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You searched for: EV130143 (EV-TRACK ID)
Showing 1 - 2 of 2
Showing 1 - 2 of 2
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130143 | 1/2 | Homo sapiens | Semen | (d)(U)C | Franz C | 2013 | 14% | |
Study summaryFull title
All authors
Franz C, Böing AN, Hau CM, Montag M, Strowitzki T, Nieuwland R, Toth B
Journal
J Reprod Immunol
Abstract
Recent studies indicate that various types of vesicles, like microparticles (MP) and exosomes, are p (show more...)
EV-METRIC
14% (28th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: time(min)
30
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
TF
Image type
Close-up, Wide-field
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EV130143 | 2/2 | Homo sapiens | Semen | (d)(U)C | Franz C | 2013 | 14% | |
Study summaryFull title
All authors
Franz C, Böing AN, Hau CM, Montag M, Strowitzki T, Nieuwland R, Toth B
Journal
J Reprod Immunol
Abstract
Recent studies indicate that various types of vesicles, like microparticles (MP) and exosomes, are p (show more...)
EV-METRIC
14% (28th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
58.04 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
58.04
Characterization: Particle analysis
None
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1 - 2 of 2 |
EV-TRACK ID | EV130143 | |
---|---|---|
species | Homo sapiens | |
sample type | Semen | |
condition | NAY | |
separation protocol | (d)(U)C | (d)(U)C |
Exp. nr. | 1 | 2 |
EV-METRIC % | 14 | 14 |