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You searched for: EV140119 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140119 | 2/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Liu Z | 2014 | 44% | |
Study summaryFull title
All authors
Liu Z, Zhang X, Yu Q, He JJ
Journal
Biochem Biophys Res Commun
Abstract
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-c (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
55.47 (pelleting)
Protein markers
EV: AChE/ CD63/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
55.47
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
6
Highest density fraction
24
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140119 | 3/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Liu Z | 2014 | 44% | |
Study summaryFull title
All authors
Liu Z, Zhang X, Yu Q, He JJ
Journal
Biochem Biophys Res Commun
Abstract
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-c (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Adj. k-factor
195.3 (pelleting)
Protein markers
EV: AChE/ CD63/ HSP70
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
195.3
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
6
Highest density fraction
24
Orientation
Top-down
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP70/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AChE
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV140119 | 1/3 | Homo sapiens | Blood plasma | DG | Liu Z | 2014 | 17% | |
Study summaryFull title
All authors
Liu Z, Zhang X, Yu Q, He JJ
Journal
Biochem Biophys Res Commun
Abstract
Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-c (show more...)
EV-METRIC
17% (45th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG
Protein markers
EV: AChE
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
6
Highest density fraction
24
Orientation
Top-down
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
AChE
Characterization: Particle analysis
None
|
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1 - 3 of 3 |
EV-TRACK ID | EV140119 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | Cell culture | Blood plasma |
cell type | NAY | NAY | NA |
medium | EV Depleted | EV Depleted | |
condition | NAY | NAY | NAY |
separation protocol | (d)(U)C DG Filtration | (d)(U)C DG Filtration | DG |
Exp. nr. | 2 | 3 | 1 |
EV-METRIC % | 44 | 44 | 17 |