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You searched for: EV190002 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV190002 | 1/6 | Homo sapiens | MIA PaCa-2 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 78% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: TSG101/ CD63/ MFGE8/ CD81/ Alix/ HSP70/ beta-actin
non-EV: Histon H2B Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MIA PaCa-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
< 1.06 g/ml
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD63/ MFGE8/ beta-actin/ HSP70/ CD81
Not detected EV-associated proteins
TSG101/ Alix
Not detected contaminants
Histon H2B
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
136
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
height: 39.1, diameter: 135.9
|
||||||||
EV190002 | 2/6 | Homo sapiens | MIA PaCa-2 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 78% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: TSG101/ CD63/ MFGE8/ CD81/ Alix/ HSP70/ beta-actin
non-EV: Histon H2B Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MIA PaCa-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
ca. 1.1 g/ml
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ CD63/ MFGE8/ beta-actin/ TSG101/ HSP70/ CD81
Not detected contaminants
Histon H2B
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
124
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
height: 30.1, diameter: 96.4
|
||||||||
EV190002 | 3/6 | Homo sapiens | MIA PaCa-2 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 78% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: TSG101/ Src/ CD63/ MFGE8/ CD81/ Alix/ HSP70/ beta-actin/ CD9
non-EV: Argonaute2/ Histon H2B Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MIA PaCa-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
< 1.06 g/ml
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
CD9/ CD63/ HSP70/ MFGE8/ Src/ beta-actin/ CD81
Not detected EV-associated proteins
TSG101/ Alix
Not detected contaminants
Histon H2B/ Argonaute2
Detected EV-associated proteins
CD63
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
148
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
height: 50.5, length: 190.0
|
||||||||
EV190002 | 4/6 | Homo sapiens | MIA PaCa-2 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 78% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
78% (97th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: TSG101/ Src/ CD63/ MFGE8/ CD81/ Alix/ HSP70/ beta-actin/ CD9
non-EV: Argonaute2/ Histon H2B Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MIA PaCa-2
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
ca. 1.1 g/ml
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Detected EV-associated proteins
Alix/ MFGE8/ Src/ beta-actin/ CD9/ CD63/ TSG101/ HSP70/ CD81
Not detected contaminants
Histon H2B/ Argonaute2
Detected EV-associated proteins
CD9/ CD63
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
127
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
Report size (nm)
height 32.1, length: 120.0
|
||||||||
EV190002 | 5/6 | Homo sapiens | HT-29 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 67% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: CD81/ EpCAM/ CD63/ CD9
non-EV: Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT-29
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
< 1.06 g/ml
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
EpCAM/ CD9
Not detected EV-associated proteins
CD81/ CD63
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
162
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
|
||||||||
EV190002 | 6/6 | Homo sapiens | HT-29 |
DG (d)(U)C Filtration |
Sachiko Matsumura | 2018 | 67% | |
Study summaryFull title
All authors
Sachiko Matsumura, Tamiko Minamisawa, Kanako Suga, Hiromi Kishita, Takanori Akagi, Takanori Ichiki, Yuki Ichikawa & Kiyotaka Shiba
Journal
J Extracell Vesicles
Abstract
Phosphatidylserine (PS) has skewed distributions in the plasma membrane and is preferentially locate (show more...)
EV-METRIC
67% (94th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Other / small extracellular vesicles
Separation protocol
Separation protocol
DG
(d)(U)C Filtration Protein markers
EV: CD81/ EpCAM/ CD63/ CD9
non-EV: Proteomics
no
EV density (g/ml)
1.05-1.1
Show all info
Study aim
Biomarker/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT-29
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Density gradient
Type
Continuous
Lowest density fraction
8%
Highest density fraction
40%
Total gradient volume, incl. sample (mL)
32
Sample volume (mL)
2
Orientation
Bottom-up
Rotor type
SW 32 Ti
Speed (g)
100000
Duration (min)
1020
Fraction volume (mL)
3.2
Fraction processing
Centrifugation
Pelleting: volume per fraction
33.2
Pelleting: duration (min)
120
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
160000
Filtration steps
0.22µm or 0.2µm
EV-subtype
Distinction between multiple subtypes
Density
Used subtypes
ca. 1.1 g/ml
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Detected EV-associated proteins
CD9/ CD63/ EpCAM/ CD81
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
142
EV concentration
Yes
EM
EM-type
Atomic force-EM
Image type
Wide-field
|
||||||||
1 - 6 of 6 |
EV-TRACK ID | EV190002 | |||||
---|---|---|---|---|---|---|
species | Homo sapiens | |||||
sample type | Cell culture | |||||
cell type | MIA PaCa-2 | MIA PaCa-2 | MIA PaCa-2 | MIA PaCa-2 | HT-29 | HT-29 |
condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | DG (d)(U)C Filtration | DG (d)(U)C Filtration | DG (d)(U)C Filtration | DG (d)(U)C Filtration | DG (d)(U)C Filtration | DG (d)(U)C Filtration |
EV subtype | < 1.06 g/ml | ca. 1.1 g/ml | < 1.06 g/ml | ca. 1.1 g/ml | < 1.06 g/ml | ca. 1.1 g/ml |
Exp. nr. | 1 | 2 | 3 | 4 | 5 | 6 |
EV-METRIC % | 78 | 78 | 78 | 78 | 67 | 67 |