Search > Results
You searched for: EV130172 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130172 | 1/1 | Homo sapiens | NAY |
(d)(U)C IAF |
Canitano A | 2013 | 22% | |
Study summaryFull title
All authors
Canitano A, Venturi G, Borghi M, Ammendolia MG, Fais S
Journal
Cancer Lett
Abstract
EBV is a human herpesvirus associated with a number of malignancies. Both lymphoblastoid cell lines (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
IAF Adj. k-factor
7991 (pelleting)
Protein markers
EV: LMP1/ CD9/ Rab5b
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
F50L24x1.5
Pelleting: adjusted k-factor
7991
Immunoaffinity capture
Selected surface protein(s)
CD9, MHC2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ Rab5b/ LMP1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Rab5b/ LMP1
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV130172 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C IAF |
Exp. nr. | 1 |
EV-METRIC % | 22 |