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You searched for: EV180059 (EV-TRACK ID)
Showing 1 - 6 of 6
Showing 1 - 6 of 6
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180059 | 6/6 | Gut microbiota | Stool |
DG SEC |
Tulkens J | 2018 | 87% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
87% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Stool
Sample origin
Control condition
Focus vesicles
gut bacteria-derived EV
Separation protocol
Separation protocol
DG
SEC Protein markers
EV: Proteomics/ Western blot/ LPS/ OmpA/ Limulus Amebocyte Lysate Assay
non-EV: Alix/ flotilin-1/ CD9 Proteomics
yes
EV density (g/ml)
1.141-1.186
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gut microbiota
Sample Type
Stool
Separation Method
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10
Highest density fraction
50
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
0.667
Orientation
Bottom-up
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Size-exclusion chromatography
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Detected EV-associated proteins
OmpA
Not detected contaminants
Alix/ flotilin-1/ CD9
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-300
EV concentration
Yes
Particle yield
particles/ml start sample
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV180059 | 1/6 | Gram-negative bacteria | Escherichia coli Nissle 1917 |
DG SEC |
Tulkens J | 2018 | 75% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Escherichia coli
Separation protocol
Separation protocol
DG
SEC Protein markers
EV: Limulus Amebocyte Lysate assay/ LPS/ OmpA/ Western blot
non-EV: Proteomics
no
EV density (g/ml)
1.141-1.186
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gram-negative bacteria
Sample Type
Cell culture supernatant
EV-producing cells
Escherichia coli Nissle 1917
EV-harvesting Medium
Serum free medium
Separation Method
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
10
Highest density fraction
50
Total gradient volume, incl. sample (mL)
16.5
Sample volume (mL)
0.667
Orientation
Bottom-up
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Centrifugation
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Detected EV-associated proteins
OmpA/ LPS
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
50-300
EV concentration
Yes
EM
EM-type
Immuno-EM
EM protein
LPS
Image type
Close-up, Wide-field
|
||||||||
EV180059 | 2/6 | Gut microbiota | Blood plasma |
DG SEC |
Tulkens J | 2018 | 42% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
42% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
LPS-positive bacterial EV
Separation protocol
Separation protocol
DG
SEC Protein markers
EV: LPS/ OmpA
non-EV: Proteomics
no
EV density (g/ml)
1.141-1.186
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gut microbiota
Sample Type
Blood plasma
Separation Method
Density gradient
Type
Discontinuous
Number of initial discontinuous layers
4
Lowest density fraction
5
Highest density fraction
40
Total gradient volume, incl. sample (mL)
16,5
Sample volume (mL)
1
Orientation
Top-down
Rotor type
SW 32.1 Ti
Speed (g)
100000
Duration (min)
1080
Fraction volume (mL)
1
Fraction processing
Size-exclusion chromatography
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
|
||||||||
EV180059 | 3/6 | Gut microbiota | Blood plasma | SEC | Tulkens J | 2018 | 42% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
42% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Inflammatory bowel disease
Focus vesicles
LPS-positive bacterial EV
Separation protocol
Separation protocol
SEC
Protein markers
EV: LPS/ OmpA
non-EV: Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gut microbiota
Sample Type
Blood plasma
Separation Method
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Immuno-EM
EM protein
LPS
Image type
Close-up
|
||||||||
EV180059 | 5/6 | Gut microbiota | Blood plasma | SEC | Tulkens J | 2018 | 42% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
42% (74th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Therapy-induced intestinal mucositis
Focus vesicles
LPS-positive bacterial EV
Separation protocol
Separation protocol
SEC
Protein markers
EV: LPS/ OmpA
non-EV: Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gut microbiota
Sample Type
Blood plasma
Separation Method
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
Characterization: Particle analysis
EM
EM-type
Immuno-EM
EM protein
E. coli LPS
Image type
Close-up
|
||||||||
EV180059 | 4/6 | Gut microbiota | Blood plasma | SEC | Tulkens J | 2018 | 28% | |
Study summaryFull title
All authors
Tulkens J, Vergauwen G, Van Deun J, Geeurickx E, Dhondt B, Lippens L, De Scheerder MA, Miinalainen I, Rappu P, De Geest BG, Vandecasteele K, Laukens D, Vandekerckhove L, Denys H, Vandesompele J, De Wever O, Hendrix A.
Journal
Gut
Abstract
(show more...)
EV-METRIC
28% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
HIV
Focus vesicles
LPS-positive bacterial EV
Separation protocol
Separation protocol
SEC
Protein markers
EV: LPS/ OmpA
non-EV: Proteomics
no
Show all info
Study aim
Function/Biomarker/Identification of content (omics approaches)
Sample
Species
Gut microbiota
Sample Type
Blood plasma
Separation Method
Size-exclusion chromatography
Total column volume (mL)
10
Sample volume/column (mL)
2
Resin type
Sepharose CL-2B
Characterization: Protein analysis
Protein Concentration Method
Fluorometric assay (e.g. Qubit, NanoOrange,...)
Western Blot
Proteomics database
No
Detected EV-associated proteins
LPS
Characterization: Lipid analysis
No
|
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1 - 6 of 6 |
EV-TRACK ID | EV180059 | |||||
---|---|---|---|---|---|---|
species | Gut microbiota | Gram-negative bacteria | Gut microbiota | Gut microbiota | Gut microbiota | Gut microbiota |
sample type | Stool | Cell culture | Blood plasma | Blood plasma | Blood plasma | Blood plasma |
cell type | NA | Escherichia coli Nissle 1917 | NA | NA | NA | NA |
medium | NA | Serum free medium | NA | NA | NA | NA |
condition | Control condition | Control condition | Control condition | Inflammatory bowel disease | Therapy-induced intestinal mucositis | HIV |
separation protocol | DG SEC | DG SEC | DG SEC | SEC | SEC | SEC |
vesicle related term | gut bacteria-derived EV | Escherichia coli | LPS-positive bacterial EV | LPS-positive bacterial EV | LPS-positive bacterial EV | LPS-positive bacterial EV |
Exp. nr. | 6 | 1 | 2 | 3 | 5 | 4 |
EV-METRIC % | 87 | 75 | 42 | 42 | 42 | 28 |