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You searched for: EV200030 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200030 | 1/2 | Homo sapiens | oral mucosa lamina propria progenitor cells |
(d)(U)C Other/ ExoSpin UF Filtration DG |
Knight R | 2022 | 100% | |
Study summaryFull title
All authors
Knight R, Board-Davies E, Brown H, Clayton A, Davis T, Karatas B, Burston J, Tabi Z, Falcon-Perez JM, Paisey S, Stephens P
Journal
Stem Cells Transl Med
Abstract
Scar formation during wound repair can be devastating for affected individuals. Our group previously (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Filtration Density gradient Protein markers
EV: CD81/ CD63/ CD9
non-EV: CD105/ CD90/ CD166 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
oral mucosa lamina propria progenitor cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.2M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
5
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
MLS-50
Speed (g)
200000
Duration (min)
16
Fraction volume (mL)
0.3
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Other/ ExoSpin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Flow cytometry aspecific beads
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
CD90/ CD105/ CD166
Flow cytometry specific beads
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
94
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: 7.7E+12
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
||||||||
EV200030 | 2/2 | Homo sapiens | bone marrow derived mesenchymal stromal cells |
(d)(U)C Other/ ExoSpin UF Filtration DG |
Knight R | 2022 | 100% | |
Study summaryFull title
All authors
Knight R, Board-Davies E, Brown H, Clayton A, Davis T, Karatas B, Burston J, Tabi Z, Falcon-Perez JM, Paisey S, Stephens P
Journal
Stem Cells Transl Med
Abstract
Scar formation during wound repair can be devastating for affected individuals. Our group previously (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other/ small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Ultrafiltration Filtration Density gradient Protein markers
EV: CD81/ CD63/ CD9
non-EV: None Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
bone marrow derived mesenchymal stromal cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
0.2M
Highest density fraction
2.5M
Total gradient volume, incl. sample (mL)
5
Sample volume (mL)
0.2
Orientation
Bottom-up
Rotor type
MLS-50
Speed (g)
200000
Duration (min)
16
Fraction volume (mL)
0.3
Fraction processing
None
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Polyethersulfone (PES)
Commercial kit
Other/ ExoSpin
Characterization: Protein analysis
Protein Concentration Method
microBCA
Flow cytometry aspecific beads
Detected EV-associated proteins
CD9/ CD63/ CD81
Not detected contaminants
CD90/ CD105/ CD166
Flow cytometry specific beads
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
99.5
EV concentration
Yes
Particle yield
as number of particles per milliliter of starting sample: 4.27E+12
EM
EM-type
Cryo-EM
Image type
Close-up, Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV200030 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | oral mucosa lamina propria progenitor cells | bone marrow derived mesenchymal stromal cells |
condition | Control condition | Control condition |
separation protocol | dUC/ Other/ ExoSpin/ Ultrafiltration/ Filtration/ Density gradient | dUC/ Other/ ExoSpin/ Ultrafiltration/ Filtration/ Density gradient |
Exp. nr. | 1 | 2 |
EV-METRIC % | 100 | 100 |