomics database No Detected EV-associated proteins CD9/ CD81 Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Size range/distribution EV concentration Yes Particle yield Yes, as number of particles per milliliter of starting sample F2: 9.15E09;F3: 4.42E09 EM EM-type Transmission-EM Image type Close-up, Wide-field

	EV200066	3/4	Mus musculus	Choroid plexus explants	qEV	Vandendriessche, Charysse	2021	75%
Study summary Full title Importance of extracellular vesicle secretion at the blood–cerebrospinal fluid interface in the pathogenesis of Alzheimer’s disease All authors Charysse Vandendriessche, Sriram Balusu, Caroline Van Cauwenberghe, Marjana Brkic, Marie Pauwels, Nele Plehiers, Arnout Bruggeman, Pieter Dujardin, Griet Van Imschoot, Elien Van Wonterghem, An Hendrix, Femke Baeke, Riet De Rycke, Kris Gevaert & Roosmarijn E. Vandenbroucke Journal Acta neuropathologica communications Abstract Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathog (show more...)Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathogenesis of Alzheimer’s disease (AD). We previously reported that the blood–cerebrospinal fluid (CSF) interface, formed by the choroid plexus epithelial (CPE) cells, releases an increased amount of EVs into the CSF in response to peripheral inflammation. Here, we studied the importance of CP-mediated EV release in AD pathogenesis. We observed increased EV levels in the CSF of young transgenic APP/PS1 mice which correlated with high amyloid beta (Aβ) CSF levels at this age. The intracerebroventricular (icv) injection of Aβ oligomers (AβO) in wild-type mice revealed a significant increase of EVs in the CSF, signifying that the presence of CSF-AβO is sufficient to induce increased EV secretion. Using in vivo, in vitro and ex vivo approaches, we identified the CP as a major source of the CSF-EVs. Interestingly, AβO-induced, CP-derived EVs induced pro-inflammatory effects in mixed cortical cultures. Proteome analysis of these EVs revealed the presence of several pro-inflammatory proteins, including the complement protein C3. Strikingly, inhibition of EV production using GW4869 resulted in protection against acute AβO-induced cognitive decline. Further research into the underlying mechanisms of this EV secretion might open up novel therapeutic strategies to impact the pathogenesis and progression of AD. (hide) EV-METRIC 75% (96th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture Commercial method No extra separation steps Protein markers EV: CD81/ CD9/ TSG101 non-EV: None Proteomics no Show all info Study aim Function/Identification of content (omics approaches) Sample Species Mus musculus Sample Type Cell culture supernatant EV-producing cells Choroid plexus explants EV-harvesting Medium Serum free medium Separation Method Commercial kit qEV Other Name other separation method No extra separation steps Characterization: Protein analysis Protein Concentration Method microBCA Western Blot Detected EV-associated proteins CD81/ TSG101 Not detected contaminants Calnexin Detected EV-associated proteins CD81/ CD9 Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Size range/distribution EV concentration Yes Particle yield Yes, as number of particles per milliliter of starting sample F2: 4.4E10;F3: 2.8E10 EM EM-type Transmission-EM Image type Close-up, Wide-field

	EV200066	2/4	Mus musculus	Primary choroid plexus epithelial cells	qEV	Vandendriessche, Charysse	2021	17%
Study summary Full title Importance of extracellular vesicle secretion at the blood–cerebrospinal fluid interface in the pathogenesis of Alzheimer’s disease All authors Charysse Vandendriessche, Sriram Balusu, Caroline Van Cauwenberghe, Marjana Brkic, Marie Pauwels, Nele Plehiers, Arnout Bruggeman, Pieter Dujardin, Griet Van Imschoot, Elien Van Wonterghem, An Hendrix, Femke Baeke, Riet De Rycke, Kris Gevaert & Roosmarijn E. Vandenbroucke Journal Acta neuropathologica communications Abstract Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathog (show more...)Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathogenesis of Alzheimer’s disease (AD). We previously reported that the blood–cerebrospinal fluid (CSF) interface, formed by the choroid plexus epithelial (CPE) cells, releases an increased amount of EVs into the CSF in response to peripheral inflammation. Here, we studied the importance of CP-mediated EV release in AD pathogenesis. We observed increased EV levels in the CSF of young transgenic APP/PS1 mice which correlated with high amyloid beta (Aβ) CSF levels at this age. The intracerebroventricular (icv) injection of Aβ oligomers (AβO) in wild-type mice revealed a significant increase of EVs in the CSF, signifying that the presence of CSF-AβO is sufficient to induce increased EV secretion. Using in vivo, in vitro and ex vivo approaches, we identified the CP as a major source of the CSF-EVs. Interestingly, AβO-induced, CP-derived EVs induced pro-inflammatory effects in mixed cortical cultures. Proteome analysis of these EVs revealed the presence of several pro-inflammatory proteins, including the complement protein C3. Strikingly, inhibition of EV production using GW4869 resulted in protection against acute AβO-induced cognitive decline. Further research into the underlying mechanisms of this EV secretion might open up novel therapeutic strategies to impact the pathogenesis and progression of AD. (hide) EV-METRIC 17% (53rd percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Stimulated with amyloid beta oligomers Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture Commercial method No extra separation steps Protein markers EV: None non-EV: None Proteomics yes Show all info Study aim Function/Identification of content (omics approaches) Sample Species Mus musculus Sample Type Cell culture supernatant EV-producing cells Primary choroid plexus epithelial cells EV-harvesting Medium Serum free medium Separation Method Commercial kit qEV Other Name other separation method No extra separation steps Characterization: Protein analysis Protein Concentration Method Not determined Proteomics database No Characterization: Lipid analysis No Characterization: Particle analysis None

	EV200066	4/4	Mus musculus	Choroid plexus explants	qEV	Vandendriessche, Charysse	2021	0%
Study summary Full title Importance of extracellular vesicle secretion at the blood–cerebrospinal fluid interface in the pathogenesis of Alzheimer’s disease All authors Charysse Vandendriessche, Sriram Balusu, Caroline Van Cauwenberghe, Marjana Brkic, Marie Pauwels, Nele Plehiers, Arnout Bruggeman, Pieter Dujardin, Griet Van Imschoot, Elien Van Wonterghem, An Hendrix, Femke Baeke, Riet De Rycke, Kris Gevaert & Roosmarijn E. Vandenbroucke Journal Acta neuropathologica communications Abstract Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathog (show more...)Increasing evidence indicates that extracellular vesicles (EVs) play an important role in the pathogenesis of Alzheimer’s disease (AD). We previously reported that the blood–cerebrospinal fluid (CSF) interface, formed by the choroid plexus epithelial (CPE) cells, releases an increased amount of EVs into the CSF in response to peripheral inflammation. Here, we studied the importance of CP-mediated EV release in AD pathogenesis. We observed increased EV levels in the CSF of young transgenic APP/PS1 mice which correlated with high amyloid beta (Aβ) CSF levels at this age. The intracerebroventricular (icv) injection of Aβ oligomers (AβO) in wild-type mice revealed a significant increase of EVs in the CSF, signifying that the presence of CSF-AβO is sufficient to induce increased EV secretion. Using in vivo, in vitro and ex vivo approaches, we identified the CP as a major source of the CSF-EVs. Interestingly, AβO-induced, CP-derived EVs induced pro-inflammatory effects in mixed cortical cultures. Proteome analysis of these EVs revealed the presence of several pro-inflammatory proteins, including the complement protein C3. Strikingly, inhibition of EV production using GW4869 resulted in protection against acute AβO-induced cognitive decline. Further research into the underlying mechanisms of this EV secretion might open up novel therapeutic strategies to impact the pathogenesis and progression of AD. (hide) EV-METRIC 0% (median: 14% of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Cell culture supernatant Sample origin Stimulated with amyloid beta oligomers Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture Commercial method No extra separation steps Protein markers EV: None non-EV: None Proteomics no Show all info Study aim Function/Identification of content (omics approaches) Sample Species Mus musculus Sample Type Cell culture supernatant EV-producing cells Choroid plexus explants EV-harvesting Medium Serum free medium Separation Method Commercial kit qEV Other Name other separation method No extra separation steps Characterization: Protein analysis None Protein Concentration Method Not determined Characterization: Lipid analysis No Characterization: Particle analysis None

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EV-TRACK ID	EV200066
species	Mus musculus
sample type	Cell culture
cell type	Primary choroid plexus epithelial cells	Choroid plexus explants	Primary choroid plexus epithelial cells	Choroid plexus explants
condition	Control condition	Control condition	Stimulated with amyloid beta oligomers	Stimulated with amyloid beta oligomers
separation protocol	qEV No extra separation steps	qEV No extra separation steps	qEV No extra separation steps	qEV No extra separation steps
Exp. nr.	1	3	2	4
EV-METRIC %	75	75	17	0