Search > Results
You searched for: EV140158 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140158 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Fuhrmann G | 2014 | 67% | |
Study summaryFull title
All authors
Fuhrmann G, Serio A, Mazo M, Nair R, Stevens MM
Journal
J Control Release
Abstract
Extracellular vesicles (EVs) are phospholipid-based particles endogenously produced by cells. Their (show more...)
EV-METRIC
67% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: CD63/ CD40/ Phosphatidylserine
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.11
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
8
Highest density fraction
60
Orientation
Bottom-up
Filtration steps
0.2µm > x > 0.1µm
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD40/ Phosphatidylserine
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD40/ Phosphatidylserine
Characterization: Particle analysis
DLS
NTA
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140158 | 1/2 | Homo sapiens | NAY | (d)(U)C | Fuhrmann G | 2014 | 0% | |
Study summaryFull title
All authors
Fuhrmann G, Serio A, Mazo M, Nair R, Stevens MM
Journal
J Control Release
Abstract
Extracellular vesicles (EVs) are phospholipid-based particles endogenously produced by cells. Their (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Particle analysis
NTA
|
||||||||
1 - 2 of 2 |
EV-TRACK ID | EV140158 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C DG Filtration | (d)(U)C |
Exp. nr. | 2 | 1 |
EV-METRIC % | 67 | 0 |