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You searched for: EV170031 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV170031 | 1/1 | Homo sapiens | Serum |
(d)(U)C Filtration |
Ramanathan S | 2019 | 44% | |
Study summaryFull title
All authors
Ramanathan S, Douglas SR, Alexander GM, Shenoda BB, Barrett JE, Aradillas E, Sacan A, Ajit SK
Journal
J Transl Med
Abstract
BACKGROUND:
Therapeutic plasma exchange (PE) or plasmapheresis is an extracorporeal procedure employ (show more...)
EV-METRIC
44% (86th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Complex regional pain syndrome
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
142.9 (pelleting) / 142.9 (washing)
Protein markers
EV: CD81/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
110000
Pelleting: adjusted k-factor
142.9
Wash: time (min)
70
Wash: Rotor Type
Type 50.2 Ti
Wash: speed (g)
110000
Wash: adjusted k-factor
142.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
Lowry-based assay
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63
Characterization: RNA analysis
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
85.7
EV concentration
Yes
Particle yield
394000000000
EM
EM-type
Transmission-EM/ Immune-EM
EM protein
CD81
Image type
Close-up, Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV170031 |
---|---|
species | Homo sapiens |
sample type | Serum |
condition | Complex regional pain syndrome |
separation protocol | (d)(U)C Filtration |
Exp. nr. | 1 |
EV-METRIC % | 44 |