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You searched for: EV140047 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV140047 | 2/2 | Homo sapiens | NAY |
(d)(U)C Filtration |
Gonzalez E | 2014 | 44% | |
Study summaryFull title
All authors
Gonzalez E, Piva M, Rodriguez-Suarez E, Gil D, Royo F, Elortza F, Falcon-Perez JM, Vivanco Md
Journal
PLoS One
Abstract
Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important pro (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: CD63/ CD133/ MFGE8/ CD81/ Flotilin1/ Annexin2/ CD13
non-EV: Cell organelle protein Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotilin1/ MFGE8/ CD13/ CD133/ Annexin2
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MFGE8/ CD13/ CD133/ Annexin2
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Close-up
|
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EV140047 | 1/2 | Homo sapiens | NAY |
(d)(U)C Filtration |
Gonzalez E | 2014 | 33% | |
Study summaryFull title
All authors
Gonzalez E, Piva M, Rodriguez-Suarez E, Gil D, Royo F, Elortza F, Falcon-Perez JM, Vivanco Md
Journal
PLoS One
Abstract
Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important pro (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Flotilin1/ CD63/ CD133/ CD13
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotilin1/ CD133/ CD13
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD133/ CD13
Characterization: Particle analysis
NTA
EM
EM-type
cryo EM
Image type
Wide-field
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1 - 2 of 2 |
EV-TRACK ID | EV140047 | |
---|---|---|
species | Homo sapiens | |
sample type | Cell culture | |
cell type | NAY | |
condition | NAY | |
separation protocol | (d)(U)C Filtration | (d)(U)C Filtration |
Exp. nr. | 2 | 1 |
EV-METRIC % | 44 | 33 |