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You searched for: EV190092 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190092 | 2/2 | Homo sapiens | HT1080 | (d)(U)C | Brassart B | 2019 | 44% | |
Study summaryFull title
All authors
Brassart B, Da Silva J, Donet M, Seurat E, Hague F, Terryn C, Velard F, Michel J, Ouadid-Ahidouch H, Monboisse JC, Hinek A, Maquart FX, Ramont L, Brassart-Pasco S.
Journal
Br J Cancer
Abstract
BACKGROUND:
Carcinogenesis occurs in elastin-rich tissues and leads to local inflammation and elasto (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
elastin degradation product-stimulated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD9/ CD81/ RhoA/ HSP90/ Actin/ Integrin-alphaV/ P-ERM/ MMP-2/ MMP-14
non-EV: GM130 Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT1080
EV-harvesting Medium
Serum free medium
Cell viability (%)
94
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
JLA110
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Wash: time (min)
Wash: Rotor Type
Wash: speed (g)
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD81/ RhoA/ HSP90/ Actin/ Integrin-alphaV/ P-ERM/ MMP-2/ MMP-14
Not detected contaminants
GM130
Fluorescent NTA
Relevant measurements variables specified?
NA
Antibody details provided?
No
Detected EV-associated proteins
Not detected contaminants
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
EV concentration
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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EV190092 | 1/2 | Homo sapiens | HT1080 | (d)(U)C | Brassart B | 2019 | 14% | |
Study summaryFull title
All authors
Brassart B, Da Silva J, Donet M, Seurat E, Hague F, Terryn C, Velard F, Michel J, Ouadid-Ahidouch H, Monboisse JC, Hinek A, Maquart FX, Ramont L, Brassart-Pasco S.
Journal
Br J Cancer
Abstract
BACKGROUND:
Carcinogenesis occurs in elastin-rich tissues and leads to local inflammation and elasto (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ HSP90/ CD63/ CD9
non-EV: GM130 Proteomics
no
Show all info
Study aim
Biogenesis/cargo sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT1080
EV-harvesting Medium
Serum free medium
Cell viability (%)
94
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
JLA110
Pelleting: speed (g)
100000
Wash: volume per pellet (ml)
Wash: time (min)
Wash: Rotor Type
JLA110
Wash: speed (g)
Characterization: Protein analysis
None
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Detected contaminants
Not detected contaminants
Fluorescent NTA
Relevant measurements variables specified?
NA
Antibody details provided?
No
Detected EV-associated proteins
Not detected contaminants
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
0.5
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
EM
EM-type
Report size (nm)
EV concentration
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1 - 2 of 2 |
EV-TRACK ID | EV190092 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | HT1080 | |
condition | elastin degradation product-stimulated | Control condition |
separation protocol | (d)(U)C | (d)(U)C |
Exp. nr. | 2 | 1 |
EV-METRIC % | 44 | 14 |