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You searched for: EV180024 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV180024 | 1/2 | Homo sapiens | THP-1 |
(d)(U)C Filtration |
Rice TF | 2018 | 55% | |
Study summaryFull title
All authors
Rice TF, Donaldson B, Bouqueau M, Kampmann B, Holder B.
Journal
Placenta
Abstract
The placenta sheds extracellular vesicles (EVs), including exosomes, into the maternal circulation. (show more...)
EV-METRIC
55% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
PMA-stimulated
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
228.8 (pelleting)
Protein markers
EV: Flotillin-1/ MHC1/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP-1
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
F-28/50
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
228.8
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
3.24
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin-1, MHC1, TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
111
EV concentration
Yes
Particle yield
1400000000
Extra information
Cells were checked for viability visually, but as they are adherent and impossible to get off the plate in good numbers whilst preserving viability, it is not possible to report viability accurately at time of EV collection. Both mode and median size were reported.
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EV180024 | 2/2 | Homo sapiens | THP-1 |
(d)(U)C Filtration |
Rice TF | 2018 | 55% | |
Study summaryFull title
All authors
Rice TF, Donaldson B, Bouqueau M, Kampmann B, Holder B.
Journal
Placenta
Abstract
The placenta sheds extracellular vesicles (EVs), including exosomes, into the maternal circulation. (show more...)
EV-METRIC
55% (88th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
228.8 (pelleting)
Protein markers
EV: Flotillin-1/ MHC1/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
THP-1
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
F-28/50
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
228.8
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
8.44
Western Blot
Antibody details provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin-1, MHC1, TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Modus
Reported size (nm)
86
EV concentration
Yes
Particle yield
3200000000
Extra information
Cells were checked for viability visually, but as they are adherent and impossible to get off the plate in good numbers whilst preserving viability, it is not possible to report viability accurately at time of EV collection. Both mode and median size were reported.
|
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1 - 2 of 2 |
EV-TRACK ID | EV180024 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | THP-1 | |
condition | PMA-stimulated | Control condition |
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 1 | 2 |
EV-METRIC % | 55 | 55 |