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You searched for: EV180067 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180067 | 1/5 | Mus musculus | MPI cells |
UF (d)(U)C qEV DG |
Deville S | 2022 | 88% | |
Study summaryFull title
All authors
Deville S, Garcia Romeu H, Oeyen E, Mertens I, Nelissen I, Salvati A
Journal
Int J Mol Sci
Abstract
Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Commercial method Density gradient Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
MPI cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1
Not detected contaminants
Cytochrome C/ GRP94
Flow cytometry
Type of Flow cytometry
BD Influx instrument/ CytoFlex
Hardware adaptation to ~100nm EV's
Both BD Influx instrument (with a small particle detector) and Beckman Coulter CytoFlex instrument have been validated for the measurement of EVs.
Calibration bead size
0.1-0.9 µm beads
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
116
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection.
Calibration bead size
0.1
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV180067 | 2/5 | Mus musculus | MPI cells |
UF (d)(U)C qEV DG |
Deville S | 2022 | 88% | |
Study summaryFull title
All authors
Deville S, Garcia Romeu H, Oeyen E, Mertens I, Nelissen I, Salvati A
Journal
Int J Mol Sci
Abstract
Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
LPS
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Commercial method Density gradient Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
MPI cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1
Not detected contaminants
Cytochrome C/ GRP94
Flow cytometry
Type of Flow cytometry
BD Influx instrument/ CytoFlex
Hardware adaptation to ~100nm EV's
Both BD Influx instrument (with a small particle detector) and Beckman Coulter CytoFlex instrument have been validated for the measurement of EVs.
Calibration bead size
0.1-0.9 µm beads
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
114
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection.
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV180067 | 3/5 | Mus musculus | MPI cells |
UF (d)(U)C qEV DG |
Deville S | 2022 | 88% | |
Study summaryFull title
All authors
Deville S, Garcia Romeu H, Oeyen E, Mertens I, Nelissen I, Salvati A
Journal
Int J Mol Sci
Abstract
Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NH2-PS NPs
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Commercial method Density gradient Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
MPI cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1
Not detected contaminants
Cytochrome C/ GRP94
Flow cytometry
Type of Flow cytometry
BD Influx instrument/ CytoFlex
Hardware adaptation to ~100nm EV's
Both BD Influx instrument (with a small particle detector) and Beckman Coulter CytoFlex instrument have been validated for the measurement of EVs.
Calibration bead size
0.1-0.9 µm beads
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
139
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection.
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
EM
Image type
Close-up, Wide-field
|
||||||||
EV180067 | 4/5 | Mus musculus | MPI cells |
UF (d)(U)C qEV DG |
Deville S | 2022 | 88% | |
Study summaryFull title
All authors
Deville S, Garcia Romeu H, Oeyen E, Mertens I, Nelissen I, Salvati A
Journal
Int J Mol Sci
Abstract
Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
COOH-PS NPs
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Commercial method Density gradient Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
MPI cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1
Not detected contaminants
GRP94/ Cytochrome C
Flow cytometry
Type of Flow cytometry
BD Influx instrument/ CytoFlex
Hardware adaptation to ~100nm EV's
Both BD Influx instrument (with a small particle detector) and Beckman Coulter CytoFlex instrument have been validated for the measurement of EVs.
Calibration bead size
0.1-0.9 µm beads
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
112
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
BD Influx flow cytometer equipped with a high power 488-nm laser (200 mW) and a small-particle detector for high sensitivity forward scatter detection.
Calibration bead size
0.1
Report type
Not Reported
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV180067 | 5/5 | Mus musculus | MPI cells |
UF (d)(U)C qEV DG |
Deville S | 2022 | 88% | |
Study summaryFull title
All authors
Deville S, Garcia Romeu H, Oeyen E, Mertens I, Nelissen I, Salvati A
Journal
Int J Mol Sci
Abstract
Extracellular vesicles are membrane-bound carriers with complex cargoes, which play a major role in (show more...)
EV-METRIC
88% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
SiO2 NPs
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Ultrafiltration
(Differential) (ultra)centrifugation Commercial method Density gradient Protein markers
EV: Alix/ CD81/ Flotillin1/ CD9
non-EV: Cytochrome C/ GRP94 Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
MPI cells
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Type
Continuous
Lowest density fraction
5%
Highest density fraction
60%
Total gradient volume, incl. sample (mL)
4.8
Sample volume (mL)
0.3
Orientation
Bottom-up
Rotor type
SW 55 Ti
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
qEV
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Detected EV-associated proteins
Alix/ CD81/ Flotillin1
Not detected contaminants
GRP94/ Cytochrome c
Flow cytometry
Type of Flow cytometry
BD Influx instrument/ CytoFlex
Hardware adaptation to ~100nm EV's
Both BD Influx instrument (with a small particle detector) and Beckman Coulter CytoFlex instrument have been validated for the measurement of EVs.
Calibration bead size
0.1-0.9 µm beads
Detected EV-associated proteins
CD9
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
102
EV concentration
Yes
Particle analysis: flow cytometry
Flow cytometer type
BD Influx
Hardware adjustment
Calibration bead size
0.1
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
1 - 5 of 5 |
EV-TRACK ID | EV180067 | ||||
---|---|---|---|---|---|
species | Mus musculus | ||||
sample type | Cell culture | ||||
cell type | MPI cells | ||||
condition | Control condition | LPS | NH2-PS NPs | COOH-PS NPs | SiO2 NPs |
separation protocol | Ultrafiltration/ dUC/ qEV/ Density gradient | Ultrafiltration/ dUC/ qEV/ Density gradient | Ultrafiltration/ dUC/ qEV/ Density gradient | Ultrafiltration/ dUC/ qEV/ Density gradient | Ultrafiltration/ dUC/ qEV/ Density gradient |
Exp. nr. | 1 | 2 | 3 | 4 | 5 |
EV-METRIC % | 88 | 88 | 88 | 88 | 88 |