Search > Results
You searched for: EV140051 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140051 | 1/1 | Cryptococcus neoformans | NAY |
(d)(U)C Filtration UF |
Wolf JM | 2014 | 43% | |
Study summaryFull title
All authors
Wolf JM, Espadas-Moreno J, Luque-Garcia JL, Casadevall A
Journal
Eukaryot Cell
Abstract
Cryptococcus neoformans produces extracellular vesicles containing a variety of cargo, including vir (show more...)
EV-METRIC
43% (81st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Protein markers
EV: Urease/ Phosphatase/ Laccase
non-EV: Proteomics
yes
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Cryptococcus neoformans
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Urease/ Phosphatase/ Laccase
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Urease/ Phosphatase/ Laccase
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM/ cryo EM/ scanning EM
Image type
Close-up, Wide-field
Report size (nm)
Not reported
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV140051 |
---|---|
species | Cryptococcus neoformans |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C Filtration UF |
Exp. nr. | 1 |
EV-METRIC % | 43 |