Search > Results
You searched for: EV180041 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180041 | 1/1 | Mus musculus | 4T1 |
DG (d)(U)C |
Keklikoglou I | 2018 | 100% | |
Study summaryFull title
All authors
Keklikoglou I, Cianciaruso C, Güç E, Squadrito ML, Spring LM, Tazzyman S, Lambein L, Poissonnier A, Ferraro GB, Baer C, Cassará A, Guichard A, Iruela-Arispe ML, Lewis CE, Coussens LM, Bardia A, Jain RK, Pollard JW, De Palma M
Journal
Nat Cell Biol
Abstract
Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental s (show more...)
EV-METRIC
100% (99th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DG
(d)(U)C Adj. k-factor
191 (pelleting) / 191 (washing)
Protein markers
EV: CD81/ CD9/ Syntenin-1
non-EV: Gp96 Proteomics
yes
Show all info
Study aim
Function, Biomarker, Biogenesis/cargo sorting, Mechanism of uptake/transfer, Identification of content (omics approaches)
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
4T1
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
NA
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
134000
Pelleting: adjusted k-factor
191.0
Wash: time (min)
70
Wash: Rotor Type
SW 32 Ti
Wash: speed (g)
134000
Wash: adjusted k-factor
191.0
Density gradient
Only used for validation of main results
Yes
Type
Continuous
Lowest density fraction
10
Highest density fraction
50
Sample volume (mL)
0.1
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 40 Ti
Speed (g)
100000
Duration (min)
70
Fraction volume (mL)
2
Fraction processing
Centrifugation
Pelleting: volume per fraction
10
Pelleting: duration (min)
70
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
110000
Pelleting: adjusted k-factor
251.4
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
7.3
Western Blot
Detected EV-associated proteins
CD9, CD81, Syntenin-1
Not detected contaminants
Gp96
ELISA
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Median
Reported size (nm)
140
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV180041 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | 4T1 |
condition | Control condition |
separation protocol | DG (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 100 |