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You searched for: EV170041 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170041 | 1/1 | Homo sapiens | 'SW480 Dukes' type B, colorectal adenocarcinoma;SW620, colon; derived from metastatic site: lymph node | DG | Yingkuan Shao | 2018 | 87% | |
Study summaryFull title
All authors
Yingkuan Shao, Ting Chen, Xi Zheng, Sheng Yang, Kailun Xu, Xuewen Chen, Fei Xu, Lantian Wang, Yanwei Shen, Tingyang Wang, Mengwen Zhang, Wangxiong Hu, Chenyang Ye, XiaoFang Yu, Jimin Shao, Shu Zheng
Journal
Carcinogenesis
Abstract
Liver metastases develop in more than half of the patients with colorectal cancer (CRC) and are asso (show more...)
EV-METRIC
87% (98th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
DG
Protein markers
EV: Alix/ HSP70/ CD63/ CD9
non-EV: Calreticulin Proteomics
no
Show all info
Study aim
Function, Biomarker, Mechanism of uptake/transfer, Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
'SW480 Dukes' type B, colorectal adenocarcinoma / SW620, colon / derived from metastatic site: lymph node
EV-harvesting Medium
EV-depleted serum
Preparation of EDS
overnight (16h) at >=100,000g
Cell viability (%)
NA
Separation Method
Density gradient
Only used for validation of main results
Yes
Density medium
69.83
Type
Discontinuous
Number of initial discontinuous layers
3
Lowest density fraction
0.25M
Highest density fraction
1.3M
Sample volume (mL)
1
Orientation
Bottom-up (sample migrates upwards)
Rotor type
SW 41 Ti
Speed (g)
100000
Duration (min)
150
Fraction volume (mL)
2
Fraction processing
Centrifugation
Pelleting: volume per fraction
5
Pelleting: duration (min)
150
Pelleting: rotor type
MLS-50
Pelleting: speed (g)
100000
Pelleting: adjusted k-factor
142.1
Pelleting-wash: volume per pellet (mL)
1
Pelleting-wash: duration (min)
150
Pelleting-wash: rotor type
69.83
Pelleting-wash: speed (g)
MLA-130
Pelleting-wash: adjusted k-factor
69.83
Characterization: Protein analysis
Protein Concentration Method
BCA
Protein Yield (µg)
2-5
Western Blot
Detected EV-associated proteins
Alix, CD9, CD63, HSP70
Not detected contaminants
Calreticulin
Characterization: RNA analysis
Database
Yes
Proteinase treatment
Yes
Moment of Proteinase treatment
After
Proteinase type
Proteinase K
Proteinase concentration
10
RNAse treatment
Yes
Moment of RNAse treatment
After
RNAse type
RNase A
RNAse concentration
10
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-160
EV concentration
Yes
Particle yield
1.0-2.0E11
EM
EM-type
Transmission-EM
Image type
Wide-field
Report size (nm)
30-160
EV concentration
Yes
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1 - 1 of 1 |
EV-TRACK ID | EV170041 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | 'SW480 Dukes' type B colorectal adenocarcinoma SW620 colon derived from metastatic site: lymph node |
condition | Control condition |
separation protocol | DG |
Exp. nr. | 1 |
EV-METRIC % | 87 |