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You searched for: EV140108 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140108 | 1/1 | Acholeplasma laidlawii | Mycoplasma |
(d)(U)C DG Filtration UF |
Chernov VM | 2014 | 44% | |
Study summaryFull title
All authors
Chernov VM, Mouzykantov AA, Baranova NB, Medvedeva ES, Grygorieva TY, Trushin MV, Vishnyakov IE, Sabantsev AV, Borchsenius SN, Chernova OA
Journal
J Proteomics
Abstract
Mycoplasmas (class Mollicutes), the smallest prokaryotes capable of self-replication, as well as Arc (show more...)
EV-METRIC
44% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Mycoplasma
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Adj. k-factor
95.8 (pelleting)
Protein markers
EV: HSP20
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Acholeplasma laidlawii
Sample Type
Mycoplasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
MLA80
Pelleting: adjusted k-factor
95.8
Density gradient
Lowest density fraction
10
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP20
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSP20
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM/ scanning EM/ atomic force EM
EM protein
HSP20
Image type
Close-up, Wide-field
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EV-TRACK ID | EV140108 |
---|---|
species | Acholeplasma laidlawii |
sample type | Mycoplasma |
condition | NAY |
separation protocol | (d)(U)C DG Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 44 |