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You searched for: EV160002 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV160002 | 2/3 | Homo sapiens | Extruded cells (NIH3T3) |
DC Sequential extrusion |
Lunavat TR | 2016 | 37% | |
Study summaryFull title
All authors
Lunavat TR, Jang SC, Nilsson L, Park HT, Repiska G, Lässer C, Nilsson JA, Gho YS, Lötvall J
Journal
Biomaterials
Abstract
To develop RNA-based therapeutics, it is crucial to create delivery vectors that transport the RNA m (show more...)
EV-METRIC
37% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Extruded cells (NIH3T3)
Sample origin
Control condition
Focus vesicles
Nanovesicles
Separation protocol
Separation protocol
DC
Sequential extrusion Protein markers
EV: PDGFR/ Flotillin-1/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function, New methodological development
Sample
Species
Homo sapiens
Sample Type
Extruded cells (NIH3T3)
Separation Method
Density cushion
Density medium
Iodixanol
Other
Name other separation method
Sequential extrusion
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
PDGFR, Flotillin-1, CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
180-200
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV160002 | 3/3 | Homo sapiens | Extruded cells (NIH3T3) |
DC Sequential extrusion |
Lunavat TR | 2016 | 37% | |
Study summaryFull title
All authors
Lunavat TR, Jang SC, Nilsson L, Park HT, Repiska G, Lässer C, Nilsson JA, Gho YS, Lötvall J
Journal
Biomaterials
Abstract
To develop RNA-based therapeutics, it is crucial to create delivery vectors that transport the RNA m (show more...)
EV-METRIC
37% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Extruded cells (NIH3T3)
Sample origin
cMyc shRNA
Focus vesicles
Nanovesicles
Separation protocol
Separation protocol
DC
Sequential extrusion Protein markers
EV: PDGFR/ Flotillin-1/ CD9
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Function, New methodological development
Sample
Species
Homo sapiens
Sample Type
Extruded cells (NIH3T3)
Separation Method
Density cushion
Density medium
Iodixanol
Other
Name other separation method
Sequential extrusion
Characterization: Protein analysis
Protein Concentration Method
Bradford
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
PDGFR, Flotillin-1, CD9
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
180-200
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV160002 | 1/3 | Homo sapiens | Extruded cells (U973) |
DC Sequential extrusion |
Lunavat TR | 2016 | 0% | |
Study summaryFull title
All authors
Lunavat TR, Jang SC, Nilsson L, Park HT, Repiska G, Lässer C, Nilsson JA, Gho YS, Lötvall J
Journal
Biomaterials
Abstract
To develop RNA-based therapeutics, it is crucial to create delivery vectors that transport the RNA m (show more...)
EV-METRIC
0% (median: 0% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Extruded cells (U973)
Sample origin
Control condition
Focus vesicles
Nanovesicles
Separation protocol
Separation protocol
DC
Sequential extrusion Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Function, New methodological development
Sample
Species
Homo sapiens
Sample Type
Extruded cells (U973)
Separation Method
Density cushion
Density medium
Iodixanol
Other
Name other separation method
Sequential extrusion
Protein Concentration Method
Bradford
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Modus
Reported size (nm)
150
|
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1 - 3 of 3 |
EV-TRACK ID | EV160002 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Extruded cells (NIH3T3) | Extruded cells (NIH3T3) | Extruded cells (U973) |
condition | Control condition | cMyc shRNA | Control condition |
separation protocol | DC Sequential extrusion | DC Sequential extrusion | DC Sequential extrusion |
Exp. nr. | 2 | 3 | 1 |
EV-METRIC % | 37 | 37 | 0 |