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You searched for: EV190007 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190007 | 1/5 | Homo sapiens | Urine |
DG (d)(U)C UF |
Mussack V | 2019 | 100% | |
Study summaryFull title
All authors
Mussack V, Wittmann G, Pfaffl MW.
Journal
Biomol Detect Quanti
Abstract
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication th (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C UF Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ Syntenin/ EPCAM/ HSP70/ CD9
non-EV: Calnexin/ Tamm-Horsfall protein Proteomics
no
EV density (g/ml)
1.18-1.24
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW 60 Ti
Pelleting: speed (g)
100000
Density gradient
Only used for validation of main results
Yes
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
9.75
Sample volume (mL)
0.75
Orientation
Top-down
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
0.9
Fraction processing
Centrifugation
Pelleting: volume per fraction
4
Pelleting: duration (min)
60
Pelleting: rotor type
SW 60 Ti
Pelleting: speed (g)
100000
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ Syntenin/ TSG101/ Alix
Not detected EV-associated proteins
HSP70/ CD81/ EPCAM/ CD63
Detected contaminants
Tamm-Horsfall protein
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
150
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190007 | 2/5 | Homo sapiens | Urine |
(d)(U)C Norgen Biotek Urine Exosome Purification Kit UF |
Mussack V | 2019 | 75% | |
Study summaryFull title
All authors
Mussack V, Wittmann G, Pfaffl MW.
Journal
Biomol Detect Quanti
Abstract
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication th (show more...)
EV-METRIC
75% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Norgen Biotek Urine Exosome Purification Kit UF Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ Syntenin/ EPCAM/ HSP70/ CD9
non-EV: Calnexin/ Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
Other;Norgen Biotek Urine Exosome Purification Kit
Other
Name other separation method
Norgen Biotek Urine Exosome Purification Kit
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Alix
Not detected EV-associated proteins
HSP70/ CD81/ Syntenin/ EPCAM/ TSG101/ CD63/ CD9
Not detected contaminants
Calnexin/ Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
130
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190007 | 3/5 | Homo sapiens | Urine |
(d)(U)C Miltenyi Biotec Exosome Isolation Kit Pan UF |
Mussack V | 2019 | 75% | |
Study summaryFull title
All authors
Mussack V, Wittmann G, Pfaffl MW.
Journal
Biomol Detect Quanti
Abstract
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication th (show more...)
EV-METRIC
75% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Miltenyi Biotec Exosome Isolation Kit Pan UF Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ Syntenin/ EPCAM/ HSP70/ CD9
non-EV: Calnexin/ Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
Other;Miltenyi Biotec Exosome Isolation Kit Pan
Other
Name other separation method
Miltenyi Biotec Exosome Isolation Kit Pan
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Alix/ CD9/ CD63/ TSG101/ Syntenin/ CD81
Not detected EV-associated proteins
HSP70/ EPCAM
Not detected contaminants
Calnexin/ Tamm-Horsfall protein
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
150
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190007 | 4/5 | Homo sapiens | Urine |
(d)(U)C Qiagen exoRNeasy Serum/Plasma Midi Kit UF |
Mussack V | 2019 | 75% | |
Study summaryFull title
All authors
Mussack V, Wittmann G, Pfaffl MW.
Journal
Biomol Detect Quanti
Abstract
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication th (show more...)
EV-METRIC
75% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Qiagen exoRNeasy Serum/Plasma Midi Kit UF Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ Syntenin/ EPCAM/ HSP70/ CD9
non-EV: Calnexin/ Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
Other;Qiagen exoRNeasy Serum/Plasma Midi Kit
Other
Name other separation method
Qiagen exoRNeasy Serum/Plasma Midi Kit
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
Alix/ Syntenin
Not detected EV-associated proteins
HSP70/ CD81/ EPCAM/ TSG101/ CD63/ CD9
Detected contaminants
Tamm-Horsfall protein
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
120
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV190007 | 5/5 | Homo sapiens | Urine |
(d)(U)C Exiqon miRCURY Exosome Isolation Kit UF |
Mussack V | 2019 | 75% | |
Study summaryFull title
All authors
Mussack V, Wittmann G, Pfaffl MW.
Journal
Biomol Detect Quanti
Abstract
Small extracellular vesicles (EVs) are 50-200 nm sized mediators in intercellular communication th (show more...)
EV-METRIC
75% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Exiqon miRCURY Exosome Isolation Kit UF Protein markers
EV: TSG101/ CD63/ CD81/ Alix/ Syntenin/ EPCAM/ HSP70/ CD9
non-EV: Calnexin/ Tamm-Horsfall protein Proteomics
no
Show all info
Study aim
Identification of content (omics approaches)/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
Other;Exiqon miRCURY Exosome Isolation Kit
Other
Name other separation method
Exiqon miRCURY Exosome Isolation Kit
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ TSG101/ Alix/ CD81
Not detected EV-associated proteins
HSP70/ EPCAM/ Syntenin/ CD63
Detected contaminants
Tamm-Horsfall protein
Not detected contaminants
Calnexin
Characterization: RNA analysis
RNA analysis
Type
RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
145
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
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1 - 5 of 5 |
EV-TRACK ID | EV190007 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Urine | ||||
condition | Control condition | ||||
separation protocol | DG (d)(U)C UF | (d)(U)C Norgen Biotek Urine Exosome Purification Kit UF | (d)(U)C Miltenyi Biotec Exosome Isolation Kit Pan UF | (d)(U)C Qiagen exoRNeasy Serum/Plasma Midi Kit UF | (d)(U)C Exiqon miRCURY Exosome Isolation Kit UF |
Exp. nr. | 1 | 2 | 3 | 4 | 5 |
EV-METRIC % | 100 | 75 | 75 | 75 | 75 |