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You searched for: EV140068 (EV-TRACK ID)
Showing 1 - 3 of 3
Showing 1 - 3 of 3
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140068 | 1/3 | Homo sapiens | NAY |
(d)(U)C Filtration |
Cvjetkovic A | 2014 | 44% | |
Study summaryFull title
All authors
Cvjetkovic A, Lötvall J, Lässer C
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Extracellular vesicles (EV), the collective term for vesicles released from cells, consi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
132.9 (pelleting)
Protein markers
EV: Calnexin/ CD81/ TSG101/ CD63/ CD9
non-EV: Cell organelle protein Proteomics
no
TEM measurements
53(median)
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
132.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ CD9/ TSG101/ Calnexin
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Calnexin
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
|
||||||||
EV140068 | 3/3 | Homo sapiens | NAY |
(d)(U)C Filtration |
Cvjetkovic A | 2014 | 44% | |
Study summaryFull title
All authors
Cvjetkovic A, Lötvall J, Lässer C
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Extracellular vesicles (EV), the collective term for vesicles released from cells, consi (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
216.9 (pelleting)
Protein markers
EV: Calnexin/ CD81/ TSG101/ CD63/ CD9
non-EV: Cell organelle protein Proteomics
no
TEM measurements
53(median)
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
114
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
216.9
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ CD9/ TSG101/ Calnexin
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Calnexin
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
|
||||||||
EV140068 | 2/3 | Homo sapiens | NAY |
(d)(U)C Filtration |
Cvjetkovic A | 2014 | 14% | |
Study summaryFull title
All authors
Cvjetkovic A, Lötvall J, Lässer C
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Extracellular vesicles (EV), the collective term for vesicles released from cells, consi (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
216.9 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
216.9
Filtration steps
0.22µm or 0.2µm
|
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1 - 3 of 3 |
EV-TRACK ID | EV140068 | ||
---|---|---|---|
species | Homo sapiens | ||
sample type | Cell culture | ||
cell type | NAY | ||
condition | NAY | ||
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 1 | 3 | 2 |
EV-METRIC % | 44 | 44 | 14 |