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You searched for: EV140111 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140111 | 2/2 | Homo sapiens | NAY | (d)(U)C | Cypryk W | 2014 | 57% | |
Study summaryFull title
All authors
Cypryk W, Ohman T, Eskelinen EL, Matikainen S, Nyman TA
Journal
J Proteome Res
Abstract
Fungal infections (mycoses) are common diseases of varying severity that cause problems, especially (show more...)
EV-METRIC
57% (92nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
138.6 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
138.6
Wash: volume per pellet (ml)
5
Characterization: Protein analysis
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ immune EM
EM protein
Annexin1 ; 14-3-3beta
Image type
Close-up, Wide-field
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EV140111 | 1/2 | Homo sapiens | NAY | (d)(U)C | Cypryk W | 2014 | 44% | |
Study summaryFull title
All authors
Cypryk W, Ohman T, Eskelinen EL, Matikainen S, Nyman TA
Journal
J Proteome Res
Abstract
Fungal infections (mycoses) are common diseases of varying severity that cause problems, especially (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
295 (pelleting)
Protein markers
EV: Annexin1/ Tubulin/ TSG101/ Alix
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
295.0
Characterization: Protein analysis
Western Blot
Detected EV-associated proteins
Alix/ TSG101/ Annexin1/ Tubulin
ELISA
Detected EV-associated proteins
Annexin1/ Tubulin
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ immune EM
EM protein
Annexin1 ; 14-3-3beta
Image type
Close-up, Wide-field
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1 - 2 of 2 |
EV-TRACK ID | EV140111 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C | (d)(U)C |
Exp. nr. | 2 | 1 |
EV-METRIC % | 57 | 44 |