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You searched for: EV160017 (EV-TRACK ID)
Showing 1 - 8 of 8
Showing 1 - 8 of 8
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV160017 | 1/8 | Homo sapiens | Cal-27 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Cal-27 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNA sequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 2/8 | Homo sapiens | Cal-27 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxic condition (1% O2)
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Cal-27 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR;RNAsequencing
Database
Yes
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 4/8 | Homo sapiens | SCC-9 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SCC-9 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 5/8 | Homo sapiens | SCC-9 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Hypoxic condition (1% O2)
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SCC-9 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 7/8 | Homo sapiens | Serum | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 8/8 | Homo sapiens | Serum | ExoQuick | Li L | 2016 | 37% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
37% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Oral squamous cell carcinoma patients
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV: CD81/ CD63
non-EV: Tubulin Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ CD81
Not detected contaminants
Tubulin
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 3/8 | Homo sapiens | Cal-27 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 17% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
17% (54th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HIF-1/HIF-2 alpha knockdown + Hypoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
Cal-27 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Not Determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
EV160017 | 6/8 | Homo sapiens | SCC-9 Oral squamous cell carcinoma | ExoQuick | Li L | 2016 | 17% | |
Study summaryFull title
All authors
Li L, Li C, Wang S, Wang Z, Jiang J, Wang W, Li X, Chen J, Liu K, Li C, Zhu G.
Journal
Cancer Res
Abstract
Hypoxia is a common feature of solid tumors and is associated with aggressiveness and poor patient o (show more...)
EV-METRIC
17% (54th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
HIF-1/HIF-2 alpha knockdown + Hypoxia
Focus vesicles
exosome
Separation protocol
Separation protocol
ExoQuick
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function/Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SCC-9 Oral squamous cell carcinoma
EV-harvesting Medium
Not specified
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
None
Protein Concentration Method
Not Determined
Characterization: RNA analysis
RNA analysis
Type
(RT)(q)PCR
Database
No
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
EM
EM-type
Scanning-EM
Image type
Close-up, Wide-field
Report size (nm)
50-200
|
||||||||
1 - 8 of 8 |
EV-TRACK ID | EV160017 | |||||||
---|---|---|---|---|---|---|---|---|
species | Homo sapiens | |||||||
sample type | Cell culture | Cell culture | Cell culture | Cell culture | Serum | Serum | Cell culture | Cell culture |
cell type | Cal-27 Oral squamous cell carcinoma | Cal-27 Oral squamous cell carcinoma | SCC-9 Oral squamous cell carcinoma | SCC-9 Oral squamous cell carcinoma | NA | NA | Cal-27 Oral squamous cell carcinoma | SCC-9 Oral squamous cell carcinoma |
medium | Not specified | Not specified | Not specified | Not specified | NA | NA | Not specified | Not specified |
condition | Control condition | Hypoxic condition (1% O2) | Control condition | Hypoxic condition (1% O2) | Control condition | Oral squamous cell carcinoma patients | HIF-1/HIF-2 alpha knockdown Hypoxia | HIF-1/HIF-2 alpha knockdown Hypoxia |
separation protocol | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick | ExoQuick |
Exp. nr. | 1 | 2 | 4 | 5 | 7 | 8 | 3 | 6 |
EV-METRIC % | 37 | 37 | 37 | 37 | 37 | 37 | 17 | 17 |