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You searched for: EV140106 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140106 | 1/2 | Prochlorococcus | Bacteria |
(d)(U)C DG Filtration UF |
Biller SJ | 2014 | 57% | |
Study summaryFull title
All authors
Biller SJ, Schubotz F, Roggensack SE, Thompson AW, Summons RE, Chisholm SW
Journal
Science
Abstract
Many heterotrophic bacteria are known to release extracellular vesicles, facilitating interactions b (show more...)
EV-METRIC
57% (95th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Bacteria
Sample origin
NAY
Focus vesicles
Bacterial vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Adj. k-factor
256 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Prochlorococcus
Sample Type
Bacteria
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
256.0
Density gradient
Only used for validation of main results
Yes
Highest density fraction
40
Orientation
Bottom-up
Rotor type
SW60
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ cryo EM/ scanning EM
Image type
Wide-field
|
||||||||
EV140106 | 2/2 | Prochlorococcus | Coastal water |
(d)(U)C DG Filtration UF |
Biller SJ | 2014 | 57% | |
Study summaryFull title
All authors
Biller SJ, Schubotz F, Roggensack SE, Thompson AW, Summons RE, Chisholm SW
Journal
Science
Abstract
Many heterotrophic bacteria are known to release extracellular vesicles, facilitating interactions b (show more...)
EV-METRIC
57% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Coastal water
Sample origin
NAY
Focus vesicles
Bacterial vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Adj. k-factor
256 (pelleting)
Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Prochlorococcus
Sample Type
Coastal water
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
256.0
Density gradient
Only used for validation of main results
Yes
Highest density fraction
40
Orientation
Bottom-up
Rotor type
SW60
Speed (g)
100000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ cryo EM/ scanning EM
Image type
Wide-field
|
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1 - 2 of 2 |
EV-TRACK ID | EV140106 | |
---|---|---|
species | Prochlorococcus | |
sample type | Bacteria | Coastal water |
condition | NAY | NAY |
separation protocol | (d)(U)C DG Filtration UF | (d)(U)C DG Filtration UF |
Exp. nr. | 1 | 2 |
EV-METRIC % | 57 | 57 |